Immobilization of Nitrilase: Driving Efficiency and Sustainability in Pharmaceutical Synthesis DOI
Rahul Vikram Singh

ChemBioEng Reviews, Год журнала: 2025, Номер unknown

Опубликована: Апрель 15, 2025

Abstract Enzyme immobilization has propelled the pharmaceutical sector forward by playing a critical role in chemical synthesis; therefore, biocatalyst reuse industries received significant attention due to its eco‐friendly nature and potential for streamlining future utilization. In particular, nitrilases have emerged as biocatalysts synthesizing intermediates active ingredients (APIs). created platform nitrilase that significantly improved thermal stability reusability, increasing commercial value industry. The current article provides comprehensive analysis of recent advances continuous flow systems, focusing on how this approach contributes efficiency sustainability exploitation advantages immobilization, such stability, ease isolation, along with biocatalysts, proven be an invaluable tool pursuit more environment‐friendly efficient synthesis.

Язык: Английский

Global Advancements in Bioactive Material Manufacturing for Drug Delivery: A Comprehensive Study DOI Creative Commons
Rafael Leandro Fernandes Melo, Dayana Nascimento Dari, Francisco Izaias da Silva Aires

и другие.

ACS Omega, Год журнала: 2025, Номер 10(1), С. 1207 - 1225

Опубликована: Янв. 3, 2025

Manufacturing bioactive materials for drug delivery involves developing that interact with biological tissues to release drugs in a controlled and targeted manner. The goal is optimize therapeutic efficacy reduce side effects by combining knowledge from engineering, biology, pharmacology. This study presents detailed bibliometric analysis, exploring the keywords "manufacturing," "bioactive materials," "drug delivery" identify highlight significant advancements field. From Web of Science, 36,504 articles were analyzed, 171 selected deeper identifying key journals, countries, institutions, authors. results field's interdisciplinary nature, grouped into four main themes, including regenerative medicine, scaffolds, three-dimensional (3D) printing, glass, tissue engineering. Future research this area will focus on more effective precise systems using technologies like 3D printing nanotechnology enhance customization control release, aiming efficient therapies.

Язык: Английский

Процитировано

3

Advancements and Prospects in Nanorobotic Applications for Ophthalmic Therapy DOI Creative Commons
Achilles Nina Santos Ferreira, John Hebert da Silva Félix, Rita Karolinny Chaves de Lima

и другие.

ACS Biomaterials Science & Engineering, Год журнала: 2025, Номер unknown

Опубликована: Янв. 17, 2025

This study provides a bibliometric and bibliographic review of emerging applications micro- nanotechnology in treating ocular diseases, with primary focus on glaucoma. We aim to identify key research trends analyze advancements devices drug delivery systems for treatments. The methodology involved analyzing 385 documents indexed the Web Science using tools such as VOSviewer Bibliometrix. results show marked increase scientific output, highlighting prominent authors institutions, England leading field. Key findings suggest that holds potential address limitations conventional treatments, including low bioavailability adverse side effects. Nanoparticles, nanovesicles, polymer-based appear promising prolonged controlled release, potentially offering enhanced therapeutic efficacy. In conclusion, could transform disease treatment, although challenges remain concerning biocompatibility scalability these devices. Further clinical studies are necessary establish innovations within context ophthalmology.

Язык: Английский

Процитировано

2

Characterization of a purified novel Aureobasidium pullulans NAC8 lipase and covalent-immobilization for use in the biodegradation of oil-contaminated wastewater DOI
Adedeji Nelson Ademakinwa, Zainab Adenike Ayinla,

Agunbiade Mayowa Oladele

и другие.

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 140781 - 140781

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

1

Heterologous expression and enzymatic properties of lipase from Mucor circinelloides DOI Creative Commons
Yao Zhang, Yan Sun, Zhuo Liu

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Март 29, 2025

Microbial lipases could be used to hydrolyze or recombine fats and oils, had great applications in food processing, bioenergy chemical industry. Mucor circinelloides was an important gamma-linolenic acid producing strain, its genome predicted contain a large number of genes encoding lipases, the key enzymes lipid metabolism. In present study, potential lipase WJ_23 from WJ11 cloned for first time heterologously expressed purified homogeneity Pichia pastoris. By SDS-PAGE analysis, molecular weight recombinant estimated ~ 34 kDa. The optimal temperature pH were 50 °C 9.0, respectively. good thermal stability at with broad 6.0 11.0. After incubation 37 24 h, activity remained over 95% between 7.0 9.0. possessed preference long chain substrates. Using p-NPP as substrate, measured kinetic parameters Vmax Km 94.34 U/mg 6.37 mmoL/L, addition, not affected obviously by various metal ions, it exhibited certain tolerance towards several kinds organic solvents. This research might provide basis further industrial application M. lipase.

Язык: Английский

Процитировано

0

Enzymatic conversion of glycerol to dihydroxyacetone using a hydrogel bioreactor entrapping glycerol dehydrogenase immobilized on silica nanoparticles DOI

W Oh,

Jeongwoo Ham,

Se‐Min Kim

и другие.

Chemical Engineering Journal, Год журнала: 2025, Номер 505, С. 159250 - 159250

Опубликована: Янв. 8, 2025

Язык: Английский

Процитировано

0

Sustainable Alternative Media for the Production of Lipolytic Cells and Fatty Acid Concentrates: Integration of the Enzyme and Food Industries DOI Creative Commons
Willian de Souza Matias Reis, Arthur Ouro Preto, Giovana de Oliveira Santana

и другие.

Foods, Год журнала: 2025, Номер 14(6), С. 990 - 990

Опубликована: Март 14, 2025

The use of agro-industrial by-products and processing residues, which are rich in carbohydrates, proteins, lipids, the production lipases allows sustainable these reducing environmental impacts. In this study, immersion water lentils, soybeans, textured soy protein was evaluated as carbon nitrogen sources whole-cell lipases, resulting biomass used hydrolysis residual soybean oil with conventional heating ultrasound. results showed that best culture medium one 50% protein, reaching values 149.04 U/g hydrolytic activity, 12.92 g/L concentration, 144.17 U total specific volumetric productivities 2.07 U/g·h 20.02 U/L·h, respectively. positive effect adding frying to crop observed, increased cell activity. obtained potential for ultrasound-assisted vegetable oils, approximately 43.36% 7 h reaction, an initial rate 31.03 mmol/h. It is concluded a viable candidate replace traditional sources, being economically attractive alternative due its wide generation restaurants.

Язык: Английский

Процитировано

0

Advances and Applications of Micro- and Mesofluidic Systems DOI Creative Commons
Luís Alves, John Hebert da Silva Félix, Achilles Nina Santos Ferreira

и другие.

ACS Omega, Год журнала: 2025, Номер unknown

Опубликована: Март 25, 2025

Microfabrication technology has advanced scientific understanding and expanded our molecular control capabilities, enabling the development of 3D models in micrometer structures. The sizes fluidic channels are arranged descending order, starting with macro-, followed by meso-, micro-, nanoscale. These advances bring advantages speed up biological chemical experimental processes. Such miniaturized systems show significant advances, particularly meso- microreactors, through high-throughput screening. This work proposes a bibliometric analysis applications Web Science (WoS) database, analyzing main highlights publications, indicators, impact on knowledge production. In past 20 years, approximately 3,934 documents published cited, mainly major world powers micro- mesofluidic systems, increasingly expanding academic industrial sectors.

Язык: Английский

Процитировано

0

Immobilization of Nitrilase: Driving Efficiency and Sustainability in Pharmaceutical Synthesis DOI
Rahul Vikram Singh

ChemBioEng Reviews, Год журнала: 2025, Номер unknown

Опубликована: Апрель 15, 2025

Abstract Enzyme immobilization has propelled the pharmaceutical sector forward by playing a critical role in chemical synthesis; therefore, biocatalyst reuse industries received significant attention due to its eco‐friendly nature and potential for streamlining future utilization. In particular, nitrilases have emerged as biocatalysts synthesizing intermediates active ingredients (APIs). created platform nitrilase that significantly improved thermal stability reusability, increasing commercial value industry. The current article provides comprehensive analysis of recent advances continuous flow systems, focusing on how this approach contributes efficiency sustainability exploitation advantages immobilization, such stability, ease isolation, along with biocatalysts, proven be an invaluable tool pursuit more environment‐friendly efficient synthesis.

Язык: Английский

Процитировано

0