The Evolution of Antimicrobial Resistance in Acinetobacter baumannii and New Strategies to Fight It
Antibiotics,
Год журнала:
2025,
Номер
14(1), С. 85 - 85
Опубликована: Янв. 14, 2025
Acinetobacter
baumannii
is
considered
one
of
the
prioritized
ESKAPE
microorganisms
for
research
and
development
novel
treatments
by
World
Health
Organization,
especially
because
its
remarkable
persistence
drug
resistance.
In
this
review,
we
describe
how
can
be
acquired
enzymatic
degradation
antibiotics,
target
site
modification,
altered
membrane
permeability,
multidrug
efflux
pumps,
their
ability
to
form
biofilms.
Also,
evolution
resistance
in
A.
baumannii,
which
mainly
driven
mobile
genetic
elements,
reported,
with
particular
reference
plasmid-associated
resistance,
islands,
insertion
sequences.
Finally,
an
overview
existing,
new,
alternative
therapies
provided.
Язык: Английский
Prophylactic phage administration provides a time window for delayed treatment of vancomycin-resistant Enterococcus faecalis in a murine bacteremia model
Frontiers in Microbiology,
Год журнала:
2025,
Номер
15
Опубликована: Янв. 24, 2025
Introduction
Vancomycin-resistant
Enterococcus
faecalis
(VRE)
poses
a
significant
challenge
in
clinical
settings
due
to
its
resistance
multiple
antibiotics.
Phage
therapy
offers
promising
alternative
address
this
crisis.
However,
critical
gaps
remain
regarding
optimal
dosing,
therapeutic
design,
and
treatment
timing
for
phage
targeting
VRE-induced
bacteremia.
Methods
The
biological
genomic
characteristics
of
novel
lytic
specific
VRE
were
investigated.
Its
vitro
bactericidal
antibiofilm
activities
evaluated,
along
with
synergy
antimicrobial
agents.
In
safety
protective
efficacy
assessed
using
mouse
bacteremia
model.
impact
on
gut
microbiota
was
examined
through
16S
rDNA
gene
sequencing.
Results
We
isolated
characterized
phage,
vB_EfaS-1017,
vancomycin-resistant
E.
.
This
features
circular,
double-stranded
DNA
genome
(40,766
bp),
sharing
91.19%
identity
79%
coverage
vB_EfaS_SRH2.
vB_EfaS-1017
exhibited
robust
activity
demonstrated
levofloxacin.
Safety
assessments
confirmed
non-toxicity
mammalian
cells
lack
hemolytic
activity.
model,
alone
rescued
60%
infected
mice,
while
combining
levofloxacin
increased
survival
80%.
Prophylactic
administration
24
hours
prior
infection
failed
prevent
mortality.
combination
prophylactic
delayed
compared
100%
mortality
the
group.
Additionally,
helped
maintain
or
restore
balance.
Discussion
These
findings
underscore
potential
phage-antibiotic
combinations
as
superior
strategy
against
infections.
observed
between
phages
antibiotics
highlights
approach
overcoming
bacterial
improving
outcomes.
Furthermore,
may
provide
time
window
effective
treatment.
Further
preclinical
research
is
essential
refine
protocols
application.
Язык: Английский
Phage therapy: an alternative treatment modality for MDR bacterial infections
Infectious Diseases,
Год журнала:
2024,
Номер
56(10), С. 785 - 817
Опубликована: Июль 17, 2024
The
increasing
global
incidence
of
multidrug-resistant
(MDR)
bacterial
infections
threatens
public
health
and
compromises
various
aspects
modern
medicine.
Recognising
the
urgency
this
issue,
World
Health
Organisation
has
prioritised
development
novel
antimicrobials
to
combat
ESKAPEE
pathogens.
Comprising
Язык: Английский
Phage vB_Kpn_HF0522: Isolation, Characterization, and Therapeutic Potential in Combatting K1 Klebsiella pneumoniae Infections
Infection and Drug Resistance,
Год журнала:
2025,
Номер
Volume 18, С. 803 - 818
Опубликована: Фев. 1, 2025
Purpose:
Klebsiella
pneumoniae
is
a
globally
prevalent
pathogen
responsible
for
severe
hospital-
and
community-acquired
infections,
presents
significant
challenges
clinical
management.
Current
therapeutic
strategies
are
no
longer
able
to
meet
the
needs;
therefore,
there
an
urgent
need
develop
novel
strategies.
This
study
aimed
evaluate
efficacy
of
phage
therapy
in
treating
bacterial
infections.
Methods:
Isolated
vB_Kpn_HF0522
morphology
were
observed
using
transmission
electron
microscopy.
Analysis
characteristics,
including
optimal
multiplicity
infection
(MOI),
one-step
growth
curve,
host
range,
stability
different
environments,
adsorption
capacity.
The
genomic
sequence
was
analyzed
explore
evolutionary
relationships.
effect
on
biofilms
assessed
crystal
violet
staining
assay.
Galleria
mellonella
(
G.
)
model
mouse
models
established
practical
application
potential
fitness
cost
phage-resistant
bacteria.
Results:
Phage
isolated
from
hospital
sewage
experimental
studies.
Genome
analysis
revealed
that
double-stranded
linear
DNA
virus.
Biological
characterization
demonstrated
this
specifically
targets
serotype
K1
K.
with
(MOI)
0.01,
effectively
disrupting
inhibiting
growth.
rate
remained
largely
unchanged
after
resistance
mutation,
but
mice
infected
mutant
strain
showed
significantly
higher
survival
rates
than
those
wild-type
strain.
increased
12.5%
75%,
inhibited
incisional
surgical
site
infections
alleviated
inflammatory
response
mice.
Conclusion:
These
findings
indicate
has
specific
may
serve
as
antimicrobial
agent
research
anti-infective
therapy.
Keywords:
vB_Kpn_HF0522,
therapy,
,
resistance,
infection,
biofilm
Язык: Английский
Optimizing phage therapy for carbapenem-resistant Enterobacter cloacae bacteremia: insights into dose and timing
Antimicrobial Agents and Chemotherapy,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 26, 2025
ABSTRACT
The
increase
in
multidrug-resistant
(MDR)
Enterobacter
cloacae
complex
(ECC)
infections,
particularly
those
resistant
to
carbapenems,
underscores
the
urgent
need
for
alternative
therapies.
Phage
therapy,
with
its
specific
bactericidal
action,
offers
a
promising
solution.
However,
there
remains
shortage
of
well-characterized
ECC-targeting
phages,
and
dosing
timing
optimization
ECC-specific
phage
cocktails
is
largely
unexplored.
In
this
study,
we
isolated
characterized
three
novel
lytic
phages
diverse
genome
sizes
host
ranges.
Notably,
ФEBU8
demonstrated
broad-spectrum
activity,
lysing
both
species
Acinetobacter
baumannii
.
ФECL22
displayed
stability
across
wide
temperature
range
(4–50°C),
pH
tolerance
(6–10),
burst
size
19
PFU/cell,
OmpA
identified
as
receptor.
Our
formulated
cocktail,
comprising
ФEBU8,
ФECL22,
ФECL30,
effectively
rescued
mice
E.
bacteremia
dose-dependent
manner,
mid-dose
regimen
showing
strong
efficacy.
Immediate
administration
achieved
full
survival,
whereas
combined
prophylactic
therapeutic
(“−24
+
6”)
also
resulted
100%
survival.
These
findings
highlight
critical
roles
optimizing
therapy
carbapenem-resistant
use
providing
valuable
window
delayed
treatment
strategy
combating
severe
bacterial
infections.
Язык: Английский
Characterization of a novel lytic phage vB_AbaM_AB4P2 encoding depolymerase and its application in eliminating biofilms formed by Acinetobacter baumannii
BMC Microbiology,
Год журнала:
2025,
Номер
25(1)
Опубликована: Март 8, 2025
Acinetobacter
baumannii
strains
are
a
primary
cause
of
hospital-acquired
infections.
This
bacterium
frequently
causes
biofilm-related
infections,
notably
ventilator-associated
pneumonia
and
catheter-related
which
exhibit
remarkable
resistance
to
antibiotic
treatment,
posing
severe
challenge
in
the
prevention
A.
Therefore,
strategies
eliminate
biofilm
catheters
becoming
increasingly
important.
Phages
capable
lysing
bacteria
have
certain
effect
on
ablation
biofilms.
Sewage
treatment
plant
water
was
collected
for
isolation
phages.
The
morphological,
host
range,
one-step
growth,
temperature
pH
stability,
bactericidal
activity,
sequencing
genomic
analysis
were
performed
characterize
isolated
phage.
three-dimensional
structure
tail
fiber
protein
predicted
by
AlphaFold3.
efficacy
phage
clearing
biofilms
from
24-well
plates
PVC
also
evaluated.
In
this
study,
lytic
vB_AbaM_AB4P2
sewage
water,
showing
clear
plaque
with
halo
zone.
One-step
growth
assays
unveiled
20-minute
latent
period
burst
size
61
forming
unit/cell
(PFU/cell).
At
same
time,
AB4P2
exhibited
stability
at
3–11
temperatures
30–70
°C.
Its
dsDNA
genome
is
composed
45,680
bp
G
+
C
content
46.13%.
Genomic
phylogenetic
situated
as
new
species
Caudoviricetes
class.
possesses
pectin
lyase-like
domain
that
linked
depolymerase
playing
crucial
role
disrupting
Additionally,
it
encodes
lysis
cassette
comprising
endolysin,
holin
Rz-like
spanin,
yet
lacks
any
genes
responsible
virulence
factors.
Phage
can
completely
inhibit
16
h.
plate
polyvinyl
chloride
(PVC)
catheter
model
experiments,
achieved
significant
rate
effectively
killed
live
bacterial
cells
biofilm.
had
good
environmental
strong
ability
destroy
formed
baumannii.
It
exhibits
promising
potential
development
an
alternative
disinfectant
against
hospital.
Not
applicable.
Язык: Английский
Recent insights on phage therapy against multidrug-resistant Acinetobacter baumannii
AMB Express,
Год журнала:
2025,
Номер
15(1)
Опубликована: Март 12, 2025
Abstract
Acinetobacter
baumannii
is
a
prevalent
clinical
pathogen
commonly
found
to
be
multidrug-resistant
(MDR),
causing
serious
life-threatening
infections,
particularly
hospital-acquired
infections
with
limited
therapeutic
options.
The
MDR
phenotype
developed
against
this
critical
increasingly
globally,
reaching
pan-drug-resistant
conferring
non-susceptibility
all
antimicrobials
used
in
its
treatment
according
the
standard
guidelines.
Therefore,
it
develop
innovative
approaches,
such
as
phage
therapy,
considering
rise
drug-resistant
A.
infections.
In
review,
we
highlight
and
discuss
up-to-date
antimicrobial
resistance
of
,
use
phages,
their
limitations,
future
perspectives
treating
addition,
combination
phages
antimicrobials,
preclinical
studies
including
pharmacokinetics
pharmacodynamics
properties
have
been
discussed.
Язык: Английский
Phage Therapy: An Alternative Approach to Combat Multi-drug Resistant Infections in Cystic Fibrosis
Опубликована: Июнь 21, 2024
In
this
review
we
aim
to
address
the
current
status
of
phage
therapy
in
management
multidrug-resistant
infections,
from
compassionate
use
cases
ongoing
clinical
trials,
as
well
challenges
approach
presents
particular
context
CF
patients.
Язык: Английский
Phage Therapy: An Alternative Approach to Combating Multidrug-Resistant Bacterial Infections in Cystic Fibrosis
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(15), С. 8321 - 8321
Опубликована: Июль 30, 2024
Patients
with
cystic
fibrosis
(CF)
are
prone
to
developing
life-threatening
lung
infections
a
variety
of
pathogens
that
difficult
eradicate,
such
as
Burkholderia
cepacia
complex
(Bcc),
Hemophilus
influenzae,
Mycobacterium
abscessus
(Mab),
Pseudomonas
aeruginosa,
and
Staphylococcus
aureus.
These
still
remain
an
important
issue,
despite
the
therapy
for
CF
having
considerably
improved
in
recent
years.
Moreover,
prolonged
exposure
antibiotics
combination
favors
development
spread
multi-resistant
bacteria;
thus,
alternative
strategies
is
crucial
counter
antimicrobial
resistance.
In
this
context,
phage
therapy,
i.e.,
use
phages,
viruses
specifically
infect
bacteria,
has
become
promising
strategy.
review,
we
aim
address
current
status
management
multidrug-resistant
infections,
from
compassionate
cases
ongoing
clinical
trials,
well
challenges
approach
presents
particular
context
patients.
Язык: Английский
Phage-Based Therapy in Combination with Antibiotics: A Promising Alternative against Multidrug-Resistant Gram-Negative Pathogens
Pathogens,
Год журнала:
2024,
Номер
13(10), С. 896 - 896
Опубликована: Окт. 14, 2024
The
continued
rise
in
antimicrobial
resistance
poses
a
serious
threat
to
public
health
worldwide.
use
of
phages
that
can
have
bactericidal
activity
without
disrupting
the
normal
flora
represents
promising
alternative
treatment
method.
This
practice
has
been
successfully
applied
for
decades,
mainly
Eastern
Europe,
and
recently
used
as
an
emergency
therapy
compassionate
care
United
States.
Here,
we
provide
comprehensive
review
pre-clinical
clinical
applications
phage
concerning
three
major
Gram-negative
pathogens:
Язык: Английский