Tissue-specific metabolomic signatures for adoublesexmodel of reduced sexual dimorphism DOI Creative Commons
Rene Coig, Benjamin R. Harrison, Richard S. Johnson

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 16, 2024

Abstract Sex has a major effect on the metabolome. However, we do not yet understand degree to which these quantitative sex differences in metabolism are associated with anatomical dimorphism and modulated by sex-specific tissues. In fruit fly, Drosophila melanogaster , knocking out doublesex ( dsx ) gene gives rise adults intermediate characteristics. Here sought determine this key node sexual development leads fly We measured 91 metabolites across head, thorax abdomen comparing between distinctly sex-dimorphic flies those of reduced dimorphism: null flies. Notably, flies, observed difference only 1 metabolites, kynurenate, whereas 51% (46/91) were significantly different wildtype XX XY at least one tissue, suggesting that plays role metabolism. Kynurenate was consistently higher both presence absence functioning . tissue-specific consequences Metabolites affected enriched branched chain amino acid mTOR pathway. This highlights importance considering variation genes cause when analyzing metabolic profiles interpreting their biological significance.

Language: Английский

Sex determination in moths and butterflies: Masculinizer as key player DOI Creative Commons
Arjèn E. van’t Hof, Atsuo Yoshido, František Marec

et al.

Current Opinion in Insect Science, Journal Year: 2025, Volume and Issue: unknown, P. 101375 - 101375

Published: April 1, 2025

Language: Английский

Citations

0
Evolution of gene regulatory networks in insects DOI
Takumi Karasawa, Shigeyuki Koshikawa

Current Opinion in Insect Science, Journal Year: 2025, Volume and Issue: 69, P. 101365 - 101365

Published: May 8, 2025

Language: Английский

Citations

0
Deviations in the Z:A ratio disrupt sexual development in the eri silkmoth, Samia cynthia ricini DOI
Atsuo Yoshido, František Marec

Genetics, Journal Year: 2023, Volume and Issue: 224(1)

Published: Feb. 16, 2023

Abstract Moths and butterflies (Lepidoptera) have sex chromosome systems with female heterogamety, 2 models, W-dominance Z-counting, been proposed to determine sex. The W-dominant mechanism is well known in Bombyx mori. However, little about the Z-counting Z0/ZZ species. We investigated whether ploidy changes affect sexual development gene expression eri silkmoth, Samia cynthia ricini (2n = 27♀/28♂, Z0♀/ZZ♂). Tetraploid males (4n 56, ZZZZ) females 54, ZZ) were induced by heat cold shock, then, triploid embryos produced crosses between diploids tetraploids. Two karyotypes (3n 42, ZZZ 3n 41, identified embryos. Triploid 3 Z chromosomes showed male-specific splicing of S. doublesex (Scdsx) gene, whereas 2-Z both male- female-specific splicing. From larva adult, 3-Z triploids a normal male phenotype, except for defects spermatogenesis. abnormal gonads observed triploids, which Scdsx transcripts not only but also somatic tissues. Two-Z thus obviously intersexes, suggesting that c. depends on Z:A ratio number. Moreover, mRNA-seq analyses relative levels are similar samples different doses autosome sets. Our results provide first evidence disrupt no effect general mode dosage compensation Lepidoptera.

Language: Английский

Citations

7
Doublesex is essential for masculinization but not feminization in Lygus hesperus DOI
J. Joe Hull, Chan C. Heu,

Roni J. Gross

et al.

Insect Biochemistry and Molecular Biology, Journal Year: 2024, Volume and Issue: 166, P. 104085 - 104085

Published: Feb. 2, 2024

Language: Английский

Citations

2
Masculinizer gene controls male sex determination in the codling moth, Cydia pomonella DOI
Kristýna Pospíšilová, Arjèn E. van’t Hof, Atsuo Yoshido

et al.

Insect Biochemistry and Molecular Biology, Journal Year: 2023, Volume and Issue: 160, P. 103991 - 103991

Published: Aug. 1, 2023

Language: Английский

Citations

6
Dichotomous sperm in Lepidopteran insects: a biorational target for pest management DOI Creative Commons
Rakesh Kumar Seth, Priya Yadav, Stuart E. Reynolds

et al.

Frontiers in Insect Science, Journal Year: 2023, Volume and Issue: 3

Published: Aug. 23, 2023

Lepidoptera are unusual in possessing two distinct kinds of sperm, regular nucleated (eupyrene) sperm and anucleate (apyrene) (‘parasperm’). Sperm both types transferred to the female required for male fertility. Apyrene play ‘helper’ roles, assisting eupyrene gain access unfertilized eggs influencing reproductive behavior mated moths. development promising targets environmentally safer, target-specific biorational control strategies lepidopteran pest insects. dimorphism provides a wide window which manipulate functionality dynamics, thereby impairing fitness species. Opportunities interfere with spermatozoa available not only while still (before copulation), but also (after copulation, when male-provided spermatophore, or during storage female’s spermatheca). Biomolecular technologies like RNAi, miRNAs CRISPR-Cas9 achieve by targeting genes directly indirectly involved dichotomous production, function, persistence.

Language: Английский

Citations

4
Single-nucleus transcriptomics of wing sexual dimorphism and scale cell specialization in sulphur butterflies DOI Creative Commons
Ling S. Loh, Joseph J. Hanly, Alexander Carter

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 12, 2024

Abstract The evolution of sexual secondary characteristics necessitates regulatory factors that confer identity to differentiating tissues and cells. In Colias eurytheme butterflies, males exhibit two specialized wing scale types — UV-iridescent (UVI) lanceolate scales absent in females likely integral male courtship behavior. This study investigates the mechanisms single-nucleus transcriptomics underlying these sexually dimorphic cell during development. We show Doublesex (Dsx) expression is itself required repress UVI state females, while unexpectedly, activation independent from Dsx. melanic marginal band, Dsx each sex enforce presence males, their absence females. Single-nucleus RNAseq reveals precursors distinctive gene profiles at 40% pupal development, with marker genes include regulators transcription, signaling, cytoskeletal patterning, chitin secretion. Both male-specific share a low Bric-a-brac ( Bab ) transcription factor, key repressor fate. ChIP-seq profiling suggests binds cis-regulatory regions markers associated fate, including potential effector involved regulation processes secretion, loci showing signatures recent selective sweeps an UVI-polymorphic population. These findings open new avenues for exploring patterning shedding light on driving specification sex-specific states differentiation ultrastructures.

Language: Английский

Citations

1
Female Sex Determination Factors in Ceratitis capitata: Molecular and Structural Basis of TRA and TRA2 Recognition DOI Creative Commons
Maryanna Martina Perrotta, Francesca Lucibelli, Sarah Maria Mazzucchiello

et al.

Insects, Journal Year: 2023, Volume and Issue: 14(7), P. 605 - 605

Published: July 4, 2023

In the model system for genetics, Drosophila melanogaster, sexual differentiation and male courtship behavior are controlled by sex-specific splicing of doublesex (dsx) fruitless (fru). vitro in vivo studies showed that female-specific Transformer (TRA) non-sex-specific 2 (TRA2) factors interact, forming a complex promoting dsx fru splicing. TRA/TRA2 binds to 13 nt long sequence repeats their pre-mRNAs. Mediterranean fruitfly Ceratitis capitata (Medfly), major agricultural pest, which shares with ~120 million years old ancestor, Cctra Cctra2 genes seem promote Ccdsx Ccfru, contain conserved binding repeats. Unlike tra, autoregulates its through these putative regulatory Here, yeast two-hybrid assay shows CcTRA interacts CcTRA2, despite high amino acid divergence compared TRA. Interestingly, CcTRA2 itself, as also observed TRA2. We generated three-dimensional formed using predictive approaches based on Artificial Intelligence. This structure identified an evolutionary highly TRA2 recognition motif TRA sequence. The Y2H approach, combined powerful tools protein structures, could use helpful this other insect species understand potential links between different upstream proteins acting primary sex-determining signals transducers.

Language: Английский

Citations

3
On the Origin and Evolution of the Mosquito Male-determining Factor Nix DOI Creative Commons
James K. Biedler, Azadeh Aryan,

Yumin Qi

et al.

Molecular Biology and Evolution, Journal Year: 2023, Volume and Issue: 41(1)

Published: Dec. 21, 2023

The mosquito family Culicidae is divided into 2 subfamilies named the Culicinae and Anophelinae. Nix, dominant male-determining factor, has only been found in culicines Aedes aegypti albopictus, important arboviral vectors that belong to subgenus Stegomyia. Here we performed sex-specific whole-genome sequencing RNAseq of divergent species explored additional male-inclusive datasets investigate distribution Nix. Except for Culex genus, Nix homologs were all surveyed from subfamily, including 12 3 highly tribes comprising 4 genera, suggesting originated at least 133 165 million years ago (MYA). Heterologous expression 1 open reading frames (ORFs) Ae. resulted partial masculinization genetic females as evidenced by morphology doublesex splicing. Phylogenetic analysis suggests related femaleless (fle), a recently described intermediate sex-determining factor exclusively anopheline mosquitoes. conserved structure, RNA-recognition motifs (RRMs), does fle. However, evolved much faster rate than RRM3 both fle are distantly single RRM widely distributed splicing transformer-2 (tra2). RRM3-based phylogenetic this domain may have tra2 or tra2-related gene common ancestor Our results provide insights evolution sex determination mosquitoes will inform broad applications mosquito-control strategies based on manipulating ratios toward nonbiting males.

Language: Английский

Citations

2
Divergent evolution of male-determining loci on proto-Y chromosomes of the housefly DOI Creative Commons
Xuan Li, S.L. Visser, Jae Hak Son

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: July 16, 2024

Abstract Houseflies provide a good experimental model to study the initial evolutionary stages of primary sex-determining locus because they possess different recently evolved proto-Y chromosomes that contain male-determining loci ( M ) with same gene, Mdmd . We investigate -loci genomically and cytogenetically revealing distinct molecular architectures among -loci. on chromosome V has two intact copies in palindrome. III tandem duplications containing 88 (only one intact) various repeats, including repeats are XY-prevalent. II Y share -like architecture, but fewer repeats. additionally shares -specific sequence arrangements. Based these data karyograms using probes, derives from -specific, we infer histories polymorphic -loci, which have arisen unique translocations , embedded larger DNA fragments, diverged independently into regions varying complexity.

Language: Английский

Citations

0