Identification of a Soybean Volatile Attractive for Riptortus pedestris Using Reverse Chemical Ecology Approach DOI
Xiaotong Zhang,

Xuan-Pu Luan,

Jiahang Wei

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 27, 2024

The bean bug Riptortus pedestris is a major soybean pest and cause of the stay-green symptoms. However, molecular mechanisms underlying its olfaction-mediated host-seeking behavior remain unclear. In this study, we compared antennae transcriptomes starved nonstarved adult R. pedestris, identifying four differentially expressed odorant receptor (OR) genes. Among these, RpedOR13 showed strong response to host volatile 2-phenylethanol (2-PE) in Xenopus oocyte assays, while electroantennography behavioral tests confirmed 2-PE as an effective attractant. Next, phylogenetic analysis identified RpedOR72b paralog RpedOR13, with subsequent assays confirming specific 2-PE. Additionally, RNA interference experiments highlighted crucial role detecting Taken together, these findings provide new insights into highlight successful application reverse chemical ecology OR-based screening bioactive compounds.

Язык: Английский

Evaluation of Reference Genes for Quantitative Real-Time PCR Analysis in the Bean Bug, Riptortus pedestris (Hemiptera: Alydidae) DOI Creative Commons
L. Wang, Qing‐Yu Liu,

Pei Guo

и другие.

Insects, Год журнала: 2023, Номер 14(12), С. 960 - 960

Опубликована: Дек. 18, 2023

Quantitative real-time PCR (qRT-PCR) is widely accepted as a precise and convenient method for quantitatively analyzing the expression of functional genes. The data normalization strongly depends upon stable reference bean bug, Riptortus pedestris (Hemiptera: Alydidae), significant pest leguminous crops broadly distributed across Southeast Asia. In this study, total 16 candidate genes (RPL32, RPS23, SDHA, UBQ, UCCR, GST, TATA-box, HSP70, GAPDH, RPL7A, SOD, RPS3, Actin, α-tubulin, AK, EF1) were carefully chosen in R. pedestris, their levels assessed various conditions, including different developmental stages, diverse tissues, temperature treatments, adult age, molting time, mating status. Following this, stability these was evaluated using four algorithms (ΔCt, GeNorm, NormFinder, BestKeeper). Ultimately, comprehensive rankings determined online tool RefFinder. Our results demonstrate that gene qRT-PCR analysis contingent specific experimental conditions. RPL7A EF1 are optimal stages. Furthermore, α-tubulin exhibit most tissues. RPL32 age. For nymph SOD display expression. RPS23 identified suitable monitoring Lastly, we verified practicability evaluating odorant-binding protein 37 (RpedOBP37) cytochrome P450 6a2 (RpedCYP6) throughout These findings addition to studies on serving fundamental groundwork future investigations well other organisms.

Язык: Английский

Процитировано

6
RpedOBP1 plays key roles in aggregation pheromones reception of the Riptortus pedestris DOI
Yongzhi Zhong, Rui Tang, Lulu Lin

и другие.

Pesticide Biochemistry and Physiology, Год журнала: 2024, Номер 204, С. 106073 - 106073

Опубликована: Авг. 5, 2024

Язык: Английский

Процитировано

1
Identification and expression profiles of olfactory-related genes in the antennal transcriptome of Graphosoma rubrolineatum (Hemiptera: Pentatomidae) DOI Creative Commons

Shibao Guo,

Panjing Liu,

Yin Tang

и другие.

PLoS ONE, Год журнала: 2024, Номер 19(8), С. e0306986 - e0306986

Опубликована: Авг. 6, 2024

Graphosoma rubrolineatum (Hemiptera: Pentatomidae) is an important pest of vegetables and herbs (e.g., Umbelliferae Cruciferae) in China, Siberia, Korea, Japan. Insects are highly dependent on their olfactory system to detect odorants. However, no molecular-mediated genes G . have yet been identified. In this study, we first established the antennal transcriptome identified 189 candidate genes, including 31 odorant-binding proteins (OBPs), 15 chemosensory (CSPs), four sensory neuron membrane (SNMPs),94 odorant receptors (ORs), 23 ionotropic (IRs), 22 gustatory (GRs). Additionally, phylogenetic trees were constructed for between other hemipteran insects. We also detected expression profiles ten OBPs, five CSPs, two SNMPs, ORs, IRs, GRs by real-time quantitative PCR. The results revealed that most ( GrubOBP1/11/31 , GrubCSP3/8 GrubSNMP1a/1b GrubOrco/OR9/11/13 GrubGR1/4/22 GrubIR25/75h/76b/GluR1 ) expressed antennae, GrubOBP13/31 rubCSP4/11/12 legs, while GrubOBP20 GrubGR19 wings. Our will enrich gene inventory provide further insight into molecular mechanisms

Язык: Английский

Процитировано

0
Identification of a Soybean Volatile Attractive for Riptortus pedestris Using Reverse Chemical Ecology Approach DOI
Xiaotong Zhang,

Xuan-Pu Luan,

Jiahang Wei

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 27, 2024

The bean bug Riptortus pedestris is a major soybean pest and cause of the stay-green symptoms. However, molecular mechanisms underlying its olfaction-mediated host-seeking behavior remain unclear. In this study, we compared antennae transcriptomes starved nonstarved adult R. pedestris, identifying four differentially expressed odorant receptor (OR) genes. Among these, RpedOR13 showed strong response to host volatile 2-phenylethanol (2-PE) in Xenopus oocyte assays, while electroantennography behavioral tests confirmed 2-PE as an effective attractant. Next, phylogenetic analysis identified RpedOR72b paralog RpedOR13, with subsequent assays confirming specific 2-PE. Additionally, RNA interference experiments highlighted crucial role detecting Taken together, these findings provide new insights into highlight successful application reverse chemical ecology OR-based screening bioactive compounds.

Язык: Английский

Процитировано

0