Research Square (Research Square),
Год журнала:
2023,
Номер
unknown
Опубликована: Июль 26, 2023
Abstract
The
scarcity
of
native
periosteum
poses
a
significant
clinical
barrier
in
the
repair
critical-sized
bone
defects.
challenge
enhancing
regenerative
potential
healing
is
further
compounded
by
oxidative
stress
at
fracture
site.
However,
introduction
artificial
has
demonstrated
its
ability
to
promote
regeneration
through
provision
appropriate
mechanical
support
and
controlled
release
pro-osteogenic
factors.
In
this
study,
polylactic
acid
(PLLA)/hyaluronic
(HA)-based
nanofibrous
membrane
was
fabricated
using
coaxial
electrospinning
technique.
incorporation
irisin
into
core-shell
structure
PLLA/HA
nanofibers
(PLLA/HA@Irisin)
achieved
sustained
release.
vitro
experiments
that
PLLA/HA@Irisin
membranes
exhibited
favorable
biocompatibility.
osteogenic
differentiation
marrow
mesenchymal
stem
cells
(BMMSCs)
improved
PLLA/HA@Irisin,
as
evidenced
increase
alkaline
phosphatase
(ALP)
activity
matrix
mineralization.
Mechanistically,
significantly
enhanced
mitochondrial
function
BMMSCs
via
activation
sirtuin
3
antioxidant
pathway.
To
assess
therapeutic
effectiveness,
were
implanted
situ
calvarial
defects
rats.
results
four
eight
weeks
post-surgery
indicated
implantation
superior
efficacy
promoting
vascularized
formation,
enhancement
synthesis
development
new
blood
vessels.
our
study
indicate
electrospun
possess
characteristics
biomimetic
periosteum,
showing
for
effectively
treating
improving
maintaining
redox
homeostasis
BMMSCs.
Antioxidants,
Год журнала:
2024,
Номер
13(4), С. 413 - 413
Опубликована: Март 28, 2024
Recently
discovered
irisin,
a
member
of
the
myokines
family,
is
potential
mediator
exercise-induced
energy
metabolism
and
factor
promoting
browning
white
adipose
tissue.
Recent
evidence
indicates
that
this
myokine,
released
from
contracting
muscles,
can
mediate
beneficial
effects
exercise
on
health.
Irisin
may
be
therapeutic
agent
against
obesity
has
been
shown
to
play
an
important
role
in
protection
various
cells,
tissues,
organs
due
its
anti-inflammatory,
antioxidative,
anti-cancer
properties.
Our
aim
was
review
recent
experimental
clinical
studies
irisin
expression,
release
into
bloodstream,
tissue
targets,
contribution
protective
gastrointestinal
tract.
Particular
emphasis
placed
inflammatory
bowel
disease,
intestinal
ischemia/reperfusion
injury,
periodontitis,
other
digestive
tract
disorders,
including
carcinogenesis.
Overall,
holds
significant
as
novel
target
molecule,
offering
safe
approach
treating
diseases.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(1), С. 643 - 643
Опубликована: Янв. 4, 2024
The
improvement
of
human
living
conditions
has
led
to
an
increase
in
average
life
expectancy,
creating
a
new
social
and
medical
problem—aging,
which
diminishes
the
overall
quality
life.
aging
process
body
begins
with
activation
effector
signaling
pathways
cells,
resulting
loss
their
normal
functions
deleterious
effects
on
microenvironment.
This,
turn,
leads
chronic
inflammation
similar
transformations
neighboring
cells.
cumulative
retention
these
senescent
cells
over
prolonged
period
results
deterioration
tissues
organs,
ultimately
leading
reduced
elevated
risk
mortality.
Among
most
promising
methods
for
addressing
age-related
illnesses
are
pharmacological,
genetic,
cellular
therapies.
Elevating
activity
aging-suppressing
genes,
employing
specific
groups
native
genetically
modified
utilizing
senolytic
medications
may
offer
potential
delay
ailments
long
term.
This
review
explores
strategies
advancements
field
anti-aging
therapies
currently
under
investigation,
particular
emphasis
gene
therapy
involving
adeno-associated
vectors
cell-based
therapeutic
approaches.
Cell Proliferation,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 11, 2025
ABSTRACT
The
keystone
pathogen
Porphyromonas
gingivalis
(P.g.)
is
responsible
for
cementum
resorption
in
periodontitis;
however,
the
mechanism
involved
it
remains
unclear.
Sirtuin
3
(Sirt3)
a
NAD
+
‐dependent
protein
deacetylase
contributing
to
mitochondrial
homeostasis
and
various
cell
functions.
In
this
study,
expression
of
Sirt3
cementoblasts
was
found
be
increased
during
cementoblast
mineralisation
development,
while
decreased
gradually
under
P.g.
infection
multiplicity
infection‐dependent
manner.
Compared
with
wild
type
mice,
knockout
mice
showed
less
cellular
lower
capacity
Runx2
OCN
cementoblasts.
inhibition
by
3‐TYP
or
silencing
lentivirus
both
confirmed
impaired
cementogenesis.
Conversely,
honokiol
(HKL)
simulated
bind
applied
activate
HKL‐mediated
activation
facilitated
rescued
P.g.‐suppressed
markedly.
Superoxide
dismutase
2
(SOD2),
downstream
molecule
Sirt3,
similar
pattern
different
conditions.
Silencing
SOD2
demonstrated
restrain
mineralisation.
pan
acetylation
detected
decrease
Sirt3‐upregulating
conditions
increase
Sirt3‐downregulating
binding
also
verified.
Furthermore,
specific
SOD2‐K68
were
upregulated
downregulated
HKL
stimulation.
Moreover,
K68Q
mutation
simulating
mineralisation,
K68R
deacetylation
it.
Altogether,
deacetylates
via
K68
orchestrate
P.g.‐perturbed
cementogenesis,
Sirt3‐targeted
treatment
candidate.
Regenerative Biomaterials,
Год журнала:
2023,
Номер
11
Опубликована: Окт. 31, 2023
Abstract
The
scarcity
of
native
periosteum
poses
a
significant
clinical
barrier
in
the
repair
critical-sized
bone
defects.
challenge
enhancing
regenerative
potential
healing
is
further
compounded
by
oxidative
stress
at
fracture
site.
However,
introduction
artificial
has
demonstrated
its
ability
to
promote
regeneration
through
provision
appropriate
mechanical
support
and
controlled
release
pro-osteogenic
factors.
In
this
study,
poly
(l-lactic
acid)
(PLLA)/hyaluronic
acid
(HA)-based
nanofibrous
membrane
was
fabricated
using
coaxial
electrospinning
technique.
incorporation
irisin
into
core-shell
structure
PLLA/HA
nanofibers
(PLLA/HA@Irisin)
achieved
sustained
release.
vitro
experiments
that
PLLA/HA@Irisin
membranes
exhibited
favorable
biocompatibility.
osteogenic
differentiation
marrow
mesenchymal
stem
cells
(BMMSCs)
improved
PLLA/HA@Irisin,
as
evidenced
increase
alkaline
phosphatase
activity
matrix
mineralization.
Mechanistically,
significantly
enhanced
mitochondrial
function
BMMSCs
via
activation
sirtuin
3
antioxidant
pathway.
To
assess
therapeutic
effectiveness,
were
implanted
situ
calvarial
defects
rats.
results
4
8
weeks
post-surgery
indicated
implantation
superior
efficacy
promoting
vascularized
formation,
enhancement
synthesis
development
new
blood
vessels.
our
study
indicate
electrospun
possess
characteristics
biomimetic
periosteum,
showing
for
effectively
treating
improving
maintaining
redox
homeostasis
BMMSCs.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(14), С. 7955 - 7955
Опубликована: Июль 21, 2024
A
growing
number
of
studies
indicate
that
mitochondrial
dysfunction
serves
as
a
pathological
mechanism
for
periodontitis.
Therefore,
this
two-sample
Mendelian
randomization
(MR)
study
was
carried
out
to
explore
the
causal
associations
between
biological
function
and
periodontitis,
because
specific
nature
relationship
remains
inconclusive
in
existing
MR
studies.
Inverse
variance
weighting,
randomization-Egger,
weighted
mode,
simple
median
analyses
were
performed
assess
relationships
exposure
factors
The
results
present
revealed
association
periodontitis
medium-chain
acyl-CoA
dehydrogenase
(MCAD),
malonyl-CoA
decarboxylase
(MLYCD),
glutaredoxin
2
(Grx2),
oligoribonuclease
(ORN),
pyruvate
carboxylase
(PC).
Notably,
MCAD
MLYCD
are
causally
linked
serve
protective
factors.
However,
Grx2,
ORN,
PC
risk
Our
established
such
insights
may
provide
promising
approach
treating
via
regulation.
Premature
accumulation
of
senescent
cells
results
in
tissue
destruction,
and
it
is
one
the
potential
primary
mechanisms
underlying
accelerated
progression
diabetes
periodontitis.
However,
whether
this
characterized
phenomenon
could
account
for
periodontal
pathogenesis
under
hyperglycemic
conditions
remains
unclear.
In
study,
we
assessed
phenotypic
changes
experimental
periodontitis
conditions.
Next,
investigated
mitochondrial
function
mitophagy
pathways
cellular
senescence
vitro
vivo.
Our
findings
showed
that
significant
occurred
gingival
tissues
diabetic
mice
with
increased
expression
senescence-related
protein
p21
Research Square (Research Square),
Год журнала:
2024,
Номер
unknown
Опубликована: Апрель 3, 2024
Abstract
Whether
miR-29a-5p
is
associated
with
diabetic
retinopathy
(DR)
still
a
subject
of
ongoing
discussion.
The
current
research
examines
the
involvement
in
regulating
apoptosis,
oxidative
stress,
and
inflammation
retinal
ganglion
cells
(RGCs)
generated
by
high
glucose
(HG).
Additionally,
we
are
interested
analyzing
contribution
developing
DR.
We
obtained
peripheral
blood
samples
from
7
people
DR
14
individuals
without
Subsequently,
conducted
biochemical
indices
analyses
qRT-PCR.
randomly
divided
RGCs
into
low
groups,
HG
+
inhibitor
negative
control
groups.
SIRT3
siRNA
was
transfected
through
lipofectamine
3000
reagent.
Cell
vitality
detected
MTT;
qRT-PCR
applied
to
identify
expression;
Detection
ROS,
SOD,
MDA
levels
quantified
using
DCFH-DA,
WST-1,
colorimetric
methods,
respectively;
IL-6
TNF-α
contents
were
analyzed
utilizing
ELISA;
Dual-luciferase
gene
reporter
experiment
used
examine
if
specific
target
miR-29a-5p;
Flow
cytometry
evaluated
apoptosis
RGCs;
technique
Western
blotting
identified
expression
Caspase-3,
Bax
Bcl-2
proteins.
markedly
elevated
DR,
it
had
positive
correlations
total
cholesterol
(TC)
fasting
(FBG).
combination
miR-29a-5p,
cholesterol,
triglycerides
(TG)
exhibits
significant
diagnostic
value
for
demonstrating
both
sensitivity
specificity.
High
stimulated
worsened
cellular
inflammatory
response,
apoptotic
levels.
Transfection
protected
against
HG-induced
injury,
inflammation,
apoptosis.
Furthermore,
dual-luciferase
provided
confirmation
that
as
negatively
regulated
expression.
Notably,
knockdown
abolished
protection
inhibition
on
RGCs.
Suppression
safeguards
injury
caused
via
boosting
signaling,
which
might
provide
new
prevention
treatment
strategy
