Identification of Δ9 and Δ11 Desaturases Involved in Sex Pheromone Biosynthesis in Mythimna loreyi (Lepidoptera: Noctuidae)
Journal of Agricultural and Food Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 1, 2025
In
moths,
sex
pheromones
are
synthesized
in
pheromone
glands
(PGs)
by
a
variety
of
enzymes.
Desaturases
(DESs)
critical
for
the
introduction
double
bonds
into
pheromones.
Mythimna
loreyi,
specific
DESs
involved
biosynthesis
remain
unclear.
this
study,
we
identified
and
characterized
25
putative
from
M.
loreyi
genome.
Nineteen
them
were
expressed
female
PGs,
with
seven
showing
significant
upregulation
response
to
activating
neuropeptide
(PBAN).
RNAi-based
knockdown
MlorDES2
significantly
reduced
titer
components
58.6-85.9%,
while
MlorDES9
specifically
production
(Z)-9-tetradecenyl
acetate.
Functional
verification
yeast
revealed
that
exhibited
Δ11
Δ9
desaturase
activities,
respectively.
Taken
together,
these
results
collectively
demonstrate
suggesting
could
be
used
as
potential
targets
pest
management.
Язык: Английский
Is the mating process of Mahanarva spectabilis (Hemiptera: Cercopidae) mediated by chemical signals?
Brazilian Journal of Biology,
Год журнала:
2025,
Номер
85
Опубликована: Янв. 1, 2025
Abstract
Mahanarva
spectabilis
(Distant)
is
an
important
pasture
pest
in
Brazil
and
represents
obstacle
to
forage
production.
Elucidating
the
chemical
signals
involved
communication
of
this
species
essential
for
finding
new
control
alternatives.
Therefore,
aim
study
was
determine
whether
are
mating
process
M.
spectabilis.
In
first
trial,
insect's
behavior
evaluated
cages
with
male:
female
ratios
4:1
1:4.
As
soon
as
copulation
began,
males
or
females
around
copulating
couple
recorded.
subsequent
trials,
olfactory
responses
were
a
Y-shaped
olfactometer
volatiles
released
by
males,
pairs.
At
ratio
4:1,
there
significantly
more
touches
other
couple.
The
number
individuals
attached
time
did
not
differ
relation
used.
attractiveness
pairs
also
observed
olfactometry
test,
which
attracted
only
extract
showed
no
attraction
any
odor
sources
offered.
This
shows
that
Язык: Английский
Evaluation of Reference Genes for Quantitative Real-Time PCR Analysis in the Bean Bug, Riptortus pedestris (Hemiptera: Alydidae)
Insects,
Год журнала:
2023,
Номер
14(12), С. 960 - 960
Опубликована: Дек. 18, 2023
Quantitative
real-time
PCR
(qRT-PCR)
is
widely
accepted
as
a
precise
and
convenient
method
for
quantitatively
analyzing
the
expression
of
functional
genes.
The
data
normalization
strongly
depends
upon
stable
reference
bean
bug,
Riptortus
pedestris
(Hemiptera:
Alydidae),
significant
pest
leguminous
crops
broadly
distributed
across
Southeast
Asia.
In
this
study,
total
16
candidate
genes
(RPL32,
RPS23,
SDHA,
UBQ,
UCCR,
GST,
TATA-box,
HSP70,
GAPDH,
RPL7A,
SOD,
RPS3,
Actin,
α-tubulin,
AK,
EF1)
were
carefully
chosen
in
R.
pedestris,
their
levels
assessed
various
conditions,
including
different
developmental
stages,
diverse
tissues,
temperature
treatments,
adult
age,
molting
time,
mating
status.
Following
this,
stability
these
was
evaluated
using
four
algorithms
(ΔCt,
GeNorm,
NormFinder,
BestKeeper).
Ultimately,
comprehensive
rankings
determined
online
tool
RefFinder.
Our
results
demonstrate
that
gene
qRT-PCR
analysis
contingent
specific
experimental
conditions.
RPL7A
EF1
are
optimal
stages.
Furthermore,
α-tubulin
exhibit
most
tissues.
RPL32
age.
For
nymph
SOD
display
expression.
RPS23
identified
suitable
monitoring
Lastly,
we
verified
practicability
evaluating
odorant-binding
protein
37
(RpedOBP37)
cytochrome
P450
6a2
(RpedCYP6)
throughout
These
findings
addition
to
studies
on
serving
fundamental
groundwork
future
investigations
well
other
organisms.
Язык: Английский
RpedOBP1 plays key roles in aggregation pheromones reception of the Riptortus pedestris
Pesticide Biochemistry and Physiology,
Год журнала:
2024,
Номер
204, С. 106073 - 106073
Опубликована: Авг. 5, 2024
Язык: Английский
Identification and expression profiles of olfactory-related genes in the antennal transcriptome of Graphosoma rubrolineatum (Hemiptera: Pentatomidae)
PLoS ONE,
Год журнала:
2024,
Номер
19(8), С. e0306986 - e0306986
Опубликована: Авг. 6, 2024
Graphosoma
rubrolineatum
(Hemiptera:
Pentatomidae)
is
an
important
pest
of
vegetables
and
herbs
(e.g.,
Umbelliferae
Cruciferae)
in
China,
Siberia,
Korea,
Japan.
Insects
are
highly
dependent
on
their
olfactory
system
to
detect
odorants.
However,
no
molecular-mediated
genes
G
.
have
yet
been
identified.
In
this
study,
we
first
established
the
antennal
transcriptome
identified
189
candidate
genes,
including
31
odorant-binding
proteins
(OBPs),
15
chemosensory
(CSPs),
four
sensory
neuron
membrane
(SNMPs),94
odorant
receptors
(ORs),
23
ionotropic
(IRs),
22
gustatory
(GRs).
Additionally,
phylogenetic
trees
were
constructed
for
between
other
hemipteran
insects.
We
also
detected
expression
profiles
ten
OBPs,
five
CSPs,
two
SNMPs,
ORs,
IRs,
GRs
by
real-time
quantitative
PCR.
The
results
revealed
that
most
(
GrubOBP1/11/31
,
GrubCSP3/8
GrubSNMP1a/1b
GrubOrco/OR9/11/13
GrubGR1/4/22
GrubIR25/75h/76b/GluR1
)
expressed
antennae,
GrubOBP13/31
rubCSP4/11/12
legs,
while
GrubOBP20
GrubGR19
wings.
Our
will
enrich
gene
inventory
provide
further
insight
into
molecular
mechanisms
Язык: Английский
Identification of a Soybean Volatile Attractive for Riptortus pedestris Using Reverse Chemical Ecology Approach
Journal of Agricultural and Food Chemistry,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 27, 2024
The
bean
bug
Riptortus
pedestris
is
a
major
soybean
pest
and
cause
of
the
stay-green
symptoms.
However,
molecular
mechanisms
underlying
its
olfaction-mediated
host-seeking
behavior
remain
unclear.
In
this
study,
we
compared
antennae
transcriptomes
starved
nonstarved
adult
R.
pedestris,
identifying
four
differentially
expressed
odorant
receptor
(OR)
genes.
Among
these,
RpedOR13
showed
strong
response
to
host
volatile
2-phenylethanol
(2-PE)
in
Xenopus
oocyte
assays,
while
electroantennography
behavioral
tests
confirmed
2-PE
as
an
effective
attractant.
Next,
phylogenetic
analysis
identified
RpedOR72b
paralog
RpedOR13,
with
subsequent
assays
confirming
specific
2-PE.
Additionally,
RNA
interference
experiments
highlighted
crucial
role
detecting
Taken
together,
these
findings
provide
new
insights
into
highlight
successful
application
reverse
chemical
ecology
OR-based
screening
bioactive
compounds.
Язык: Английский