Metabolic signature for a dysbiotic microbiome in the female genital tract: A systematic review and meta‐analysis DOI Creative Commons

Biban Gill,

Ingrid Schwecht,

Nuzhat Rahman

и другие.

American Journal of Reproductive Immunology, Год журнала: 2023, Номер 90(4)

Опубликована: Сен. 27, 2023

Abstract Background The vaginal microbiome (VMB) is a critical determinant of reproductive health, where microbial shift towards dysbiotic environment has implications for susceptibility to, and clinical presentation sexually transmitted infections (STIs). Metabolomic profiling the microenvironment led to identification metabolic responses conditions dysbiosis. However, no studies have examined markers that are common across can serve as signature Method Study We conducted comprehensive systematic review meta‐analysis identify consistently deregulated metabolites along with their impact on host metabolism during employed two complementary approaches including vote counting analysis all eligible identified in review, addition subset sufficient available data. Significantly were then selected pathway enrichment analysis. Results Our results revealed total 502 altered reported 10 from 16 studies. Following rigorous, collective analysis, six which downregulated could be generalized identified. In addition, five one upregulated metabolite was bacterial vaginosis (BV) focused sub‐analysis. These potential Their role eight pathways indicates disruption amino acid, carbohydrate, energy Conclusion Based this we propose schematic model outlining perturbations associated dysbiosis, targets therapeutics prophylaxis.

Язык: Английский

Short-chain fatty acids: linking diet, the microbiome and immunity DOI
Elizabeth R. Mann, Ying Ka Lam, Holm H. Uhlig

и другие.

Nature reviews. Immunology, Год журнала: 2024, Номер 24(8), С. 577 - 595

Опубликована: Апрель 2, 2024

Язык: Английский

Процитировано

223
Cervicovaginal microbiota: a promising direction for prevention and treatment in cervical cancer DOI Creative Commons

Jie Shen,

Hao Sun, Jing Chu

и другие.

Infectious Agents and Cancer, Год журнала: 2024, Номер 19(1)

Опубликована: Апрель 19, 2024

Cervical cancer is a common malignancy in women, with high incidence rate and mortality. Persistent infection of high-risk human papillomavirus (HPV) the most important risk factor for cervical precancerous lesions. Cervicovaginal microbiota (CVM) plays an essential role defense HPV infections prevention subsequent Dominance Lactobacillus key CVM homeostasis, which can be regulated by host, exogenous endogenous factors. Dysbiosis CVM, including altered microbial, metabolic, immune signatures, contribute to persist infection, leading cancer. However, there no evidence causality between cancer, underlying mechanism remains unexplored. Considering close correlation dysbiosis persistent this review will overview its development related mechanisms, prospects therapeutic applications.

Язык: Английский

Процитировано

8
Role of CRISPR-Cas systems and anti-CRISPR proteins in bacterial antibiotic resistance DOI Creative Commons
Hiva Kadkhoda, Pourya Gholizadeh, Hossein Samadi Kafil

и другие.

Heliyon, Год журнала: 2024, Номер 10(14), С. e34692 - e34692

Опубликована: Июль 1, 2024

The emergence and development of antibiotic resistance in bacteria is a serious threat to global public health. Antibiotic genes (ARGs) are often located on mobile genetic elements (MGEs). They can be transferred among by horizontal gene transfer (HGT), leading the spread drug-resistant strains treatment failure. CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated genes) one many strategies have developed under long-term selection pressure restrict HGT. CRISPR-Cas systems exist about half bacterial genomes play significant role limiting resistance. On other hand, bacteriophages MGEs encode wide range anti-CRISPR proteins (Acrs) counteract immunity system. Acrs could decrease system's activity against phages facilitate acquisition ARGs virulence traits for bacteria. This review aimed assess relationship between with We also highlighted technology control prevent antibacterial system target nucleic acid sequences high accuracy reliability; therefore, it has become novel editing therapy tool CRISPR-based approaches may pave way developing smart antibiotics, which eliminate multidrug-resistant (MDR) distinguish pathogenic beneficial microorganisms. Additionally, engineered gene-containing combination antibiotics used as cutting-edge approach reduce

Язык: Английский

Процитировано

6
Sequencing and culture-based characterization of the vaginal and uterine microbiota in beef cattle that became pregnant or remained open following artificial insemination DOI Creative Commons

Emily M. Webb,

Devin B. Holman,

Kaycie N. Schmidt

и другие.

Microbiology Spectrum, Год журнала: 2023, Номер 11(6)

Опубликована: Ноя. 3, 2023

Emerging evidence suggests that microbiome-targeted approaches may provide a novel opportunity to reduce the incidence of reproductive failures in cattle. To develop such microbiome-based strategies, one first logical steps is identify microbiome features related fertility and isolate fertility-associated microbial species for developing future bacterial consortium could be administered before breeding enhance pregnancy outcomes. Here, we characterized vaginal uterine microbiota beef cattle became pregnant or remained open via artificial insemination identified associated with fertility. We compared similarities between heifers cows. Using culturing, provided new insights into culturable fraction their antimicrobial resistance. Overall, our findings will serve as an important basis research aimed at harnessing improved

Язык: Английский

Процитировано

14
Vaginal Sensors DOI
Kourosh Kalantar‐zadeh,

Daniella Susic,

Jon Hyett

и другие.

ACS Sensors, Год журнала: 2024, Номер 9(8), С. 3810 - 3827

Опубликована: Июль 18, 2024

The development and market emergence of vaginal sensors have begun to demonstrate their impact on women's healthcare. Until recently, in limited cases, these exhibited capabilities diagnosing monitoring disorders the tract during different stages lives. This Perspective is a compilation what has been accomplished so far landscape sensors. text explores diverse types sensor technologies, applications, potential review introduces anatomy vagina cervix categorizes that developed, highlighting technologies applications. paper covers biomarkers discusses importance maintaining overall characteristics system. also clinical implications pregnancy monitoring, disease detection, sexual health management. In final step, manuscript provides future perspectives possibilities can be incorporated emerging field

Язык: Английский

Процитировано

4
Characterization of vaginal microbiomes in clinician-collected bacterial vaginosis diagnosed samples DOI Creative Commons
Hayden Brochu, Qimin Zhang, Kuncheng Song

и другие.

Microbiology Spectrum, Год журнала: 2025, Номер unknown

Опубликована: Фев. 25, 2025

ABSTRACT Bacterial vaginosis (BV) is a type of vaginal inflammation caused by bacterial overgrowth, upsetting the healthy microbiome vagina. Existing clinical testing for BV primarily based upon physical and microscopic examination secretions. Modern PCR-based tests target panels BV-associated microbes, such as Labcorp NuSwab test that targets Atopobium ( Fannyhessea ) vaginae , Megasphaera-1 Vaginosis Associated Bacterium (BVAB)−2 . Remnant clinician-collected swabs underwent DNA extraction 16S V3–V4 rRNA gene sequencing to profile microbes in addition those included test. Community state types (CSTs) were determined using most abundant taxon detected each sample. PCR results panel microbial compared against corresponding profiles. Metabolic pathway abundances characterized via metagenomic prediction from amplicon sequence variants (ASVs). 75 remnant yielded 492 unique ASVs, identifying 83 genera. microbe quantification was strongly concordant with P < 0.01). Samples CST-I (18 18, 100%), CST-II (three three, CST-III (15 17, 88%), CST-V (one one, 100%) largely categorized BV-negative panel, while CST-IV samples (28 36, 78%) BV-positive or BV-indeterminate. predicted metabolic signatures shared across multiple CSTs. These findings highlight robust sequencing-based accurate discrimination CSTs dominated distinct Lactobacilli expanded identification biomarkers. IMPORTANCE poses significant health burden women during reproductive years onward. Current diagnostics rely on either select evaluations technicians. Here, we sequenced profiles previously diagnosed better understand disruptions BV. We show can faithfully reproduce targeted diagnostic improve our knowledge This work highlights robust, agnostic classification scheme potential future development tools.

Язык: Английский

Процитировано

0
Fatty acid composition in the vaginal tract of cis-gender women: canary in coal mines for reproductive health? DOI Creative Commons
Karine Dufresne

Lipids in Health and Disease, Год журнала: 2025, Номер 24(1)

Опубликована: Март 3, 2025

Abstract The vaginal tract of cis-gender women reproductive age is inhabited by communities bacteria generally dominated one four Lactobacillus species. These are important for the health and favor better outcomes, including fertility, pregnancy leading to term protection against infections. Past studies have focused on role carbohydrates in balance communities, fatty acids has been underestimated. However, small- long-chain present few properties that, combination with sugar metabolism, affect outcomes or disease within communities. Herein, we explore origins as well their roles bacterial life cycle this environment. We also detail putative impact FAs S. aureus , etiologic agents aerobic vaginitis. Finally, discuss potential prevention therapy age.

Язык: Английский

Процитировано

0
Anti-oxidative activity of probiotics; focused on cardiovascular disease, cancer, aging, and obesity DOI
Batoul Kavyani, Somayeh Ahmadi, Edris Nabizadeh

и другие.

Microbial Pathogenesis, Год журнала: 2024, Номер 196, С. 107001 - 107001

Опубликована: Окт. 9, 2024

Язык: Английский

Процитировано

3
New Insights into Photobiomodulation of the Vaginal Microbiome—A Critical Review DOI Open Access
Fernanda Santos,

Carlota Anjinho Carvalhos,

Margarida Figueiredo-Dias

и другие.

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(17), С. 13507 - 13507

Опубликована: Авг. 31, 2023

The development of new technologies such as sequencing has greatly enhanced our understanding the human microbiome. interactions between microbiome and several diseases have been subject recent research. In-depth knowledge about vaginal (VMB) shown that dysbiosis is closely related to gynecologic obstetric disorders. To date, progress in treating or modulating VMB lagged far behind research efforts. Photobiomodulation (PBM) uses low levels light, usually red near-infrared, treat a diversity conditions. Several studies demonstrated PBM can control improve activity immune system. In years, increasing attention paid microbiome, mostly gut its connections with many diseases, metabolic disorders, obesity, cardiovascular autoimmunity, neurological applicability therapeutics studied, promising results. possible cellular molecular effects on constitute theoretical field starting take first steps. this review, we will discuss potential mechanisms photobiomodulation VMB.

Язык: Английский

Процитировано

7
Evaluating the antibacterial, antibiofilm, and anti-toxigenic effects of postbiotics from lactic acid bacteria on Clostridium difficile DOI Creative Commons
Mahdi Asghari Ozma, Hamideh Mahmoodzadeh Hosseini, Mohammad Hossein Ataee

и другие.

Iranian Journal of Microbiology, Год журнала: 2024, Номер unknown

Опубликована: Авг. 20, 2024

Background and Objectives: The most common cause of healthcare-associated diarrhea is Clostridium difficile infection (CDI), which causes severe recurring symptoms. increase antibiotic-resistant C. requires alternate treat- ments. Postbiotics, metabolites produced by probiotics, fight CDI owing to their antibacterial capabilities. This study aims evaluate the antibacterial, antibiofilm, anti-toxigenic potential postbiotics in combating CDI. Materials Methods: GC-MS evaluated from Bifidobacterium bifidum Lactobacillus plantarum. Disk diffusion broth microdilution determined inhibition zones MICs. Microtiter plates assessed antibiofilm activity. MTT assay anti-viability on HEK293. ELISA testing postbiotic detoxification toxins A B. Postbiotics were examined for tcdA tcdB genes expression using real-time PCR. Results: identified L. plantarum compounds glycolic acid (7.2%) butyric (13.57%). displayed 13 10 mm 2.5 5 mg/ml MICs against difficile. reduced biofilm at 1.25 49% 31%. showed both had little influence cell viability, was over 80%. power revealed that decreased toxin B production more effectively than plantarum, also related reduction statistically significant (p < 0.05). Conclusion: Postbiotics' ability inhibit bacterial growth, disruption, makes them a promising adjunctive treatment good solution pathogens' antibiotic resistance.

Язык: Английский

Процитировано

2