Platelet-Rich Plasma in Diabetic Foot Ulcer Healing: Contemplating the Facts DOI Open Access
Jacob A. Smith, Vikrant Rai

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(23), С. 12864 - 12864

Опубликована: Ноя. 29, 2024

Diabetic foot ulcers (DFUs), debilitating complication of diabetes, often lead to amputation even in the presence current advanced treatment for DFUs. Platelet-rich plasma (PRP) containing growth factors and other proteins has been suggested as a potent therapeutic promoting DFU healing. PRP is safe effective improving healing rate, decreasing time, making chronic wounds viable treatment. Though healing, there are inconsistencies clinical outcomes. These varying results may be due various concentrations being used. Most studies report dosage timing, but none have reported concentration factors. This important, can vary significantly with each preparation directly impact outcome. critical review discusses limiting issues related therapy future directives. A systematic search PubMed Google Scholar was performed keywords including diabetic ulcer, ulcer platelet-rich plasma, treatment, limitations efficacy, alone or combination, articles. The articles describing use were included. existing literature suggests that larger trials needed improve There need consider multiple role epigenetics, lifestyle modification, percentage composition constituent PRP.

Язык: Английский

Macrophage plasticity: signaling pathways, tissue repair, and regeneration DOI Creative Commons
Lingfeng Yan, Jue Wang,

Xin Cai

и другие.

MedComm, Год журнала: 2024, Номер 5(8)

Опубликована: Авг. 1, 2024

Abstract Macrophages are versatile immune cells with remarkable plasticity, enabling them to adapt diverse tissue microenvironments and perform various functions. Traditionally categorized into classically activated (M1) alternatively (M2) phenotypes, recent advances have revealed a spectrum of macrophage activation states that extend beyond this dichotomy. The complex interplay signaling pathways, transcriptional regulators, epigenetic modifications orchestrates polarization, allowing respond stimuli dynamically. Here, we provide comprehensive overview the cascades governing focusing on roles Toll‐like receptors, signal transducer activator transcription proteins, nuclear microRNAs. We also discuss emerging concepts metabolic reprogramming trained immunity, contributing their functional adaptability. Macrophage plasticity plays pivotal role in repair regeneration, macrophages coordinating inflammation, angiogenesis, matrix remodeling restore homeostasis. By harnessing potential novel therapeutic strategies targeting polarization could be developed for diseases, including chronic wounds, fibrotic disorders, inflammatory conditions. Ultimately, deeper understanding molecular mechanisms underpinning will pave way innovative regenerative medicine engineering approaches.

Язык: Английский

Процитировано

43
Glycolipid Metabolic Disorders, Metainflammation, Oxidative Stress, and Cardiovascular Diseases: Unraveling Pathways DOI Creative Commons

Enzo Pereira de Lima,

Renato Cesar Moretti,

Karina Torres Pomini

и другие.

Biology, Год журнала: 2024, Номер 13(7), С. 519 - 519

Опубликована: Июль 12, 2024

Glycolipid metabolic disorders (GLMDs) are various resulting from dysregulation in glycolipid levels, consequently leading to an increased risk of obesity, diabetes, liver dysfunction, neuromuscular complications, and cardiorenal vascular diseases (CRVDs). In patients with GLMDs, excess caloric intake a lack physical activity may contribute oxidative stress (OxS) systemic inflammation. This study aimed review the connection between GLMD, OxS, metainflammation, onset CRVD. GLMD is due causing dysfunction synthesis, breakdown, absorption glucose lipids body, excessive ectopic accumulation these molecules. mainly neuroendocrine dysregulation, insulin resistance, metainflammation. many inflammatory markers defense cells play vital role related tissues organs, such as blood vessels, pancreatic islets, liver, muscle, kidneys, adipocytes, promoting lesions that affect interconnected organs through their signaling pathways. Advanced glycation end products, ATP-binding cassette transporter 1, Glucagon-like peptide-1, Toll-like receptor-4, sphingosine-1-phosphate (S1P) crucial since they glucolipid metabolism. The consequences this system organ damage morbidity mortality.

Язык: Английский

Процитировано

25
Sandwich‐Structured Nanofiber Dressings Containing MgB2 and Metformin Hydrochloride With ROS Scavenging and Antibacterial Properties for Wound Healing in Diabetic Infections DOI

Yueguang Fang,

Lanling Xiu,

Dingwen Xiao

и другие.

Advanced Healthcare Materials, Год журнала: 2024, Номер unknown

Опубликована: Сен. 5, 2024

Abstract The treatment of chronic diabetic wounds is a major challenge due to oxidative stress, persistent hyperglycemia, and susceptibility bacterial infection. In this study, multifunctional sandwich‐structured nanofiber dressings (SNDs) are prepared via electrospinning. SNDs consisted an outer layer hydrophobic polylactic acid (PLA) fibers encapsulating MgB 2 nanosheets (MgB NSs), middle PLA polyvinylpyrrolidone (PVP) the NSs metformin hydrochloride complex ‐Met), inner water‐soluble PVP ‐Met. Because their special sandwich structure, have high photothermal conversion efficiency (24.13%) cycle performance. also exhibit effect, bacteria‐targeting effect , electrostatic attraction ability (Met), strong antibacterial activity against Escherichia coli ( E. ) methicillin‐resistant Staphylococcus aureus (MRSA). can eliminate intracellular reactive oxygen species (ROS) by regulating hydrogen release from . addition, good biocompatibility, effectively inhibit inflammatory factor Interleukin‐6 (IL‐6), promote granulation tissue formation, collagen deposition, wound healing. These findings offer promising approach for clinical wounds.

Язык: Английский

Процитировано

4
Crosstalk Between H-Type Vascular Endothelial Cells and Macrophages: A Potential Regulator of Bone Homeostasis DOI Creative Commons

Jiaxuan Fan,

Yaohui Xie,

Dayin Liu

и другие.

Journal of Inflammation Research, Год журнала: 2025, Номер Volume 18, С. 2743 - 2765

Опубликована: Фев. 1, 2025

The crosstalk between H-type endothelial cells (ECs) and macrophages is critical for maintaining angiogenesis osteogenesis in bone homeostasis. As core components of type H vessels, ECs respond to various pro-angiogenic signals, forming specialized vascular structures characterized by high expression platelet-endothelial cell adhesion molecule-1 (CD31) mucin (EMCN), thereby facilitating angiogenesis-osteogenesis coupling during formation. Macrophages, as key immune the perivascular region, are primarily classified into classically activated pro-inflammatory M1 phenotype selectively anti-inflammatory M2 phenotype, performing dual functions regulating local tissue homeostasis innate immunity. In recent years, complex vessel has garnered significant interest context bone-related diseases. Orderly regulation immunity provides a new direction preventing metabolic disorders such osteoporosis osteoarthritis. However, their interactions remain insufficiently understood, with limited clinical data available. This review comprehensively examines intricate diverse phenotypes, Insights signaling pathways that regulate crosstalk, focusing on roles osteogenesis. Furthermore, discusses interventions targeting this challenges remain. These insights may offer perspectives provide theoretical foundation developing novel therapeutic strategies.

Язык: Английский

Процитировано

0
Combining Network Pharmacology, Molecular Docking, Molecular Dynamics Simulation, and Experimental Validation to Uncover the Efficacy and Mechanisms of Si-Miao-Yong-An Decoction in Diabetic Wound Healing DOI Creative Commons

Shujuan Zhang,

Yiming Shao,

Jin Ranran

и другие.

Journal of Inflammation Research, Год журнала: 2025, Номер Volume 18, С. 4087 - 4101

Опубликована: Март 1, 2025

Si-Miao-Yong-An (SMYA) Decoction, a traditional Chinese herbal mixture, shows promise for managing diabetic complications. Up to this point, no reports have explored the effects of SMYA on wounds or underlying mechanisms. This study aimed investigate therapeutic potential in promoting wound healing and elucidate molecular The were evaluated db/db mice by measuring closure rates histological characteristics, including epidermal thickness collagen deposition. Network pharmacology was utilized identify active ingredients corresponding targets SMYA, followed validation through docking dynamics simulations. KEGG GO enrichment analyses conducted relevant biological processes pathways. In vitro studies involving high-glucose-treated HUVECs assessed SMYA-containing serum cellular migration angiogenesis. Finally, expression inflammatory factors RAGE tissue detected qRT-PCR. significantly accelerated mice, as evidenced improved thickness, morphology, identified 140 overlapping genes involved angiogenesis inflammation, with AGE-RAGE signaling pathway playing central role. Molecular simulations revealed strong binding stability quercetin kaempferol inflammation-related hub targets, IL-6, TNF, IL-1β. vitro, alleviated high-glucose-induced impairments HUVEC Furthermore, qRT-PCR analysis showed that downregulated Tnf, Il1b, Il6, Rage tissues, supporting its anti-inflammatory effect. promotes modulating microenvironment inhibiting pathway. These findings provide robust evidence SMYA's lay foundation future clinical application treating wounds.

Язык: Английский

Процитировано

0
Rapamycin-Induced Small Extracellular Vesicles under GelMA Scaffolds Facilitate Diabetic Wound Repair through Accelerating Angiogenesis and Alleviating Macrophage-mediated Inflammation via PI3K/Akt Signaling Pathway DOI Creative Commons
Yalu Zhang,

Zekun Wang,

Liang Wu

и другие.

Materials Today Bio, Год журнала: 2025, Номер 32, С. 101846 - 101846

Опубликована: Май 6, 2025

Язык: Английский

Процитировано

0
Progress of microneedle targeted modulation technology in the reconstruction of immune microenvironment in diabetic wounds DOI Creative Commons

Shunsheng Wang,

Wei Cheng,

Xue Wang

и другие.

European journal of medical research, Год журнала: 2025, Номер 30(1)

Опубликована: Май 20, 2025

Wound healing in diabetic patients is mainly hindered by a combination of long-term glycosylation, persistent inflammatory response, and immunosuppressive state. The interaction these factors not only results considerable prolongation the wound process but also elevates likelihood recurrent ulcer development, profoundly affecting patients' quality life. Traditional treatments, including surgical debridement, anti-infection, dressing application, vascular intervention, glycaemic control, can relieve some symptoms. However, they are often ineffective addressing underlying cause impaired healing. It concern that importance immune microenvironment has yet been fully appreciated investigated, homeostasis crucial for promoting cell proliferation, angiogenesis, tissue repair. this dysregulated This paper reviews key leading to dysregulation microenvironment, dysfunction, abnormal cytokine expression, disruption signalling pathways, introduces an innovative silicone-based microneedle drug delivery method, which takes advantage microneedle's precise targeting highly efficient loading capacity deliver drugs with immunomodulatory functions directly sustained manner, activate corresponding promote polarization M1 macrophages into M2 phenotype, stimulate neovascularization, providing low pro-angiogenic healing, provides new therapeutic idea means

Язык: Английский

Процитировано

0
Wound Healing in the Modern Era: Emerging Research, Biomedical Advances, and Transformative Clinical Approaches DOI

Subhasri Bogadi,

Md Uddin,

M. Habibur Rahman

и другие.

Journal of Drug Delivery Science and Technology, Год журнала: 2025, Номер unknown, С. 107058 - 107058

Опубликована: Май 1, 2025

Язык: Английский

Процитировано

0
Platelet-Rich Plasma in Diabetic Foot Ulcer Healing: Contemplating the Facts DOI Open Access
Jacob A. Smith, Vikrant Rai

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(23), С. 12864 - 12864

Опубликована: Ноя. 29, 2024

Diabetic foot ulcers (DFUs), debilitating complication of diabetes, often lead to amputation even in the presence current advanced treatment for DFUs. Platelet-rich plasma (PRP) containing growth factors and other proteins has been suggested as a potent therapeutic promoting DFU healing. PRP is safe effective improving healing rate, decreasing time, making chronic wounds viable treatment. Though healing, there are inconsistencies clinical outcomes. These varying results may be due various concentrations being used. Most studies report dosage timing, but none have reported concentration factors. This important, can vary significantly with each preparation directly impact outcome. critical review discusses limiting issues related therapy future directives. A systematic search PubMed Google Scholar was performed keywords including diabetic ulcer, ulcer platelet-rich plasma, treatment, limitations efficacy, alone or combination, articles. The articles describing use were included. existing literature suggests that larger trials needed improve There need consider multiple role epigenetics, lifestyle modification, percentage composition constituent PRP.

Язык: Английский

Процитировано

2