Electrospun radially oriented berberine-PHBV nanofiber dressing patches for accelerating diabetic wound healing

Regenerative Biomaterials, Год журнала: 2024, Номер 11

Опубликована: Янв. 1, 2024

Abstract A dressing patch made of radially oriented poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanofibers was successfully manufactured with a modified electrospinning strategy. The as-electrospun PHBV nanofiber exhibited uniform and bead-free nanofibrous morphology innovative arrangement, which demonstrated to possess obviously improved mechanical property, increased surface hydrophilicity enhanced biological properties compared the control traditionally randomly pattern. Interestingly, it found that pattern could induce cell migration from periphery center along in rapid manner. To further improve biofunction patch, berberine (Beri, an isoquinoline alkaloid) two different concentrations were encapsulated into during electrospinning, present sustained drug release behavior for nearly one month. Importantly, addition Beri impart excellent anti-inflammatory property by significantly inhibiting secretion pro-inflammatory factors M1 macrophages, also showed additive influence on promoting proliferation human dermal fibroblasts (HDFs), as well growth E. coli, S. aureus C. albicans, Beri-free patch. In animal studies, electrospun loading high content accelerate healing process diabetic mouse full-thickness skin wound shortened time (100% closure rate after 18 days’ treatment) quality (improved collagen deposition, re-epithelialization neovascularization hair follicles). all, this study reported therapeutic strategy integrating physical cues multiple effective treatment hard-to-heal wounds.

Язык: Английский

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Irisin alleviates hepatic steatosis by activating the autophagic SIRT3 pathway

Chinese Medical Journal, Год журнала: 2025, Номер unknown

Опубликована: Фев. 18, 2025

Abstract Background: Disruption of hepatic lipid homeostasis leads to excessive triglyceride accumulation and the development metabolic dysfunction-associated steatotic liver disease (MASLD). Autophagy, a critical process in metabolism, is impaired MASLD pathogenesis. Irisin, skeletal muscle-driven myokine, regulates but its impact on metabolism not well understood. Here, we aimed explore role irisin steatosis underlying mechanisms involved. Methods: A high-fat diet (HFD)-induced mouse model was used, recombinant protein, herein referred as “Irisin”, intraperitoneally administered for 4 weeks evaluate effects accumulation. Liver tissues were stained with Oil red O (ORO), (TG) total cholesterol (TC) contents measured serum homogenates. The expression autophagosome marker microtubule-associated protein 1 light chain 3 (LC3), autophagy receptor sequestosome-1 (SQSTM1/p62), initiation complex unc-51-like kinase (ULK1) lysosomal functional cathepsin B via Western blotting, transcription factor EB (TFEB) analyzed immunofluorescence autophagic changes. effect flux further evaluated palmitic acid-induced HepG2 cells by measuring degradation chloroquine (CQ), analyzing colocalization LC3 lysosome-associated (LAMP1). possible mechanism examined sirtuin (SIRT3) pathway validated using overexpression SIRT3 plasmid transfection or siRNA-mediated knockdown. Student’s t -test utilized statistical analysis. Results: Irisin significantly reduces mice fed HFD, accompanied enhanced hepatocyte upregulation pathway. In cells, attenuated accumulation, which partially dependent levels. Mechanistically, treatment upregulated phosphorylated AMP-activated (AMPK), inhibited mammalian target rapamycin (mTOR) activity, promoted TFEB nucleus translocation, increased expression, degradation, alleviated steatosis. No significant changes phosphorylation ULK1 hepatocytes observed. However, when siRNA used knock down , those reversed, exacerbated. Conclusions: Our findings highlight potential therapeutic modulating potentially providing novel management MASLD. Further research needed elucidate clinical applications this approach

Язык: Английский

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Enhancing Osteogenesis through Bio‐Inspired Recombinant Coral Protein Galaxin by Targeting Mitochondrial Metabolism and ATP Production

Advanced Science, Год журнала: 2025, Номер unknown

Опубликована: Март 8, 2025

Bio-inspired coral-derived scaffolds have been adopted for bone regeneration. However, these confront challenges such as the limited osteoinductive properties, environmental concerns, and ambiguous calcification mechanisms in coral proteins. In this study, role of recombinant protein galaxin promoting osteogenesis is investigated. The observations reveal that regulates mitochondrial metabolism mouse pre-osteoblasts by interacting with β subunit ATP synthase. This ultimately promotes pre-osteoblasts. Furthermore, integrated gelatin methacryloyl (GelMA) assess osteogenic potential resulting galaxin-GelMA composites mandibular defects. emphasize distinctive regulating functionality osteogenesis. Additionally, provide prospective insights further applications bio-inspired proteins regenerative medicine.

Язык: Английский

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Structurally and Functionally Adaptive Biomimetic Periosteum: Materials, Fabrication, and Construction Strategies

Exploration, Год журнала: 2025, Номер unknown

Опубликована: Фев. 27, 2025

ABSTRACT The periosteum is crucial in the processes of bone formation, regeneration, and remodelling. Specifically, periosteal progenitor cells contribute a major force to initiation healing. Biomimetic (BP), employed for treating defects, exhibits superior outcomes terms integrity, proper vascularization, minimal heterotopic ossification when compared conventional direct graft void fillers. Therefore, BP has emerged as contemporary effective approach addressing defects. As an vivo graft, necessitates excellent biocompatibility appropriate mechanical properties. Furthermore, it should closely mirror architecture functionality natural periosteum. This review provides detailed summary recent research progress on BP, incorporating inspiring studies that future development this field. Initially, examines structure function context defect repair. Subsequently, analyzes current design concept construction comprehensive overview materials techniques constructing BP. Finally, summarizes strategies used defects from various perspectives including structural functional biomimicry, discusses latest advances research.

Язык: Английский

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Fabrication and characterization of calcium lactate gluconate electrospun novel fibrous sheet for bone tissue engineering

Emergent Materials, Год журнала: 2024, Номер unknown

Опубликована: Июль 3, 2024

Язык: Английский

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Mitochondria-targeting materials and therapies for regenerative engineering

Biomaterials, Год журнала: 2024, Номер 316, С. 123023 - 123023

Опубликована: Дек. 15, 2024

Язык: Английский

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