Identification of novel F2-isoprostane metabolites by specific UDP-glucuronosyltransferases

Redox Biology, Год журнала: 2024, Номер 70, С. 103020 - 103020

Опубликована: Янв. 4, 2024

UDP-glucuronosyltransferases (UGTs) catalyze the conjugation of glucuronic acid with endogenous and exogenous lipophilic small molecules to facilitate their inactivation excretion from body. This represents approximately 35 % all phase II metabolic transformations. Fatty acids oxidized eicosanoid derivatives can be metabolized by UGTs. F

Язык: Английский

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Resolvin D2 and its receptor GPR18 in cardiovascular and metabolic diseases: A promising biomarker and therapeutic target

Pharmacological Research, Год журнала: 2023, Номер 195, С. 106832 - 106832

Опубликована: Июнь 24, 2023

Accumulating evidence suggests that inflammation plays an important role in the pathophysiology of initiation and progression cardiovascular metabolic diseases (CVMDs). Anti-inflammation strategies those promote resolution have gradually become potential therapeutic approaches for CVMDs. Resolvin D2 (RvD2), a specialized pro-resolving mediator, exerts anti-inflammatory pro-resolution effects through its receptor GPR18, G protein–coupled receptor. Recently, RvD2/GPR18 axis has received more attention due to protective CVMDs, including atherosclerosis, hypertension, ischaemiareperfusion, diabetes. Here, we introduce basic information about RvD2 summarize their roles different immune cells, review In summary, GPR18 play occurrence development CVMDs are biomarkers targets.

Язык: Английский

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Polyunsaturated Fatty Acid Imbalance‐A Contributor to SARS CoV‐2 Disease Severity

Journal of Nutrition and Metabolism, Год журнала: 2025, Номер 2025(1)

Опубликована: Янв. 1, 2025

Overview: SARS CoV-2 infection is accompanied by the development of acute inflammation, resolution which determines course and its outcome. If not resolved (brought back to preinjury status), inflamed state progresses a severe clinical presentation characterized uncontrolled cytokine release, systemic in some death. In disease, required balance between protective inflammation appears missing, suggesting that ω-3-derived specialized proresolving mediators (SPMs) needed for are either present or at ineffective levels compared competing ω-6 polyunsaturated fatty acid (PUFA) metabolic derivatives. Aim: To determine whether PUFA linoleic (LA) metabolites increased those infected with disease uninfected controls. Findings: Increased LA metabolites, e.g., arachidonic (AA), epoxyeicosatrienoic (EET) derivatives AA (8,9-, 11,12-, 14,15-EETs), AA-derived hydroxyeicosatetraenoic (HETE) acid, dihydroxylated diols (leukotoxin isoleukotoxin), prostaglandin E2 decreased resolving SPMs. Therapeutic treatment patients ω-3 significantly 18-HEPE (SPM precursor) EPA-derived (11,12- 14,15-diHETE), while toxic 9,10- 12,13-diHOMEs iosleukotoxin, respectively) decreased. Conclusion: Unbalanced dietary intake ω-6/ω-3 PUFAs contributed severity decreasing ω-3-dependent SPM increasing membrane-associated ferroptotic peroxidation.

Язык: Английский

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Lipids and the hallmarks of ageing: From pathology to interventions

Mechanisms of Ageing and Development, Год журнала: 2023, Номер 215, С. 111858 - 111858

Опубликована: Авг. 29, 2023

Язык: Английский

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Possibility of averting cytokine storm in SARS-COV 2 patients using specialized pro-resolving lipid mediators

Biochemical Pharmacology, Год журнала: 2023, Номер 209, С. 115437 - 115437

Опубликована: Янв. 30, 2023

Язык: Английский

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Icosapent ethyl in cardiovascular prevention: Resolution of inflammation through the eicosapentaenoic acid - resolvin E1 - ChemR23 axis

Pharmacology & Therapeutics, Год журнала: 2023, Номер 247, С. 108439 - 108439

Опубликована: Май 17, 2023

Cardiovascular outcome trials on omega-3 fatty acids have generated contradictory results but indicate a dose-dependent beneficial effect of eicosapentaenoic acid (EPA). Beneficial cardiovascular effects EPA may in addition to triglyceride lowering be mediated through alternative mechanisms action. In this review, the link between and resolution atherosclerotic inflammation is addressed. substrate for enzymatic metabolism into lipid mediator resolvin E1 (RvE1), which activates receptor ChemR23 transduce an active inflammation. This has been shown dampen immune response provide atheroprotective responses different models. The intermediate metabolite 18-HEPE emerges as biomarker towards proresolving mediators observational studies. Genetic variations within EPA-RvE1-ChemR23 axis affecting open up precision medicine identify responders non-responders fish oil supplementation. conclusion, activation contribute prevention.

Язык: Английский

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A single extraction 96-well method for LC-MS/MS quantification of urinary eicosanoids, steroids and drugs

Prostaglandins & Other Lipid Mediators, Год журнала: 2023, Номер 170, С. 106789 - 106789

Опубликована: Окт. 23, 2023

Urinary eicosanoid concentrations reflect inflammatory processes in multiple diseases and have been used as biomarkers of disease well for stratification precision medicine. However, implementation urinary profiling large-scale analyses is restricted due to sample preparation limits. Here we demonstrate a single solid-phase extraction 300 µL urine 96-well-format prostaglandins, thromboxanes, isoprostanes, cysteinyl-leukotriene E4 the linoleic acid-derived dihydroxy-octadecenoic acids (9,10- 12,13-DiHOME). A simultaneous screening protocol was also developed cortisol/cortisone 7 exogenous steroids 3 cyclooxygenase-inhibitors. Satisfactory performance quantification eicosanoids with proper internal standard demonstrated intra-plate (CV=8.5-15.1%) inter-plate (n=35) from studies (CV=22.1-34.9%). Storage stability evaluated at -20 °C, polar tetranors evidenced 50% decrease after 5 months, while remaining no significant degradation. All were stable over 3.5-years stored -80°C. This method will facilitate large scale screening.

Язык: Английский

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Dietary enrichment with fish oil improved n–3 LC-PUFA profile in aquacultured Sardinella brasiliensis fillet

Journal of Food Composition and Analysis, Год журнала: 2024, Номер 127, С. 105978 - 105978

Опубликована: Янв. 7, 2024

Язык: Английский

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The severity of COVID-19 upon hospital admission is associated with plasma omega-3 fatty acids

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Май 3, 2024

Abstract Fatty acids are precursors of inflammatory oxylipins. In the context COVID-19, an excessive production pro-inflammatory cytokines is associated with disease severity. The objective was to investigate whether baseline omega 3/omega 6 fatty ratio and oxylipins were inflammation oxidative stress in unvaccinated patients classified according severity during hospitalization. This Prospective population-based cohort study included 180 hospitalized COVID-19. into five groups their disease. Group 1 least severe 5 most severe. Three specific types acids—eicosapentaenoic acid (EPA), docosahexaenoic (DHA), arachidonic (AA)—as well as enzymatic non-enzymatic determined using chromatography coupled mass spectrometry. There no difference omega-3 omega-6 between ( p = 0.276). However, EPA/AA lower 4 compared 0.015). finding increase both C-Reactive Protein < 0.001) Interleukin-6 0.002). Furthermore, concentration F 2 -Isoprostanes higher than 0.009), while significant changes observed for other among groups. Multivariate analysis did not present any standard biomarkers, suggesting high complexity factors involved Our hypothesis confirmed terms ratio. A upon hospital admission found be Interleukin-6, leading a better prognosis SARS-CoV-2 patients. Importantly, this beneficial outcome achieved without form supplementation. trial also provides important information that can further applied reduce infections uncontrolled synthesis cytokines. Trial registration : https://clinicaltrials.gov/study/NCT04449718 —01/06/2020. ClinicalTrials.gov Identifier: NCT04449718.

Язык: Английский

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Adjunctive therapy with lipid-lowering agents in COVID-19: a systematic review and meta-analysis of randomized controlled trials

Lipids in Health and Disease, Год журнала: 2023, Номер 22(1)

Опубликована: Май 8, 2023

Abstract Background Many commonly used drugs were evaluated as repurposed treatment options since the emergence of COVID-19 pandemic. The benefit lipid-lowering agents has been controversial in this regard. In systematic review, we assessed effect these medications adjunctive therapy by inclusion randomized controlled trials (RCTs). Methods We searched four international databases including PubMed, Web Science, Scopus, and Embase for RCTs April 2023. primary outcome was mortality, while other efficacy indices considered secondary outcomes. order to estimate pooled size outcomes, considering odds ratio (OR) or standardized mean difference (SMD) 95% confidence interval (CI), random-effect meta-analyses conducted. Results Ten studies involving 2,167 patients using statins, omega-3 fatty acids, fenofibrate, PCSK9 inhibitors, nicotinamide intervention compared control placebo, included. No significant found terms mortality (OR 0.96, CI 0.58 1.59, p -value = 0.86, I 2 20.4%) length hospital stay (SMD -0.10, -0.78 0.59, 0.78, 92.4%) adding a statin standard care. trend similar fenofibrate nicotinamide. inhibition, however, led decreased an overall better prognosis. Omega-3 supplementation showed contradicting results two trials, suggesting need further evaluation. Conclusion Although some observational improved outcomes agents, our study no treatment. On hand, inhibitors can be good candidate assessment. Finally, there are major limitations use supplements treating more warranted evaluate efficacy.

Язык: Английский

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