International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(4), С. 4005 - 4005
Опубликована: Фев. 16, 2023
Tachycines
meditationis
(Orthoptera:
Rhaphidophoridae:
Tachycines)
is
a
widely
distributed
insect
in
eastern
Asia.
This
species
common
urban
environments,
and
its
unique
omnivorous
diet
may
contribute
to
success
various
habitats.
However,
molecular
studies
on
the
are
scarce.
Here,
we
obtained
first
transcriptome
sequence
of
T.
performed
preliminary
analyses
test
whether
evolution
coding
sequences
fits
expectations
based
species'
ecology.
We
retrieved
476,495
effective
transcripts
annotated
46,593
(CDS).
analysed
codon
usage
found
that
directional
mutation
pressure
was
leading
cause
bias
this
species.
genome-wide
relaxed
pattern
surprising,
given
potentially
large
population
size
Moreover,
despite
diet,
chemosensory
genes
do
not
exhibit
deviating
significantly
from
genome-level
pattern.
They
also
seem
experience
more
gene
family
expansion
than
other
cave
cricket
do.
A
thorough
search
for
rapidly
evolved
using
dN/dS
value
showed
associated
with
substance
synthesis
metabolic
pathways,
such
as
retinol
metabolism,
aminoacyl-tRNA
biosynthesis,
fatty
acid
underwent
species-specific
positive
selection.
While
some
results
contradict
ecology,
our
assembly
provides
valuable
resource
future
camel
genetics
feeding
ecology
insects,
general.
Abstract
Males
respond
to
intense
sperm
competition
by
adapting
reproductive
strategies
promote
fertilization
success,
which
is
critical
for
population
reproduction.
Thus,
investigating
the
patterns
and
mechanisms
of
crucial
development
application
pest
management
techniques.
In
this
study,
we
analyzed
precedence
pattern
a
major
pest,
fall
armyworm,
used
manage
population.
First,
found
that
females
had
post-mating
response
did
not
gain
direct
benefit
through
multiple
mating.
Next,
in
double
mating
experiment,
molecular
marker
created
CRISPR/Cas9
determine
most
use
only
first
male
produce
offspring.
To
further
explore
role
fertilizing
competition,
constructed
sterile
line
with
eupyrene
defect
knocking
out
B2t
gene.
Interestingly,
two
round
assays
showed
-null
males
inhibited
from
second
wild-type
male.
other
words,
prior
significantly
reduced
fertility
fecundity
females.
Based
on
finding,
continued
whether
sperm-deficient
could
be
FAW
populations.
Cage
experiments
mathematical
modeling
analyses
release
excess
induced
suppression.
Our
study
expands
our
knowledge
lepidopteran.
addition,
provides
paradigm
develop
apply
genetic
control
methods
based
outcome
polyandrous
pests.
Significance
Sperm
essential
maintaining
Understanding
facilitates
appropriate
methods.
Here,
describe
globally
armyworm
displays
first-male
pattern.
males,
produces
non-functional
sperm,
reduces
That
means
ejaculate
male,
even
if
its
are
defective,
can
inhibit
this,
suppresses
These
results
suggest
future
techniques
targeting
nucleated
effectively
Frontiers in Physiology,
Год журнала:
2023,
Номер
14
Опубликована: Июль 14, 2023
A
sophisticated
and
sensitive
olfactory
system
plays
a
vital
role
in
the
survival
reproduction
of
insects.
Chemosensory
receptors
are
indispensable
for
molecular
recognition
discrimination
semiochemicals.
Riptortus
pedestris
is
notorious
pest
legume
plants,
resulting
yield
losses
quality
decreases
soybeans.
It
well
accepted
that
R.
highly
relies
on
its
detecting
aggregation
pheromones,
host
volatiles,
pesticides;
however,
little
research
focused
chemosensory
receptors.
In
present
study,
we
identified
237
odorant
(ORs),
42
gustatory
(GRs),
31
ionotropic
(IRs)
from
reported
genome
pedestris,
analyzed
their
phylogenetic
relationship
with
other
hemipteran
species.
Through
results
RNA-seq
real-time
quantitative
PCR
(qRT-PCR),
found
RpedORs
displayed
different
expression
levels
antennae
at
development
stages.
To
further
verify
function
receptor
co-receptor
(Orco),
an
obligate
unique
insect
OR,
silenced
RpedOrco
by
RNA
interference
(RNAi)
method.
The
showed
silencing
could
significantly
impair
response
to
pheromone
indicating
essential
detection.
Our
can
provide
theoretical
foundations
revealing
mechanism
help
explore
develop
novel
olfactory-based
agents
against
this
pest.
Journal of Agricultural and Food Chemistry,
Год журнала:
2024,
Номер
72(33), С. 18353 - 18364
Опубликована: Авг. 6, 2024
(Lepidoptera:
Erebidae)
is
difficult
and
costly
to
control
as
a
quarantine
pest
found
globally.
Sex
pheromone
trapping
an
effective
measure
for
its
population
monitoring
control;
however,
the
peripheral
neural
mechanism
of
sex
recognition
in
Biomolecules,
Год журнала:
2024,
Номер
14(9), С. 1074 - 1074
Опубликована: Авг. 27, 2024
The
fall
armyworm
(Spodoptera
frugiperda)
poses
a
substantial
threat
to
many
important
crops
worldwide,
emphasizing
the
need
develop
and
implement
advanced
technologies
for
effective
pest
control.
CRISPR/Cas9,
derived
from
bacterial
adaptive
immune
system,
is
prominent
tool
used
genome
editing
in
living
organisms.
Due
its
high
specificity
adaptability,
CRISPR/Cas9
system
has
been
various
functional
gene
studies
through
knockout
applied
research
engineer
phenotypes
that
may
cause
economical
losses.
practical
application
of
diverse
insect
orders
also
provided
opportunities
developing
strategies
genetic
control,
such
as
drive
precision-guided
sterile
technique
(pgSIT).
In
this
review,
comprehensive
overview
recent
progress
S.
frugiperda
presented.
We
outline
fundamental
principles
applying
embryonic
microinjection
highlight
study
genes
associated
with
biological
aspects,
including
body
color,
insecticide
resistance,
olfactory
behavior,
sex
determination,
development,
RNAi.
ability
technology
induce
sterility,
disrupt
developmental
stages,
influence
mating
behaviors
illustrates
roles
management
strategies.
Furthermore,
review
addresses
limitations
studying
function
explores
future
potential
promising
controlling
pest.
Insects,
Год журнала:
2024,
Номер
15(9), С. 660 - 660
Опубликована: Авг. 30, 2024
Olfaction
and
gustation
processes
play
key
roles
in
the
life
cycle
of
insects,
such
as
finding
accepting
food
sources,
oviposition
sites,
mates,
among
other
fundamental
aspects
insect
development.
In
this
context,
chemosensory
genes
found
sensory
organs
(e.g.,
antennae
maxillary
palps)
are
crucial
for
understanding
behaviour,
particularly
phytophagous
behaviour
pests
that
attack
economically
important
crops.
An
example
is
scarab
beetle
