Eucommia ulmoides polysaccharide modified nano-selenium effectively alleviated DSS-induced colitis through enhancing intestinal mucosal barrier function and antioxidant capacity

Journal of Nanobiotechnology, Год журнала: 2023, Номер 21(1)

Опубликована: Июль 12, 2023

Abstract Ulcerative colitis (UC) is currently the most common inflammatory bowel disease (IBD). Due to its diverse and complex causes, there no cure at present, researchers are constantly exploring new therapies. In recent years, nano-selenium particle(SeNP) has attracted wide attention due excellent biological activities. Therefore, in this study, for first time, we used a natural polysaccharide, Eucommia ulmoides polysaccharide (EUP), modified SeNP get EUP-SeNP with size of about 170 nm, effect on 3% dextran sulphate sodium (DSS) induced was explored. Our results showed that colon intestinal histology, mucosal barrier, cytokines microbiome composition were changed after treatment mice. Specifically, it also shown oral could relieve degree DSS-induced mice by restoring weight loss, reducing activity index (DAI), enhancing antioxidant capacity regulating composition. addition, verified mechanism epithelial cell lines, showing inhibited LPS-induced activation TRL-4/NF-κB signaling pathway lines. To some extend, our study provides therapeutic reference IBD.

Язык: Английский

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Chitosan and Chitooligosaccharide: The Promising Non-Plant-Derived Prebiotics with Multiple Biological Activities

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(12), С. 6761 - 6761

Опубликована: Июнь 17, 2022

Biodegradable chitin is the second-most abundant natural polysaccharide, widely existing in exoskeletons of crabs, shrimps, insects, and cell walls fungi. Chitosan chitooligosaccharide (COS, also named chitosan oligosaccharide) are two most important deacetylated derivatives chitin. Compared with chitin, COS not only have more satisfactory physicochemical properties but exhibit additional biological activities, which cause them to be applied fields food, medicine, agriculture. Additionally, due their significant ability improve gut microbiota, deemed prospective prebiotics. Here, we introduced production, properties, applications, pharmacokinetic characteristics COS. Furthermore, summarized latest research on antioxidant, anti-inflammatory, antimicrobial activities. Research progress prebiotic functions particularly reviewed. We creatively analyzed discussed mechanisms correlations underlying these activities properties. Our work enriched people's understanding non-plant-derived Based this review, future directions explored. Collectively, optimizing production technology enriching will shed light capability human health.

Язык: Английский

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Dynamic Changes in Gut Microbiome of Ulcerative Colitis: Initial Study from Animal Model

Journal of Inflammation Research, Год журнала: 2022, Номер Volume 15, С. 2631 - 2647

Опубликована: Апрель 1, 2022

Background: An animal model of DSS-induced UC has been widely used in basic research, and the dysbiosis gut microbiome is one important pathogenetic mechanisms UC, but its dynamic changes correlation with inflammatory factors are not clear yet. Methods: Clinical signs tissue damage degree C57BL/6 ulcerative colitis mice induced by different concentrations DSS were compared that normal mice, finally optimal concentration was determined. Then we analyzed sequencing results to determine patterns their factors. Results: at 2.5% 3.0% could cause intestinal injury induce colitis. However, resulted higher mortality. In addition, there model: relative abundance flora increased first then decreased Bacteroides , Parabacteroides Romboutsia Clostridium_sensu_stricto_1, Lachnospiraceae_NK4A136_group, norank_f_norank_o_Clostridia_UCG-014, Parasutterella Lactobacillus Muribaculum norank_f_Muribaculaceae, Bifidobacterium Coriobacteriaceae_UCG-002 Enterorhabdus did change 14 days significantly on day 21. Moreover, cytokines closely associated imbalance microbiota UC: most pathogenic bacteria tract positively correlated pro-inflammatory negatively anti-inflammatory factors, while beneficial opposite. Conclusion: Intestinal microecology plays an role model, dynamically occurrence development Keywords: colitis, DSS, microbiome, changes, 16S rDNA

Язык: Английский

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Lactobacillus rhamnosus and L. plantarum Combination Treatment Ameliorated Colitis Symptoms in a Mouse Model by Altering Intestinal Microbial Composition and Suppressing Inflammatory Response

Molecular Nutrition & Food Research, Год журнала: 2023, Номер 67(11)

Опубликована: Март 29, 2023

Scope Changes in composition of intestinal microbes may disrupt the balance their interaction with a susceptible host, resulting development inflammatory bowel disease (IBD). Methods and results The study applied combination two Lactobacillus strains ( L. rhamnosus BY‐02, plantarum BY‐05) (“LS treatment”), previously isolates from feces healthy human infants, mouse model dextran sodium sulfate (DSS)‐induced colitis, evaluates ameliorative effect its possible mechanism. LS treatment suppresses weight loss colon shortening, reduces activity index mice. It also has several additional beneficial effects: i) maintains goblet cell numbers ameliorates barrier damage colonic tissue; ii) alters microbial close to normal by increasing abundances Muribaculaceae , Akkermansia Clostridia Oscillospiraceae Lachnospiraceae decreasing abundance Escherichia‐Shigella ; iii) increases content short‐chain fatty acids; iv) pro‐inflammatory lipopolysaccharides; v) overactivation TLR4/NF‐κB signaling pathway. Conclusion Combination strongly colitis symptoms favorably altering suppressing response.

Язык: Английский

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The role of functional oligosaccharides as prebiotics in ulcerative colitis

Food & Function, Год журнала: 2022, Номер 13(13), С. 6875 - 6893

Опубликована: Янв. 1, 2022

An overview of the definitions, functions, mechanisms and applications functional oligosaccharides in colitis prevention treatment.

Язык: Английский

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Effect of Probiotic Fungi against Cognitive Impairment in Mice via Regulation of the Fungal Microbiota–Gut–Brain Axis

Journal of Agricultural and Food Chemistry, Год журнала: 2022, Номер 70(29), С. 9026 - 9038

Опубликована: Июль 14, 2022

The fungal microbiota may be involved in the regulation of cognition and behavior, while role probiotic fungi against cognitive impairment is unclear. Here, we explored idea that Saccharomyces boulardii could participate microglia-induced neuroinflammation Alzheimer's disease (AD) model mice. Cognitive deficits, deposits amyloid-β (Aβ) phosphorylation tau, synaptic plasticity, microglia activation, neuroinflammatory reactions were observed. expression levels Toll-like receptors (TLRs) pathway-related proteins detected. Meanwhile, intestinal barrier integrity composition evaluated. Our results showed dysbiosis APP/PS1 mice, which might result AD. increased interleukin (IL)-6, IL-1β, cluster differentiation 11b (CD11b) observed associated with activation microglia, indicative a broader recognition mediated by compared to hitherto appreciated. Probiotic S. treatment improved dysbiosis, alleviated as well injury, ultimately impairment. Moreover, therapy inhibit TLRs pathway, reversed antifungal treatment. These findings revealed actively participated regulating pathway via gut-brain axis.

Язык: Английский

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The epithelial barrier: The gateway to allergic, autoimmune, and metabolic diseases and chronic neuropsychiatric conditions

Seminars in Immunology, Год журнала: 2023, Номер 70, С. 101846 - 101846

Опубликована: Окт. 4, 2023

Since the 1960 s, our health has been compromised by exposure to over 350,000 newly introduced toxic substances, contributing current pandemic in allergic, autoimmune and metabolic diseases. The "Epithelial Barrier Theory" postulates that these diseases are exacerbated persistent periepithelial inflammation (epithelitis) triggered a wide range of epithelial barrier-damaging substances as well genetic susceptibility. barrier serves body's primary physical, chemical, immunological against external stimuli. A leaky facilitates translocation microbiome from surface afflicted tissues interepithelial even deeper subepithelial locations. In turn, opportunistic bacterial colonization, microbiota dysbiosis, local impaired tissue regeneration remodelling follow. Migration inflammatory cells susceptible contributes damage inflammation, initiating aggravating many chronic objective this review is highlight evaluate recent studies on physiology its role pathogenesis light theory.

Язык: Английский

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Clostridium butyricum and Chitooligosaccharides in Synbiotic Combination Ameliorate Symptoms in a DSS-Induced Ulcerative Colitis Mouse Model by Modulating Gut Microbiota and Enhancing Intestinal Barrier Function

Microbiology Spectrum, Год журнала: 2023, Номер 11(2)

Опубликована: Март 28, 2023

Effects of Clostridium butyricum and chitooligosaccharides (COS), singly in synbiotic combination, were evaluated a C57BL/6 mouse model dextran sulfate (DSS)-induced acute ulcerative colitis (UC). Treatment with C. and/or COS ameliorated UC symptoms vivo, the strongest effects observed for combination terms reduced mortality rates disease activity indices, increased body weight colon length, improved histological features. The achieved following: (i) regulated levels inflammation-related cytokines (tumor necrosis factor alpha [TNF-α], interleukin-1β [IL-1β], IL-6, IL-10) had stronger anti-inflammatory effect than either component alone, based on inhibition Toll-like receptor 4 (TLR-4)/NF-κB/MAPK signaling pathway activation; (ii) enhanced intestinal barrier function by restoring tight junction proteins (occludin, claudin-1, ZO-1) MUC2; (iii) abundance diversity beneficial bacteria (gut microbiota) pathogenic bacteria; (iv) production short-chain fatty acids. Our findings indicate that has strong potential as therapeutic adjuvant UC. IMPORTANCE Ulcerative (UC), an idiopathic characterized continuous remission/relapse inflammatory cycles colonic mucosal layer, adverse patients' quality life considerable costs health care systems. Probiotics, prebiotics, synbiotics are regarded agents UC, safety efficacy. In this study, we present detailed evaluation DSS-induced composed (molecular [MW], 2,500 Da). We found synergistic (synbiotic) action is more effective alone prevention therapy regulating gut microbiota function. development anti-UC drugs or pharmaceutical, food, livestock industries. Highlights include following. clinical morphology. displayed antioxidant effects. expression proteins. inhibited TRL-4/NF-κB/MAPK pathway. (v) modulated composition.

Язык: Английский

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Ameliorative effect of an acidic polysaccharide from Phellinus linteus on ulcerative colitis in a DSS-induced mouse model

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 265, С. 130959 - 130959

Опубликована: Март 17, 2024

Язык: Английский

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Effects of chitosan oligosaccharides (COS) and FMT from COS-dosed mice on intestinal barrier function and cell apoptosis

Carbohydrate Polymers, Год журнала: 2022, Номер 297, С. 120043 - 120043

Опубликована: Авг. 29, 2022

Язык: Английский

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