Investigating mechanisms of apoptosis of humic acid on Leishmania tropica: An in vitro and in silico approach.
Heliyon,
Год журнала:
2025,
Номер
unknown, С. e43017 - e43017
Опубликована: Март 1, 2025
Highlights•Humic
acid
(HA)
demonstrated
significant
potential
to
trigger
programmed
cell
death
in
Leishmania
tropica-infected
macrophages.
This
effect
was
observed
through
the
probable
modulation
of
both
intrinsic
and
extrinsic
apoptotic
pathways.•HA
combined
with
meglumine
antimoniate
(MA),
revealed
enhanced
anti-leishmanial
activity.
The
combination
treatment
exhibited
a
nearly
additive
effect,
leading
greater
efficacy
diminishing
viability
L.
tropica
parasites,
particularly
amastigote
form,
compared
either
alone.•Molecular
docking
assay
indicated
that
HA
binds
beneficially
key
proteins,
Bax
Bcl2.•The
study
found
also
boosts
antioxidative
activities
intra-macrophage
amastigotes.
suggests
may
eliminate
oxidative
stress,
which
is
often
elevated
during
leishmaniasis,
thereby
improving
overall
therapeutic
profile
against
infection.•Generally,
findings
indicate
humic
could
serve
as
promising
agent
for
treating
especially
when
used
conventional
treatments
like
antimoniate.
emphasizes
need
further
exploration
HA's
mechanisms
its
application
animal
studies.AbstractLeishmaniasis,
parasitic
neglected
tropical
disease,
contributes
remarkably
global
morbidity
mortality.
Current
are
facing
multiple
limitations,
including
side
effects,
drug
resistance,
high
costs.
Consequently,
there
pressing
develop
efficacious,
widely
accessible,
lower-toxic
compounds.
Humic
has
previously
shown
potential,
targeting
apoptosis-related
pathways
cancerous
infected
cells.
research
structured
investigate
activity
alone
(MA)
on
infection.
We
employed
molecular
predict
functional
residues
structural
pockets
Bcl2
proteins.
Then,
we
conducted
vitro
assessments,
protein
expression
analysis,
immunocytochemistry
profiles
antioxidant
mediators
treated
human
Molecular
binding
interactions
between
Furthermore,
more
MA
by
inducing
action,
specifically
apoptosis
macrophages
amastigotes
modulating
level
proteins
involved
internal
external
assays.
Notably,
our
results
displayed
can
promote
amastogites
tropica.
Thus,
these
vivo
ex
studies,
antimonials.Graphical
abstract
Язык: Английский
Encapsulation of Citrus sinensis essential oil and R-limonene in lipid nanocarriers: A potential strategy for the treatment of leishmaniasis
International Journal of Pharmaceutics,
Год журнала:
2024,
Номер
662, С. 124464 - 124464
Опубликована: Июль 20, 2024
Язык: Английский
Immunomodulatory activity of bamboo biosilica from Dendrocalamus longispathus Kurz. – Metabolomics integrated network pharmacology approach
Phytomedicine,
Год журнала:
2025,
Номер
143, С. 156797 - 156797
Опубликована: Апрель 26, 2025
Язык: Английский
Advance in Nrf2 Signaling Pathway in Leishmaniasis
Biomedicines,
Год журнала:
2024,
Номер
12(11), С. 2525 - 2525
Опубликована: Ноя. 4, 2024
One
of
the
main
components
innate
defense
against
invasive
parasites
is
oxidative
stress,
which
brought
on
by
reactive
oxygen
species
(ROS).
On
other
hand,
stressors
serve
two
purposes:
free
radicals
aid
in
elimination
pathogens,
but
they
can
also
set
off
inflammation,
leads
to
tissue
damage.
Nuclear
factor
erythroid
2-related
2
(Nrf2)
a
transcription
that
controls
expression
numerous
genes
involved
body's
stress
aging,
damage,
and
pathological
consequences.
From
cutaneous
visceral
forms,
Язык: Английский
Identification of immunostimulatory activities and active compounds from sequentially extracted fractions of rhizosphere fungal fermentation broth of Atractylodes macrocephala Koidz. rhizomes
Yuxin Xie,
Na Lin,
Pingping Song
и другие.
Frontiers in Pharmacology,
Год журнала:
2024,
Номер
15
Опубликована: Дек. 20, 2024
Pharmacological
studies
have
shown
that
the
rhizome
of
Atractylodes
macrocephala
Koidz.
(Compositae),
commonly
known
as
atractylodes
(AMR),
can
modulate
immunity.
Nevertheless,
its
resources
been
largely
depleted,
and
pharmacological
activity
artificial
AMR
is
relatively
modest.
We
hypothesized
fermented
crude
extracts
rhizosphere
fungi
would
similar
immunomodulatory
effects
since
metabolites
generated
by
these
are
to
those
host
plant
given
their
long-term
synergistic
evolution.
Язык: Английский