bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Июнь 29, 2023
Background
Endocrine
fibroblast
growth
factors
(e
FGFs
)
play
important
roles
in
various
cellular
signaling
processes
such
as
development
and
differentiation.
These
genes
were
also
found
to
be
significantly
related
several
cancer.
However,
little
is
known
about
the
role
of
e
colon
neoplasia
adenocarcinoma
(COAD).
Methods
We
performed
systematically
comprehensively
investigated
gene
expression,
DNA
methylation,
prognostic
significance,
genetic
alteration,
co-expressed
genes,
protein-protein
interaction,
small
molecules
pathway,
drug
interactions
eFGFs
based
on
TIMER2.0,
GEPIA2,
UALCAN,
OncoDB,
cBioPortal,
LinkedOmics,
STRING,
SMPDB,
htfTarget,
mirTarBase,
circBank
DGIdb
databases.
Ultimately,
correlations
expressions
between
polyp
COAD
tissues
compared
normal
mucosa
validated
using
qRT-PCR
a
cross-sectional
part
our
study.
Results
The
results
indicated
that
are
highly
expressed
COAD,
abnormal
may
promoter
methylation.
In
this
matter,
methylation
analysis
revealed
promotor
hypermethylation
FGF19
FGF21
.
Conversely,
FGF23
was
shown
have
tendency
for
hypomethylation.
Moreover,
downregulation
detrimental
survival
patients.
KEGG
pathway
analyses
eFGF
family
members
mainly
regulation
actin
cytoskeleton
and,
more
notably,
Ras
signaling,
PI3k-Akt
Rap1
cancer
pathways.
Based
results,
overexpressed
polyps
mucosa.
Additionally,
RNA
expression
markedly
elevated
adenomatous
opposed
hyperplastic
polyps.
Conclusion
Collectively,
these
findings
reveal
critical
tumorigenesis
suggest
promising
diagnostic
markers
CRC
well
discriminating
high-risk
from
low-risk
Journal of Translational Medicine,
Год журнала:
2024,
Номер
22(1)
Опубликована: Май 8, 2024
Abstract
Despite
advances
in
treatment
strategies,
colorectal
cancer
(CRC)
continues
to
cause
significant
morbidity
and
mortality,
with
mounting
evidence
a
close
link
between
immune
system
dysfunctions
issued.
Interleukin-2
receptor
gamma
(IL-2RG)
plays
pivotal
role
as
common
subunit
the
IL-2
family
cytokines
activates
JAK-STAT
pathway.
This
study
delves
into
of
within
tumor
microenvironment
investigates
potential
microRNAs
(miRNAs)
that
directly
inhibit
IL-2RG,
aiming
discern
their
impact
on
CRC
clinical
outcomes.
Bioinformatics
analysis
revealed
upregulation
IL-2RG
mRNA
TCGA-COAD
samples
showed
strong
correlations
infiltration
various
lymphocytes.
Single-cell
corroborated
these
findings,
highlighting
expression
critical
cell
subsets.
To
explore
miRNA
involvement
dysregulation,
was
isolated
from
tissues
lymphocytes
258
patients
30
healthy
controls,
cloned
pcDNA3.1/CT-GFP-TOPO
vector.
Human
embryonic
kidney
lines
(HEK-293T)
were
transfected
this
construct.
Our
research
involved
comprehensive
miRPathDB,
miRWalk,
Targetscan
databases
identify
miRNAs
associated
3′
UTR
human
IL-2RG.
The
microRNA
(miRNA)
molecules,
hsa-miR-7-5p
hsa-miR-26b-5p,
have
been
identified
potent
suppressors
patients.
Specifically,
downregulation
hsa-miR-26b-5p
has
shown
result
Prognostic
evaluation
hsa-miR-7-5p,
using
data
patient
samples,
established
higher
lower
both
poorer
Additionally,
several
long
non-coding
RNAs
(LncRNAs),
such
ZFAS1,
SOX21-AS1,
SNHG11,
SNHG16,
SNHG1,
DLX6-AS1,
GAS5,
SNHG6,
MALAT1,
which
may
act
competing
endogenous
RNA
molecules
for
IL2RG
by
sequestering
shared
hsa-miR-26b-5p.
In
summary,
investigation
underscores
utility
serum
tissue
biomarkers
predicting
prognosis
while
also
offering
promise
targets
immunotherapy
management.
Graphical
Frontiers in Oncology,
Год журнала:
2025,
Номер
14
Опубликована: Янв. 9, 2025
Osteosarcoma
(OS),
a
prevalent
metastatic
cancer
among
young
individuals,
is
associated
with
grim
prognosis.
Long
non-coding
RNAs
(lncRNAs),
including
C1QTNF1-AS1,
are
pivotal
regulators
of
cell
proliferation
and
motility.
As
an
oncogene,
C1QTNF1-AS1
implicated
in
various
tumor
types,
such
as
colorectal,
pancreatic,
hepatocellular
carcinomas,
OS.
The
aim
this
study
was
to
investigate
the
functions
underlying
mechanisms
progression
osteosarcoma.
This
investigation
focused
on
elucidating
functional
roles
OS
cells.
Bioinformatics
tools
were
utilized
identify
interaction
between
microRNA
miR-34a-5p
well
targeting
LDHA
PDK3
by
miR-34a-5p.
Dual-luciferase
reporter
assays
RNA
immunoprecipitation
employed
validate
these
interactions.
Expression
profiles
miR-34a-5p,
LDHA,
osteosarcoma
cells
analyzed
using
RT-PCR
western
blot
analyses,
revealing
their
intricate
relationships.
impact
molecules
proliferation,
invasion,
migration
assessed
through
CCK-8,
Transwell,
Cell
scratch
assay.
Moreover,
effects
aerobic
glycolysis
examined
quantifying
ATP
levels,
lactate
production,
glucose
uptake
capacity,
extracellular
acidification
rate.
findings
indicated
significant
decrease
expression
levels
compared
normal
osteoblasts.
A
parallel
downregulation
trend
also
observed
Silencing
led
marked
upregulation
cells,
which
partially
attenuated
mimics.
Functional
evaluations
demonstrated
that
suppression
accelerated
growth,
motility,
invasiveness,
Warburg
effect.
Conversely,
overexpression
mitigated
stimulatory
effects,
suggesting
regulatory
role
modulating
progression.
Our
research
emphasizes
critical
pathogenesis
(OS).
We
discovered
indirectly
upregulates
suppressing
regulator
cascade
events
promotes
enhancing
glycolytic
metabolism
supplying
energy
for
migration,
invasion.
These
suggest
potential
therapeutic
target
highlight
importance
understanding
network
involving
lncRNAs
Computer Methods in Biomechanics & Biomedical Engineering,
Год журнала:
2025,
Номер
unknown, С. 1 - 12
Опубликована: Май 13, 2025
Competitive
endogenous
RNA
(ceRNA)
network
modulation
plays
a
crucial
role
in
pathogenesis
of
colon
adenocarcinoma
(COAD).
This
study
analyzed
The
Cancer
Genome
Atlas(TCGA)
data
to
identify
151
copy
number
variation
(CNV)-driven
lncRNAs
COAD,
constructing
ceRNA
(6
lncRNAs-14
miRNAs-68
mRNAs).
Functional
enrichment
revealed
their
roles
muscle
system
processes
,
blood
vessel
development
and
extracellular
matrix
organization.
Survival
analysis
linked
LINC00941
amplification
poor
prognosis.
Two
CNV-driven
lncRNA-targeting
drugs
were
identified,
offering
insights
into
COAD
mechanisms
potential
biomarkers.
ImmunoTargets and Therapy,
Год журнала:
2024,
Номер
Volume 13, С. 643 - 659
Опубликована: Ноя. 1, 2024
Sorafenib,
an
orally
active
potent
tyrosine
kinase
inhibitor
(TKI),
represented
a
primary
treatment
in
patients
with
advanced
hepatocellular
carcinoma
(HCC).
Unfortunately,
sorafenib
resistance
was
regarded
as
huge
obstacle
for
HCC
treatment.
RNA-sequencing
including
circRNA
Sequencing
(circRNA-Seq)
circular
RNAs
(circRNAs),
miRNA
(miRNA-Seq)
microRNAs
(miRNAs),
well
mRNA
(mRNA-Seq)
mRNAs
sorafenib-resistant
cells
vs
sorafenib-sensitive
cells,
were
performed.
Then,
interaction
correlation
analysis
between
differentially
expressed
circRNAs
and
miRNAs
their
target
genes
Huh7/SOR
SMMC7721/SOR
exhibited.
The
"circRNA-miRNA-mRNA"
network
constructed
through
the
Cytoscape
software
application,
Circular
RNA
Interactome
Targetscan
prediction,
binding
protein
immunoprecipitation
(RIP),
pull-down,
Dual
luciferase
reporter
assay.
Furthermore,
mRNA-Seq,
Gene
Ontology
(GO)
function
Kyoto
Encyclopedia
of
Genes
Genomes
(KEGG)
pathway
enrichment
downstream
involved
implemented.
Iron
detection
assay,
Lipid
peroxidation
quantification
ROS
measurement
CCK-8
tumor
challenge
vivo
used
to
determine
mechanisms
promoting
HCC,
where
is
clearly
in.
circ_0001944
circ_0078607
upregulation
2
downregulated
(circ_0002874
circ_0069981),
11
upregulated
miR-193a-5p,
miR-197-3p,
miR-27a-5p,
miR-551b-5p,
miR-335-3p,
miR-767-3p,
miR-767-5p,
miR-92a-1-5p,
miR-92a-3p,
miR-3940-3p,
miR-664b-3p
3
(miR-1292-5p,
let-7c-5p,
miR-99a-5p)
determined.
Among
these
non-coding
(ncRNAs),
miR-1292-5p
should
not
be
drop
out
sight;
has
been
proved
inhibit
its
expression
HCC.
Subsequent
findings
also
raise
that
directly
targeted
3'-noncoding
region
(3'-UTR)
Fibulin
(FBLN2)
mRNA.
targets
miR-1292-5p/FBLN2
axis
cell
ferroptosis
which
indicated
regulators
associated
iron
overload
lipid
"rearranged".
Most
importantly,
mitigating
ferroptosis,
cannot
left
unrecognized.
Circ_0001944
putative
reversing
Our
are
expected
provide
new
directions
sensitization
Cell Death and Disease,
Год журнала:
2024,
Номер
15(12)
Опубликована: Дек. 18, 2024
Abstract
Colorectal
cancer
(CRC)
is
the
third
most
common
diagnosed
and
second
leading
cause
of
cancer-related
deaths.
Emerging
evidence
has
indicated
that
long
non-coding
RNAs
(lncRNAs)
are
involved
in
progression
various
types
cancer.
In
this
study,
we
aimed
to
identify
potential
causal
lncRNAs
CRC
through
comprehensive
multilevel
bioinformatics
analyses,
coupled
with
functional
validation.
Our
analyses
identified
LINC02257
as
being
highly
expressed
CRC,
associated
poor
survival
advanced
tumor
stages
among
patients
CRC.
Genome-wide
association
analysis
revealed
significant
associations
between
variants
near
suggesting
a
role
for
Network
playing
key
epithelial-mesenchymal
transition
pathway.
Single-cell
RNA
sequencing
showed
elevated
expression
was
reduced
proportion
epithelial
cells.
vitro
experiments
positively
regulated
metastatic
proliferative
Mechanistically,
affected
malignancy
by
functioning
competitive
endogenous
microRNAs
RNA-binding
proteins.
upregulated
SERPINE1
sequestering
suppressive
miR-1273g-3p,
thereby
increasing
abilities
Additionally,
directly
interacted
YB1
induced
its
phosphorylation,
facilitating
nuclear
translocation.
The
transcriptional
activation
target
genes
oncogenic
functions
.
Taken
together,
our
results
demonstrate
promising
therapeutic
treatment.