PLoS ONE,
Год журнала:
2024,
Номер
19(5), С. e0302496 - e0302496
Опубликована: Май 6, 2024
Adult
mosquitoes
require
regular
sugar
meals,
including
nectar,
to
survive
in
natural
habitats.
Both
males
and
females
locate
potential
sources
using
sensory
proteins
called
odorant
receptors
(ORs)
activated
by
plant
volatiles
orient
toward
flowers
or
honeydew.
The
yellow
fever
mosquito,
Aedes
aegypti
(Linnaeus,
1762),
possesses
a
large
gene
family
of
ORs,
many
which
are
likely
detect
floral
odors.
In
this
study,
we
have
uncovered
ligand-receptor
pairings
for
suite
ORs
panel
environmentally
relevant,
plant-derived
volatile
chemicals
heterologous
expression
system.
Our
results
support
the
hypothesis
that
these
odors
mediate
responses
mosquito's
central
nervous
system,
thereby
influencing
appetitive
aversive
behaviors.
Further,
well
conserved
other
mosquitoes,
suggesting
they
function
similarly
diverse
species.
This
information
can
be
used
assess
mosquito
foraging
behavior
develop
novel
control
strategies,
especially
those
incorporate
bait-and-kill
technologies.
Frontiers in Physiology,
Год журнала:
2023,
Номер
14
Опубликована: Июль 14, 2023
A
sophisticated
and
sensitive
olfactory
system
plays
a
vital
role
in
the
survival
reproduction
of
insects.
Chemosensory
receptors
are
indispensable
for
molecular
recognition
discrimination
semiochemicals.
Riptortus
pedestris
is
notorious
pest
legume
plants,
resulting
yield
losses
quality
decreases
soybeans.
It
well
accepted
that
R.
highly
relies
on
its
detecting
aggregation
pheromones,
host
volatiles,
pesticides;
however,
little
research
focused
chemosensory
receptors.
In
present
study,
we
identified
237
odorant
(ORs),
42
gustatory
(GRs),
31
ionotropic
(IRs)
from
reported
genome
pedestris,
analyzed
their
phylogenetic
relationship
with
other
hemipteran
species.
Through
results
RNA-seq
real-time
quantitative
PCR
(qRT-PCR),
found
RpedORs
displayed
different
expression
levels
antennae
at
development
stages.
To
further
verify
function
receptor
co-receptor
(Orco),
an
obligate
unique
insect
OR,
silenced
RpedOrco
by
RNA
interference
(RNAi)
method.
The
showed
silencing
could
significantly
impair
response
to
pheromone
indicating
essential
detection.
Our
can
provide
theoretical
foundations
revealing
mechanism
help
explore
develop
novel
olfactory-based
agents
against
this
pest.
Cell Reports Methods,
Год журнала:
2024,
Номер
4(2), С. 100714 - 100714
Опубликована: Фев. 1, 2024
Anopheles
gambiae
uses
its
sense
of
smell
to
hunt
humans.
We
report
a
two-step
method
yielding
cell-type-specific
driver
lines
for
enhanced
neuroanatomical
and
functional
studies
olfactory
system.
first
integrated
driver-responder-marker
(DRM)
system
cassette
consisting
linked
T2A-QF2
driver,
QUAS-GFP
responder,
gut-specific
transgenesis
marker
into
four
chemoreceptor
genes
(Ir25a,
Ir76b,
Gr22,
orco)
using
CRISPR-Cas9-mediated
homology-directed
repair.
The
DRM
facilitated
rapid
selection
in-frame
integrations
via
screening
GFP+
sensory
neurons
(OSNs)
in
G
PLoS ONE,
Год журнала:
2024,
Номер
19(5), С. e0302496 - e0302496
Опубликована: Май 6, 2024
Adult
mosquitoes
require
regular
sugar
meals,
including
nectar,
to
survive
in
natural
habitats.
Both
males
and
females
locate
potential
sources
using
sensory
proteins
called
odorant
receptors
(ORs)
activated
by
plant
volatiles
orient
toward
flowers
or
honeydew.
The
yellow
fever
mosquito,
Aedes
aegypti
(Linnaeus,
1762),
possesses
a
large
gene
family
of
ORs,
many
which
are
likely
detect
floral
odors.
In
this
study,
we
have
uncovered
ligand-receptor
pairings
for
suite
ORs
panel
environmentally
relevant,
plant-derived
volatile
chemicals
heterologous
expression
system.
Our
results
support
the
hypothesis
that
these
odors
mediate
responses
mosquito's
central
nervous
system,
thereby
influencing
appetitive
aversive
behaviors.
Further,
well
conserved
other
mosquitoes,
suggesting
they
function
similarly
diverse
species.
This
information
can
be
used
assess
mosquito
foraging
behavior
develop
novel
control
strategies,
especially
those
incorporate
bait-and-kill
technologies.
