A W chromosome-derived feminizing piRNA in pyralid moths demonstrates convergent evolution for primary sex determination signals in Lepidoptera DOI Creative Commons
S.L. Visser, Irena Provazníková, Martina Dalíková

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Июль 26, 2024

Abstract Background The primary signals of sex determination in insects are diverse and evolve erratically. This also appears to be the case with moths butterflies. In silkworm Bombyx mori, female development is triggered by a W-linked Feminizer (Fem) piRNA degrading mRNA Z-linked Masculinizer (Masc) gene, which controls male development. However, Fem absent related Trilocha varians. Since mechanism similar that has evolved phylogenetically distant Plutella xylostella, we investigated whether this conserved other groups Lepidoptera. Results We examined W chromosome Ephestia kuehniella for non-coding feminizing genes identified putative many partial copies EkMasc gene. generated repetitive sequence named E. kuehniella Moth-overruler-of-masculinization (EkMom). EkMom shows high similarity region expressed at onset development, suggesting it likely trigger determination. then mapped small RNA-seq data from embryos Plodia interpunctella PiMasc gene single RNA, PiMom piRNA, able target PiMasc, identity piRNA. Both repeats present copy number form cluster on chromosome. Conclusions Our study provides multiple lines evidence sex-determining signal two pyralid highlights possible pathway origin piRNAs A between proposed P. (Pyralidae) xylostella (Plutellidae) function B. mori (Bombycidae) suggests convergent evolution

Язык: Английский

Sex determination in moths and butterflies: Masculinizer as key player DOI Creative Commons
Arjèn E. van’t Hof, Atsuo Yoshido, František Marec

и другие.

Current Opinion in Insect Science, Год журнала: 2025, Номер unknown, С. 101375 - 101375

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
fmd/fmd2/DHX16‐dsx cascade in sex determination of one Hemiptera species, Laodelphax striatellus DOI
Jun‐Min Li, Zeping Mao,

Yangwei Gao

и другие.

Insect Science, Год журнала: 2025, Номер unknown

Опубликована: Май 6, 2025

Although recent investigations have identified a novel regulatory cascade involving female determiner (fmd), 2 (fmd2), and doublesex (dsx) in Hemiptera species, the evolutionary conservation molecular mechanisms governing fmd/fmd2-dsx pathway remain poorly characterized. The small brown planthopper, Laodelphax striatellus (Hemiptera: Delphacidae), represents an ideal model system for elucidating dynamics of sex determination pathways, as its underlying mechanism remains undefined. Here, we demonstrate that fmd2 homolog (Lsfmd2) L. is functionally involved through specific splicing isoform, LsFMD2315, protein which contains carboxyl-terminal sequence - GTGGGYGGGGKQRGGGRGQRHTPY. Functional characterization 293T cells revealed LsFMD2315 interacts with LsFMD-F to regulate female-specific Lsdsx. Importantly, DHX16, ATP-dependent RNA helicase pre-messenger factor, binding partner LsFMD2315. interference-mediated depletion DHX16 resulted ectopic expression male-specific Lsdsx isoforms individuals induced significant ovipositor malformations. Intriguingly, while exhibited simultaneous capacity both LsDHX16, no direct interaction was observed between LsDHX16 LsFMD-F, indicating serves scaffold fmd/fmd2/DHX16-dsx cascade. These findings provide robust evidence across species. Moreover, newly component significantly enhances our mechanistic understanding this non-canonical pathway.

Язык: Английский

Процитировано

0
Evolution of gene regulatory networks in insects DOI
Takumi Karasawa, Shigeyuki Koshikawa

Current Opinion in Insect Science, Год журнала: 2025, Номер 69, С. 101365 - 101365

Опубликована: Май 8, 2025

Язык: Английский

Процитировано

0
Mosquito sex determination: recent advances and applications DOI

Melanie Hempel,

Joseph D. Warren, Jiangtao Liang

и другие.

Current Opinion in Insect Science, Год журнала: 2025, Номер unknown, С. 101385 - 101385

Опубликована: Май 1, 2025

Язык: Английский

Процитировано

0
Doublesex regulates the development of sex‐specific organs in rice stem borer, Chilo suppressalis DOI
Di Guo, Shuang Zhao,

Shao‐Cong Su

и другие.

Pest Management Science, Год журнала: 2025, Номер unknown

Опубликована: Июнь 2, 2025

The doublesex (dsx) gene is a crucial component of the insect sex determination pathway, playing pivotal role in sexual differentiation and development. While functions dsx have been extensively characterized several species, its molecular mechanisms specific roles remain largely unexplored lepidopteran rice stem borer, Chilo suppressalis (Walker), significant agricultural pest. In this study, we identified two male-specific five female-specific Csdsx transcripts C. suppressalis. Phylogenetic analyses multiple sequence alignments revealed that highly conserved among lepidopterans. To investigate functional roles, employed CRISPR/Cas9-mediated mutagenesis to generate mutants. Specifically, target sites were designed on exons 1, 3, 4, 5 disrupt common region (exon 1), 3 exon 4), regions 5). As anticipated, mutants exhibited abnormal development both external internal genitalia during pupal adult stages. Furthermore, mutations induced sex-specific sterility. Notably, knockout isoforms also resulted wing patterns antennal morphology. This study demonstrates highlights potential as for environmentally friendly pest management strategies against © 2025 Society Chemical Industry.

Язык: Английский

Процитировано

0
Deviations in the Z:A ratio disrupt sexual development in the eri silkmoth, Samia cynthia ricini DOI
Atsuo Yoshido, František Marec

Genetics, Год журнала: 2023, Номер 224(1)

Опубликована: Фев. 16, 2023

Abstract Moths and butterflies (Lepidoptera) have sex chromosome systems with female heterogamety, 2 models, W-dominance Z-counting, been proposed to determine sex. The W-dominant mechanism is well known in Bombyx mori. However, little about the Z-counting Z0/ZZ species. We investigated whether ploidy changes affect sexual development gene expression eri silkmoth, Samia cynthia ricini (2n = 27♀/28♂, Z0♀/ZZ♂). Tetraploid males (4n 56, ZZZZ) females 54, ZZ) were induced by heat cold shock, then, triploid embryos produced crosses between diploids tetraploids. Two karyotypes (3n 42, ZZZ 3n 41, identified embryos. Triploid 3 Z chromosomes showed male-specific splicing of S. doublesex (Scdsx) gene, whereas 2-Z both male- female-specific splicing. From larva adult, 3-Z triploids a normal male phenotype, except for defects spermatogenesis. abnormal gonads observed triploids, which Scdsx transcripts not only but also somatic tissues. Two-Z thus obviously intersexes, suggesting that c. depends on Z:A ratio number. Moreover, mRNA-seq analyses relative levels are similar samples different doses autosome sets. Our results provide first evidence disrupt no effect general mode dosage compensation Lepidoptera.

Язык: Английский

Процитировано

7
Masculinizer gene controls male sex determination in the codling moth, Cydia pomonella DOI
Kristýna Pospíšilová, Arjèn E. van’t Hof, Atsuo Yoshido

и другие.

Insect Biochemistry and Molecular Biology, Год журнала: 2023, Номер 160, С. 103991 - 103991

Опубликована: Авг. 1, 2023

Язык: Английский

Процитировано

6
Doublesex is essential for masculinization but not feminization in Lygus hesperus DOI
J. Joe Hull, Chan C. Heu,

Roni J. Gross

и другие.

Insect Biochemistry and Molecular Biology, Год журнала: 2024, Номер 166, С. 104085 - 104085

Опубликована: Фев. 2, 2024

Язык: Английский

Процитировано

2
Dichotomous sperm in Lepidopteran insects: a biorational target for pest management DOI Creative Commons
Rakesh Kumar Seth, Priya Yadav, Stuart E. Reynolds

и другие.

Frontiers in Insect Science, Год журнала: 2023, Номер 3

Опубликована: Авг. 23, 2023

Lepidoptera are unusual in possessing two distinct kinds of sperm, regular nucleated (eupyrene) sperm and anucleate (apyrene) (‘parasperm’). Sperm both types transferred to the female required for male fertility. Apyrene play ‘helper’ roles, assisting eupyrene gain access unfertilized eggs influencing reproductive behavior mated moths. development promising targets environmentally safer, target-specific biorational control strategies lepidopteran pest insects. dimorphism provides a wide window which manipulate functionality dynamics, thereby impairing fitness species. Opportunities interfere with spermatozoa available not only while still (before copulation), but also (after copulation, when male-provided spermatophore, or during storage female’s spermatheca). Biomolecular technologies like RNAi, miRNAs CRISPR-Cas9 achieve by targeting genes directly indirectly involved dichotomous production, function, persistence.

Язык: Английский

Процитировано

5
Female Sex Determination Factors in Ceratitis capitata: Molecular and Structural Basis of TRA and TRA2 Recognition DOI Creative Commons
Maryanna Martina Perrotta, Francesca Lucibelli, Sarah Maria Mazzucchiello

и другие.

Insects, Год журнала: 2023, Номер 14(7), С. 605 - 605

Опубликована: Июль 4, 2023

In the model system for genetics, Drosophila melanogaster, sexual differentiation and male courtship behavior are controlled by sex-specific splicing of doublesex (dsx) fruitless (fru). vitro in vivo studies showed that female-specific Transformer (TRA) non-sex-specific 2 (TRA2) factors interact, forming a complex promoting dsx fru splicing. TRA/TRA2 binds to 13 nt long sequence repeats their pre-mRNAs. Mediterranean fruitfly Ceratitis capitata (Medfly), major agricultural pest, which shares with ~120 million years old ancestor, Cctra Cctra2 genes seem promote Ccdsx Ccfru, contain conserved binding repeats. Unlike tra, autoregulates its through these putative regulatory Here, yeast two-hybrid assay shows CcTRA interacts CcTRA2, despite high amino acid divergence compared TRA. Interestingly, CcTRA2 itself, as also observed TRA2. We generated three-dimensional formed using predictive approaches based on Artificial Intelligence. This structure identified an evolutionary highly TRA2 recognition motif TRA sequence. The Y2H approach, combined powerful tools protein structures, could use helpful this other insect species understand potential links between different upstream proteins acting primary sex-determining signals transducers.

Язык: Английский

Процитировано

4