LWT,
Год журнала:
2024,
Номер
206, С. 116598 - 116598
Опубликована: Авг. 1, 2024
Eicosapentaenoic
acid
(EPA),
an
orally
active
Omega-3
long-chain
polyunsaturated
fatty
acid,
has
attracted
much
attention
for
its
medical
and
healthcare
value.
Due
to
a
large
amount
of
acids
(PUFA)
including
docosahexanoic
(DHA)
in
fish
oil,
the
highly
specific
enrichment
EPA
from
oil
is
difficult
achieve.
In
our
study,
we
conducted
immobilization
studies
on
Burkholderia
cepacia
lipase
(BCL)
application
selective
oil.
BCL
was
immobilized
resin
LX201A
by
covalent
crosslinking,
conditions
glutaraldehyde
concentration,
pH,
time
were
optimized.
The
optimal
0.5%
glutaraldehyde,
4
h,
pH
8.0.
loading
rate
reached
93.5%,
activity
up
68263
U/g
under
conditions.
After
hydrolysis
reaction
15h
catalyzed
BCL,
content
showed
more
increase
33.2%,
while
slight
0.78%
DHA
content.
Moreover,
after
recycling
use
10
times,
catalytic
efficiency
remained
at
93.0%.
These
indicated
that
possessed
good
selectivity,
reusability
stability,
providing
great
potential
industry.
Journal of Composites Science,
Год журнала:
2023,
Номер
7(12), С. 488 - 488
Опубликована: Ноя. 26, 2023
For
over
a
century,
enzyme
immobilisation
has
been
proven
to
be
superior
strategy
improve
catalytic
activity
and
reusability
ensure
easy
separation,
operation,
reduced
cost.
Enzyme
allows
for
an
easier
separation
of
the
from
reaction
mixture,
thus
simplifying
downstream
processing.
This
technology
protects
degradation
or
inactivation
by
harsh
conditions,
making
it
more
robust
suitable
used
in
various
applications.
Recent
strategies
methods,
such
as
adsorption,
cross-linking,
entrapment
encapsulation,
covalent
bonding,
were
critically
reviewed.
These
have
shown
promising
results
improving
stability,
activity,
A
recent
development
nanomaterials
agrowaste
renewable
carriers
is
underlined
current
review.
Furthermore,
use
gained
significant
attention
due
their
unique
properties,
high
surface
area,
mass
transfer,
biocompatibility,
sustainability.
materials
offer
outcomes
developing
efficient
sustainable
immobilised
enzymes.
state-of-the-art
better
control
reactions
enhances
reusability,
leading
cost-effective
environmentally
friendly
processes.
The
also
helps
reduce
waste
generation
promote
utilisation
resources,
further
contributing
circular
economy.
Chemical Communications,
Год журнала:
2023,
Номер
59(49), С. 7518 - 7533
Опубликована: Янв. 1, 2023
This
review
summarises
the
use
of
engineered
ketoreductases
(KREDS),
both
as
whole
microbial
cells
and
isolated
enzymes,
in
highly
enantiospecific
reduction
prochiral
ketones.
ACS Omega,
Год журнала:
2024,
Номер
9(47), С. 46698 - 46732
Опубликована: Ноя. 14, 2024
Immobilization
of
lipases
by
physical
adsorption
improves
their
stability,
recovery,
and
reusability
in
biotechnological
processes.
The
present
review
provides
an
advanced
bibliometric
analysis
a
comprehensive
overview
research
progress
this
field.
By
searching
Web
Science,
39,575
publications
were
analyzed,
325
relevant
articles
selected.
Key
journals,
countries,
institutions,
authors
identified.
most
cited
focus
on
biofuel
production
industrial
applications.
revealed
four
themes
with
the
biofuel.
method
is
effective
when
appropriate
support
used.
Despite
decrease
patent
applications,
interest
remains
high.
Future
studies
should
optimizing
materials
exploring
new
applications
technique.
detailed
understanding
immobilization
adsorption.
