Unraveling the Impact of Gut and Oral Microbiome on Gut Health in Inflammatory Bowel Diseases

Nutrients, Год журнала: 2023, Номер 15(15), С. 3377 - 3377

Опубликована: Июль 29, 2023

Inflammatory bowel disease (IBD) is a complex disorder characterized by chronic inflammation of the gastrointestinal tract (GIT). IBD mainly includes two distinct diseases, namely Crohn's and ulcerative colitis. To date, precise etiology these conditions not fully elucidated. Recent research has shed light on significant role oral gut microbiome in development progression its collective influence health. This review aims to investigate connection between context IBD, exploring intricate interplay microbial communities their impact overall advances have revealed compelling link microbiome, highlighting pivotal maintaining The cavity GIT are interconnected ecosystems that harbor implicated pathogenesis several ways. Reduction diversity abundance beneficial bacterial species with colonization opportunistic pathogens can induce inflammation. Some arise from origin, especially patients diseases such as periodontitis. It essential discern mechanisms transmission, health potential dysbiosis development. By elucidating this relationship, we enhance our understanding identify therapeutic avenues for managing disease. Furthermore, innovative strategies modulating promote prevent occurrence progression.

Язык: Английский

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Hydrogel Derived from Decellularized Porcine Small Intestinal Submucosa as a Physical Shielding Repaired the Gut Epithelial Barrier of Murine Ulcerative Colitis

Advanced Functional Materials, Год журнала: 2024, Номер 34(45)

Опубликована: Июнь 3, 2024

Abstract Ulcerative colitis (UC) is associated with the shedding of gut mucus. Decellularized porcine small intestinal submucosa (SIS‐ECM) contains numerous active forms extracellular matrix as well growth factors. Herein, a mucus‐mimetic hydrogel (MAG) designed barrier therapy by integrating SIS‐ECM hyaluronic acid and epigallocatechin‐3‐gallate. MAG not only presented viscoelastic behaviors G' 4000 Pa but also good thixotropy. Moreover, physical shielding effective to hinder E. coli invasion. Besides, repairing effect on injured Caco‐2 cells MAG. When biomimetic mucus rectally administrated mouse, it alleviated effectively symptoms improved colonic shorting. expression IL‐1β, IL‐6, TNF‐α at laminae propria substantially inhibited epitheliums are rearranged, tight junction proteins (ZO‐1, Occludin‐1, Claudin‐5) between them greatly upregulated after treatment. Meanwhile, secretion goblets repaired The mechanism mucosa barriers promoting proliferation differentiation stem cells. Collectively, might be promising strategy for UC.

Язык: Английский

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Berberine Depresses Inflammation and Adjusts Smooth Muscle to Ameliorate Ulcerative Colitis of Cats by Regulating Gut Microbiota

Microbiology Spectrum, Год журнала: 2022, Номер 10(6)

Опубликована: Окт. 26, 2022

Intestinal microbiota dysbiosis is a well established characteristic of ulcerative colitis (UC). Regulating the gut an effective UC treatment strategy. Berberine (BBR), alkaloid extracted from several Chinese herbs, common traditional medicine. To establish efficacy and mechanism action BBR, we constructed model using healthy adult shorthair cats to conduct systematic study colonic tissue pathology, inflammatory factor expression, structure. We investigated therapeutic capacity BBR for regulating thus work against in 16S rRNA genes amplicon sequencing technology. Our results revealed that dextran sulfate sodium (DSS)-induced cat models showed weight loss, diarrhea accompanied by mucous blood, histological abnormalities, shortening colon, all which were significantly alleviated supplementation with BBR. A gene-based analysis demonstrated could benefit microbiota. Western blot, quantitative PCR, enzyme-linked immunosorbent assays (ELISAs) DSS-induced models, expression factors was increased, activating JAK2/STAT3 signaling pathway, reversed this effect. The myosin light chain (MLC) phosphorylation smooth muscle intestines associated motility inflammation-related cats. This used flora analyses demonstrate anti-UC effects its potential mechanisms offers novel insights into prevention diseases natural products. IMPORTANCE Ulcerative (UC) clinics. disorder correlated colitis. Although there are many studies on rats, few Therefore, explored possibility use as safe efficient based state intestinal flora. found be colitis, damage, dysbiosis.

Язык: Английский

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Crucial role of low molecular weight chondroitin sulfate from hybrid sturgeon cartilage in osteoarthritis improvement: Focusing on apoptosis, systemic inflammation, and intestinal flora

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 139850 - 139850

Опубликована: Янв. 1, 2025

Язык: Английский

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Synergistic protective effects of probiotics Lactiplantibacillus plantarum S58 and prebiotics Qingke β-glucan on dextran sulfate sodium-induced cognitive deficits in colitis mice: A study of microbial-gut-brain axis modulation

Food Bioscience, Год журнала: 2025, Номер unknown, С. 106266 - 106266

Опубликована: Март 1, 2025

Язык: Английский

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Immunomodulation of Glycyrrhiza Polysaccharides In Vivo Based on Microbiome and Metabolomics Approaches

Foods, Год журнала: 2025, Номер 14(5), С. 874 - 874

Опубликована: Март 4, 2025

Glycyrrhiza uralensis Fisch. is a medicinal herb that can be added to food provide therapeutic effects and reduce the burden of medications. Herein, immunomodulatory polysaccharides (GPs) were verified illustrated by intervening immunocompromised rats treated with different doses GPs, which reflected for adjusting composition structure intestinal microbiota altering metabolic profile. The GPs exerted regulating microenvironment. In particular, could promote growth probiotic bacteria Allobaculum, norank__o_Clostridia_UCG-014, Dubosiella, g__norank_o___RF39 curb harmful Enterococcus. results showed had prebiotic effect, contributed improving environment maintaining health. addition, content beneficial differential metabolites was up-regulated, especially short-chain fatty acids, alanine, aspartate, glutamate metabolism; arginine biosynthesis; glyoxylate dicarboxylate metabolism being most enriched pathways. These pathways imply process effector it are focused. Overall, purpose this article lies in providing support application immune function.

Язык: Английский

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The role of phosphatidylcholine 34:1 in the occurrence, development and treatment of ulcerative colitis

Acta Pharmaceutica Sinica B, Год журнала: 2022, Номер 13(3), С. 1231 - 1245

Опубликована: Сен. 13, 2022

Lipid homeostasis is considered to be related intestinal metabolic balance, while its role in the pathogenesis and treatment of ulcerative colitis (UC) remains largely unexplored. The present study aimed identify target lipids occurrence, development UC by comparing lipidomics patients, mice colonic organoids with corresponding healthy controls. Here, multi-dimensional based on LC-QTOF/MS, LC-MS/MS iMScope systems were constructed used decipher alteration lipidomic profiles. results indicated that patients often accompanied dysregulation lipid homeostasis, which triglycerides phosphatidylcholines significantly reduced. Notably, phosphatidylcholine 34:1 (PC34:1) was characterized high abundance closely correlation disease. Our also revealed down-regulation PC synthase PCYT1α Pemt caused modeling main factor leading reduction PC34:1, exogenous PC34:1 could greatly enhance fumarate level via inhibiting transformation glutamate N-acetylglutamate, thus exerting an anti-UC effect. Collectively, our not only supplies common technologies strategies for exploring metabolism mammals, but provides opportunities discovery therapeutic agents biomarkers UC.

Язык: Английский

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Potent Intestinal Mucosal Barrier Enhancement of Nostoc commune Vaucher Polysaccharide Supplementation Ameliorates Acute Ulcerative Colitis in Mice Mediated by Gut Microbiota

Nutrients, Год журнала: 2023, Номер 15(13), С. 3054 - 3054

Опубликована: Июль 6, 2023

Ulcerative colitis (UC) is evolving into a global burden with substantially increasing incidence in developing countries. It characterized by inflammation confined to mucosa and recognized as an intestinal barrier disease. The microbiota plays crucial role UC pathogenesis. N. commune has long been appreciated healthy food supplement worldwide polysaccharides account for 60%. Here, we examined the amelioration of against acute mice induced DSS assessed mediating gut microbiota. An integrated analysis microbiome, metabolomics, transcriptomics fully elaborated it markedly enhanced mucosal function, including: relative abundance Akkermansia muciniphila, uncultured_bacterium_g__norank_f__Muribaculaceae, unclassified_g__norank_f__norank_o__Clostridia_UCG-014; decreasing microbiota-derived phosphatidylcholines thromboxane 2 levels mapped arachidonic acid metabolism; improving mucin2 biosynthesis secretion; enhancing ZO-1 occludin expression; reducing neutrophil infiltration; regulating level colitis-related inflammatory cytokines; involving immune function-associated signaling pathways. Further, mediation effect was evaluated administering cocktail antibiotics. In conclusion, our results demonstrated that predominantly reinforced microbiota-mediated confer protection exhibited dramatic prebiotic-like functions, providing alternative or complementary treatment UC.

Язык: Английский

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Rectal microbiomes and serum metabolomics reveal the improved effect of Artemisia ordosica crude polysaccharides on the lactation performance, antioxidant status, and immune responses of lactating donkeys

Journal of Dairy Science, Год журнала: 2024, Номер 107(9), С. 6696 - 6716

Опубликована: Апрель 11, 2024

This study is aimed at investigating the effects of dietary supplementation with Artemisia ordosica crude polysaccharides (AOCP) on lactation performance, antioxidant status, and immune status lactating donkeys analyzing rectal microbiomes serum metabolomes. Fourteen Dezhou similar age (6.16 ± 0.67 yr BW SD), weight (250.06 25.18 kg), DIM (39.11 7.42 d), average parity 3 were randomly allocated into 2 treatments: a control group (CON, basal diet) an AOCP (AOCP, diet 1.0 g/kg DM AOCP). Ten weeks allotted for experiment, wk adaptation, 8 collecting data samples. The results showed that donkey diets increased including DMI, milking yield, estimated milk solids-corrected milk, ECM, fat protein lactose TS SNF yield. digestibility DM, CP, ADF, NDF was in compared CON group. concentrations IgA, IgG, IgM, activities superoxide dismutase, catalase, total capacity serum. decreased tumor necrosis factor-α, nitric oxide, reactive oxygen species, malondialdehyde Compared group, propionate, butyrate, isovalerate, VFA feces (P < 0.05). addition to diversity (Shannon index) altered structure microflora. As result supplementation, there has been significant improvement colonization beneficial bacteria, Lactobacillus, Unclassified_f_Prevotellacea, Ruminococcus, Fibrobacter genera. In contrast, decrease Clostridium_sensu_stricto_1 bacterial genus other pathogenic bacteria observed. Meanwhile, metabolomics analysis found upregulated metabolites l-tyrosine content while downregulating 9(S)-HODE, choline, sucrose, lysophosphatidylcholine (LysoPC) (18:0), LysoPC (18:1(9Z)), (20:2(11Z,14Z)) concentrations. These involved PPAR signaling pathway, prolactin glycerophospholipid metabolism, carbohydrate digestion absorption, tyrosine metabolism pathways, which mainly related capacity, responses, donkeys. consequence feeding diets, abundant, metabolism-related pathways enriched, may enhance performance Therefore, supplementing desirable strategy improve health performance.

Язык: Английский

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Eisenia bicyclis Extract Ameliorates Colitis in In Vitro and In Vivo Models Through Modulation of mTOR Axis and Gut Microbiota Composition

Foods, Год журнала: 2025, Номер 14(5), С. 714 - 714

Опубликована: Фев. 20, 2025

Ulcerative colitis (UC) is a chronic inflammatory disease of the colon that associated with dysbiosis in gut microbiota. Eisenia bicyclis, marine alga, known for its anti-inflammatory, antioxidant, and microbiota-modulating properties. This study explored mechanisms by which 70% ethanol extract E. bicyclis may alleviate UC, through both vitro vivo experiments. LC-MS/MS analysis revealed eckol, 7-phloroeckol, dieckol, phlorofucofuroeckol A, fucofuroeckol as key phenolic compounds present extract. The administration significantly improved symptoms dextran sulfate sodium (DSS)-induced mouse model reducing intestinal shortening, splenomegaly, histological scores. Both cell animal studies demonstrated suppressed release cytokines, downregulated mRNA expression genes related to mTOR pathway, reduced p-mTOR/mTOR ratio. Microbiota that, while Firmicutes/Bacteroidetes ratio was elevated UC mice, normalized this imbalance, notable increase abundance beneficial probiotics such Bifidobacterium bifidum. In conclusion, phenolic-rich demonstrates significant potential dietary supplement prevent mitigate modulating signaling pathway microbiota composition.

Язык: Английский

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