De Novo Assembly and Characterization of the Transcriptome of an Omnivorous Camel Cricket (Tachycines meditationis) DOI Open Access
Jun-Hui Lu, De‐Long Guan, Sheng‐Quan Xu

и другие.

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(4), С. 4005 - 4005

Опубликована: Фев. 16, 2023

Tachycines meditationis (Orthoptera: Rhaphidophoridae: Tachycines) is a widely distributed insect in eastern Asia. This species common urban environments, and its unique omnivorous diet may contribute to success various habitats. However, molecular studies on the are scarce. Here, we obtained first transcriptome sequence of T. performed preliminary analyses test whether evolution coding sequences fits expectations based species' ecology. We retrieved 476,495 effective transcripts annotated 46,593 (CDS). analysed codon usage found that directional mutation pressure was leading cause bias this species. genome-wide relaxed pattern surprising, given potentially large population size Moreover, despite diet, chemosensory genes do not exhibit deviating significantly from genome-level pattern. They also seem experience more gene family expansion than other cave cricket do. A thorough search for rapidly evolved using dN/dS value showed associated with substance synthesis metabolic pathways, such as retinol metabolism, aminoacyl-tRNA biosynthesis, fatty acid underwent species-specific positive selection. While some results contradict ecology, our assembly provides valuable resource future camel genetics feeding ecology insects, general.

Язык: Английский

Genome editing in pests: basic science to applications DOI
Xien Chen, Subba Reddy Palli

Journal of Pest Science, Год журнала: 2024, Номер 97(3), С. 1135 - 1152

Опубликована: Янв. 24, 2024

Язык: Английский

Процитировано

7
CRISPR/Cas9 mediated editing of pheromone biosynthesis activating neuropeptide (PBAN) gene disrupts mating in the Fall armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) DOI

K. R. Ashok,

Chikmagalur Nagaraja Bhargava,

R. Asokan

и другие.

3 Biotech, Год журнала: 2023, Номер 13(11)

Опубликована: Окт. 15, 2023

Язык: Английский

Процитировано

12
Opsin mutants alter host plant selection by color vision in the nocturnal invasive pest Tuta absoluta DOI Creative Commons

Yanhong Tang,

Siyan Bi,

Xiaodi Wang

и другие.

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 265, С. 130636 - 130636

Опубликована: Март 11, 2024

In insects, vision is crucial in finding host plants, but its role nocturnal insects largely unknown. Vision involves responses to specific spectra of photon wavelengths and opsins plays an important this process. Long-wavelength sensitive opsin (LW opsin) blue-sensitive (BL are main visual proteins play behavior regulation.We used CRISPR/Cas9 technology mutate the long-wavelength-sensitive blue wavelength-sensitive genes explored invasive pest Tuta absoluta. Light wave experiments revealed that LW2 (−/−) BL(−/−) mutants showed abnormal wavelength tropism. Both BL mutations affected preference T. absoluta for green environment. Mutations necessary inhibit attraction. The elimination leaf moths both induced a white plants. Behavioral changes resulting from LW2(−/−) were not by sense smell, further supporting regulatory insect behavior. To best our knowledge, first study reveal vision, host-seeking at night, which key factors. These findings will drive development "vision-ecology" theory.

Язык: Английский

Процитировано

4
Gut bacteria of Spodoptera frugiperda establish endophytic association and affect the interactions of their host herbivore with maize plants DOI
Diandra Achre, Éric Wajnberg, Fernando L. Cônsoli

и другие.

Journal of Pest Science, Год журнала: 2025, Номер unknown

Опубликована: Янв. 28, 2025

Язык: Английский

Процитировано

0
CRISPR/Cas: An emerging molecular technology for biological control of fall armyworm DOI Creative Commons
Ehab El‐Awaad, Hans Merzendorfer

New plant protection., Год журнала: 2025, Номер unknown

Опубликована: Март 5, 2025

Abstract The fall armyworm, Spodoptera frugiperda , is a highly invasive pest that poses severe threats to food crop production worldwide. Several methods have been applied control armyworm infestations, including egg parasitoids, chemical insecticides, and transgenic crops expressing Bacillus thuringiensis (Bt) toxins. However, the currently available are either poorly effective or facing challenges such as resistance development environmental concerns. rapidly evolving molecular tools of genetic engineering, particularly clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated (Cas), increasingly utilized in recent years attempts develop novel management strategies genetically resistant crops. CRISPR/Cas‐based genome editing offers precise efficient tool has used successfully several functional genomic studies over last few years. In this review, we summarize progress using CRISPR/Cas for enabling targeted modification key genes essential survival propagation pest. potential system insect‐resistant approaches can be integrated into current discussed.

Язык: Английский

Процитировано

0
Loss of olfaction reduces caterpillar performance and increases susceptibility to a natural enemy DOI Open Access
Qi Wang, Yufei Jia, Hans M. Smid

и другие.

Опубликована: Март 21, 2025

Insect herbivores such as caterpillars, are under strong selection pressure from natural enemies, especially parasitoid wasps. Although the role of olfaction in host-plant seeking has been investigated great detail parasitoids and adult lepidopteran, caterpillar olfactory system its significance tri-trophic interactions remains poorly understood. In this study, we Pieris brassicae caterpillars importance information among herbivore, Brassica oleracea primary enemy Cotesia glomerata . To examine olfaction, utilized CRISPR/Cas9 to knock out odorant receptor co-receptor ( Orco ). This knockout (KO) impaired detection processing brain. KO exhibited reduced weight lost preference for their optimal food plant. Interestingly, also experienced when challenged by C. whose ovipositor had removed, mortality attack unmanipulated increased. We then behavior P. response volatiles plants attacked conspecific on which were themselves After analyzing volatile compounds involved these interactions, concluded that enables locate suitable sources more efficiently well selecting enemy-free spaces. Our results reveal crucial feeding avoidance, highlighting chemoreceptor genes shaping ecological interactions.

Язык: Английский

Процитировано

0
Loss of olfaction reduces caterpillar performance and increases susceptibility to a natural enemy DOI Open Access
Qi Wang, Yufei Jia, Hans M. Smid

и другие.

Опубликована: Март 21, 2025

Insect herbivores such as caterpillars, are under strong selection pressure from natural enemies, especially parasitoid wasps. Although the role of olfaction in host-plant seeking has been investigated great detail parasitoids and adult lepidopteran, caterpillar olfactory system its significance tri-trophic interactions remains poorly understood. In this study, we Pieris brassicae caterpillars importance information among herbivore, Brassica oleracea primary enemy Cotesia glomerata . To examine olfaction, utilized CRISPR/Cas9 to knock out odorant receptor co-receptor ( Orco ). This knockout (KO) impaired detection processing brain. KO exhibited reduced weight lost preference for their optimal food plant. Interestingly, also experienced when challenged by C. whose ovipositor had removed, mortality attack unmanipulated increased. We then behavior P. response volatiles plants attacked conspecific on which were themselves After analyzing volatile compounds involved these interactions, concluded that enables locate suitable sources more efficiently well selecting enemy-free spaces. Our results reveal crucial feeding avoidance, highlighting chemoreceptor genes shaping ecological interactions.

Язык: Английский

Процитировано

0
Identification of an odorant receptor responding to sex pheromones in Spodoptera frugiperda extends the novel type‐I PR lineage in moths DOI
Sai Zhang, Emmanuelle Jacquin‐Joly, Nicolas Montagné

и другие.

Insect Science, Год журнала: 2023, Номер 31(2), С. 489 - 502

Опубликована: Авг. 12, 2023

Abstract In moths, pheromone receptors (PRs) are crucial for intraspecific sexual communication between males and females. Moth PRs considered as an ideal model studying the evolution of insect PRs, a large number have been identified functionally characterized in different moth species. were initially thought to fall into single monophyletic clade odorant receptor (OR) family, but recent studies shown that ORs another lineage also bind type‐I sex pheromones, which indicates multiple independent origins Lepidoptera. this study, we investigated whether pest Spodoptera frugiperda belonging clades closely related novel PR may capacity pheromones serve male PRs. Among 7 tested, only 1 ( SfruOR23 ) exhibited male‐biased expression pattern. Importantly, vitro functional characterization showed could several compounds with Z ‐9‐tetradecenal 9‐14:Ald), minor component found female glands, optimal ligand. addition, weak responses plant volatile organic compounds. Altogether, S. positioned clade, indicating can be extended moths.

Язык: Английский

Процитировано

10
CRISPR/Cas9: a cutting-edge solution for combatting the fall armyworm, Spodoptera frugiperda DOI

M. N. Rudra Gouda,

H. Jeevan,

H G Shashank

и другие.

Molecular Biology Reports, Год журнала: 2023, Номер 51(1)

Опубликована: Дек. 12, 2023

Язык: Английский

Процитировано

9
Lateralised courtship behaviour and its impact on mating success in Ostrinia furnacalis (Lepidoptera: Crambidae) DOI
Sohail Abbas, Aleena Alam, Muhammad Abbas

и другие.

Bulletin of Entomological Research, Год журнала: 2024, Номер 114(3), С. 374 - 382

Опубликована: Апрель 19, 2024

Abstract Lateralisation is a well-established phenomenon observed in an increasing number of insect species. This study aims to obtain basic details on lateralisation courtship and mating behaviour Ostrinia furnacalis , the Asian corn borer. We conducted laboratory investigations observe behaviours adult O. . Our goal was also detect lateralised variations during sexual interactions elucidate how these variances might influence success males. findings reveal two distinct traits: male approaches from right or left side female direction turning displays. Specifically, males approaching females their predominantly exhibited left-biased 180° displays, while left-side primarily displayed right-biased Notably, males, executing turn for end-to-end genital contact, initiated copulation with fewer attempts began earlier than Furthermore, higher when subsequently approached encounters. Left-biased successful interactions. These observations provide first report reproductive under controlled conditions hold promise establishing reliable benchmarks assessing monitoring quality mass-produced individuals pest control efforts.

Язык: Английский

Процитировано

3