GATA1‐activated lncRNA OIP5AS1 and GAS5 promote pyroptosis to exacerbate asthma through regulating miR‐136‐5p/LIFR axis DOI
S. J. Zhao,

Sini Huang,

Yawei Wu

и другие.

The FASEB Journal, Год журнала: 2024, Номер 38(22)

Опубликована: Ноя. 13, 2024

Abstract Pyroptosis plays a pivotal role in airway epithelial inflammation during the progression of asthma. This study aimed to explore influence and mechanisms opa‐interacting protein 5 antisense RNA1 (OIP5‐AS1) growth arrest‐specific transcript (GAS5) on pyroptosis asthmatic models. was induced Dermatophagoides pteronyssinus 1 (Der p1)‐exposed 16HBE cells ovalbumin (OVA)‐challenged rats. Subsequently, its related molecular were investigated. Our results indicated that GATA1, OIP5‐AS1, GAS5, LIFR upregulated, while miR‐136‐5p downregulated patients experimental models OIP5‐AS1/GAS5 knockdown repressed NLRP3 inflammasome‐mediated cells. Mechanistically, sponged enhance expression subsequently activated NF‐κB pathway. or LIFR‐mediated induction abrogated by mimics inhibitors (BAY11‐7082). Finally, GATA1 transcriptionally trigger pyroptosis, thereby driving asthma vivo vitro. In conclusion, promoted via modulation miR‐136‐5p/LIFR/NF‐κB axis consequently resulted may provide novel therapeutic strategies for

Язык: Английский

Plant Secondary Metabolites as Modulators of Mitochondrial Health: An Overview of Their Anti-Oxidant, Anti-Apoptotic, and Mitophagic Mechanisms DOI Open Access

Julia Anchimowicz,

Piotr Zielonka, Sławomir Jakieła

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(1), С. 380 - 380

Опубликована: Янв. 4, 2025

Plant secondary metabolites (PSMs) are a diverse group of bioactive compounds, including flavonoids, polyphenols, saponins, and terpenoids, which have been recognised for their critical role in modulating cellular functions. This review provides comprehensive analysis the effects PSMs on mitochondrial health, with particular emphasis therapeutic potential. Emerging evidence shows that these improve function by reducing oxidative stress, promoting biogenesis, regulating key processes such as apoptosis mitophagy. Mitochondrial dysfunction, hallmark many pathologies, neurodegenerative disorders, cardiovascular diseases, metabolic syndrome, has shown to benefit from protective PSMs. Recent studies show can dynamics, stabilise membranes, enhance bioenergetics, offering significant promise prevention treatment mitochondrial-related diseases. The molecular mechanisms underlying effects, modulation signalling pathways direct interactions proteins, discussed. integration into strategies is highlighted promising avenue improving efficacy while minimising side commonly associated synthetic drugs. also highlights need future research elucidate specific roles individual synergistic within complex plant matrices, may further optimise utility. Overall, this work valuable insights health potential natural agents targeting dysfunction.

Язык: Английский

Процитировано

3

Neurobiological potential of astragaloside IV and prospects for its use in the treatment of Alzheimer’s disease DOI
М. А. Berezutsky, Н. А. Дурнова, M. N. Kurchatova

и другие.

S S Korsakov Journal of Neurology and Psychiatry, Год журнала: 2025, Номер 125(2), С. 7 - 7

Опубликована: Март 6, 2025

The review presents an analysis of experimental data on the study neurobiological effects astragaloside IV, which can be used in treatment Alzheimer's disease. Astragaloside IV is a cycloartan triterpene saponin, found roots membranous milk vetch (Astragalus membranaceus (Fisch. ex Link) Bunge) and has very wide range pharmacological activity. In recent years, this compound attracted attention due to its diverse effects. Studies have shown ability modulate microglial protective effect saponin neurons from glutamate-induced neurotoxicity been demonstrated. PC12 cells, able resolve various types mitochondrial dysfunction inhibit endoplasmic reticulum stress. This also PPARγ agonist. vivo experiments that test substance effectively protects synapses as well improves cognitive functions, including memory learning. It concluded apparently, may future multi-purpose complex therapy for

Язык: Английский

Процитировано

0

Polysaccharides, saponins and flavonoids in Astragali Radix: a review of phytochemistry and pharmacology for future therapeutic applications DOI
Xiaolin Huang,

Ziyuan Guo,

Zihan Wang

и другие.

Phytochemistry Reviews, Год журнала: 2025, Номер unknown

Опубликована: Апрель 9, 2025

Язык: Английский

Процитировано

0

Astragaloside IV protects against autoimmune myasthenia gravis in rats via regulation of mitophagy and apoptosis DOI Creative Commons
Jingjing Zhang,

Jiayan Huang,

Jinlian Lan

и другие.

Molecular Medicine Reports, Год журнала: 2024, Номер 30(1)

Опубликована: Май 24, 2024

Astragaloside IV (AS‑IV) has various pharmacological effects, including antioxidant and immunoregulatory properties, which can improve myasthenia gravis (MG) symptoms. However, the potential mechanism underlying effects of AS‑IV on MG remains to be elucidated. The present study aimed investigate whether a therapeutic effect its action. By subcutaneously immunizing rats with R97‑116 peptide, an experimental autoimmune (EA) rat model was established. (40 or 80 mg/kg/day) treatment then applied for 28 days after modeling. results demonstrated that significantly ameliorated weight loss, Lennon score pathological changes in gastrocnemius muscle EAMG compared group. Additionally, levels acetylcholine receptor antibody (AChR‑Ab) were decreased, whereas mitochondrial function [ATPase cytochrome c (Cyt‑C) oxidase activities] ultrastructure improved treated rats. Moreover, mRNA protein expression phosphatase tensin homolog‑induced putative kinase 1, Parkin, LC3II Bcl‑2, key signaling molecules mitophagy apoptosis, upregulated, p62, Cyt‑C, Bax, caspase 3 9 downregulated following intervention. In conclusion, may protect against by modulating apoptosis. These findings indicated provided novel insights into strategies MG.

Язык: Английский

Процитировано

2

Astragalus membranaceus, Nigella sativa, and Perilla frutescens as Immunomodulators—Molecular Mechanisms and Clinical Effectiveness in Allergic Diseases DOI Creative Commons

Maja Bival Štefan

Current Issues in Molecular Biology, Год журнала: 2024, Номер 46(8), С. 9016 - 9032

Опубликована: Авг. 17, 2024

Plants are the source of numerous remedies in modern medicine, and some them have been studied due to their potential immunomodulatory activity. Astragalus membranaceus Fisch. ex Bunge (A. membranaceus), Nigella sativa L. (N. sativa), Perilla frutescens (L.) Britton (P. frutescens) plant species used traditional medicine for treatment various diseases. Their act as immunomodulatory, anti-inflammatory, anti-allergic agents makes interesting investigating clinical alleviating symptoms allergic Allergy affects a large number people; according sources more than 30% world population suffer from type reaction, with pollen allergy most common type. Treatment is usually pharmacological may not be completely effective or side effects. Thus, we seeking mostly medicinal plants, promising symptoms. A literature overview was conducted employing databases such Scopus, PubMed, Web Science, Springer, Google Scholar. This manuscript summarizes recent vivo preclinical studies on three activity, provides comparison effects, underlines application practice. The obtained results confirmed efficacy studies, but also emphasize problem phytochemical characterization difference between tested doses. More trials standardized protocols (defined active molecules, dosage, effects) required obtain safe herbal drugs.

Язык: Английский

Процитировано

2

Separation of astragaloside IV from Astragalus membranaceus based on high‐speed countercurrent chromatography in continuous injection mode DOI

Y. Zhao,

Qian‐Xia Su,

Ningli Wang

и другие.

Phytochemical Analysis, Год журнала: 2024, Номер unknown

Опубликована: Авг. 26, 2024

Abstract Introduction Astragaloside IV (AS‐IV) is an index for the quality evaluation of traditional Chinese medicine Astragalus and important material basis to exert its medicinal effects, it difficult obtain a single AS‐IV by ordinary separation methods. Objective To find new isolation method that can prepare quickly efficiently. Methodology was isolated from membranaceus extract high‐speed countercurrent chromatography using two‐phase solvent system consisting ethyl acetate/ n ‐butanol/water (4.2:0.8:5, v/v) at speed 950 rpm flow rate 2 mL/min one chromatographic sequential injection models developed during previous study. Results Compared with common separation, this increased volume yield 4‐fold 4.47‐fold, respectively, only about 1.2‐fold increase in consumption time, purity basically not reduced, 55.9 mg AS‐IV, 96.95%, finally prepared 400 crude 240 min. Conclusion The continuous mode able successfully large amount high time.

Язык: Английский

Процитировано

0

GATA1‐activated lncRNA OIP5AS1 and GAS5 promote pyroptosis to exacerbate asthma through regulating miR‐136‐5p/LIFR axis DOI
S. J. Zhao,

Sini Huang,

Yawei Wu

и другие.

The FASEB Journal, Год журнала: 2024, Номер 38(22)

Опубликована: Ноя. 13, 2024

Abstract Pyroptosis plays a pivotal role in airway epithelial inflammation during the progression of asthma. This study aimed to explore influence and mechanisms opa‐interacting protein 5 antisense RNA1 (OIP5‐AS1) growth arrest‐specific transcript (GAS5) on pyroptosis asthmatic models. was induced Dermatophagoides pteronyssinus 1 (Der p1)‐exposed 16HBE cells ovalbumin (OVA)‐challenged rats. Subsequently, its related molecular were investigated. Our results indicated that GATA1, OIP5‐AS1, GAS5, LIFR upregulated, while miR‐136‐5p downregulated patients experimental models OIP5‐AS1/GAS5 knockdown repressed NLRP3 inflammasome‐mediated cells. Mechanistically, sponged enhance expression subsequently activated NF‐κB pathway. or LIFR‐mediated induction abrogated by mimics inhibitors (BAY11‐7082). Finally, GATA1 transcriptionally trigger pyroptosis, thereby driving asthma vivo vitro. In conclusion, promoted via modulation miR‐136‐5p/LIFR/NF‐κB axis consequently resulted may provide novel therapeutic strategies for

Язык: Английский

Процитировано

0