LWT,
Год журнала:
2024,
Номер
206, С. 116598 - 116598
Опубликована: Авг. 1, 2024
Eicosapentaenoic
acid
(EPA),
an
orally
active
Omega-3
long-chain
polyunsaturated
fatty
acid,
has
attracted
much
attention
for
its
medical
and
healthcare
value.
Due
to
a
large
amount
of
acids
(PUFA)
including
docosahexanoic
(DHA)
in
fish
oil,
the
highly
specific
enrichment
EPA
from
oil
is
difficult
achieve.
In
our
study,
we
conducted
immobilization
studies
on
Burkholderia
cepacia
lipase
(BCL)
application
selective
oil.
BCL
was
immobilized
resin
LX201A
by
covalent
crosslinking,
conditions
glutaraldehyde
concentration,
pH,
time
were
optimized.
The
optimal
0.5%
glutaraldehyde,
4
h,
pH
8.0.
loading
rate
reached
93.5%,
activity
up
68263
U/g
under
conditions.
After
hydrolysis
reaction
15h
catalyzed
BCL,
content
showed
more
increase
33.2%,
while
slight
0.78%
DHA
content.
Moreover,
after
recycling
use
10
times,
catalytic
efficiency
remained
at
93.0%.
These
indicated
that
possessed
good
selectivity,
reusability
stability,
providing
great
potential
industry.
Industrial & Engineering Chemistry Research,
Год журнала:
2023,
Номер
62(39), С. 15733 - 15751
Опубликована: Сен. 20, 2023
The
use
of
biocatalysts
in
pharmaceutical
production
is
an
important
approach
for
the
industry
to
achieve
green
manufacturing.
Compared
other
lipases,
Candida
antarctica
lipase
B
(CALB)
possesses
numerous
excellent
characteristics
due
its
unique
structure.
It
exhibits
relatively
strong
catalytic
activity
toward
both
water-insoluble
and
water-soluble
substances.
shows
high
selectivity
hydrolysis
organic
synthesis
reactions.
These
properties
are
crucial
drug
synthesis.
However,
current
research
that
CALB
as
a
catalyst
still
faces
challenges
such
cost,
poor
stability,
difficulty
large-scale
production.
Therefore,
this
paper
mainly
explores
advantages
need
be
overcome.
introduces
application
progress
finally
provides
prospect
future
directions,
hoping
provide
appropriate
references
related
research.
Molecules,
Год журнала:
2023,
Номер
28(3), С. 1305 - 1305
Опубликована: Янв. 30, 2023
Cross-linked
enzyme
aggregates
(CLEAs)
represent
an
effective
tool
for
carrier-free
immobilization
of
enzymes.
The
present
study
promotes
a
successful
application
functionalized
magnetic
nanoparticles
(MNPs)
stabilization
cellulase
CLEAs.
Catalytically
active
CLEAs
and
cross-linked
(mCLEAs)
from
Trichoderma
reesei
were
prepared
using
glutaraldehyde
(GA)
as
cross-linking
agent
the
catalytic
activity
stability
CLEAs/mCLEAs
investigated.
influence
precipitation
agents,
cross-linker
concentration,
concentration
enzyme,
addition
bovine
serum
albumin
(BSA),
sodium
cyanoborohydride
(NaBH3CN)
on
expressed
yield
was
studied.
Particularly,
reducing
unsaturated
Schiff’s
base
to
form
irreversible
linkages
is
important
improved
(86%)
mCLEAs
(91%).
For
increased
applicability
CLEAs/mCLEAs,
we
enhanced
at
mild
biochemical
process
conditions.
reusability
after
10
cycles
both
investigated,
which
retained
72%
65%
initial
activity,
respectively.
thermal
in
comparison
with
non-immobilized
obtained
30
°C
(145.65%
188.7%,
respectively)
50
(185.1%
141.4%,
respectively).
Kinetic
parameters
determined
mCLEAs,
KM
constant
found
0.055
±
0.0102
mM
0.037
0.0012
mM,
maximum
velocity
rate
(Vmax)
calculated
1.12
µmol/min
CLEA
1.17
0.0023
mCLEA.
Structural
characterization
studied
XRD,
SEM,
FT-IR.
Catalytical
properties
immobilized
reducent
NaBH3CN
by
enhancing
aminosilane
MNPs
mCLEAs.
LWT,
Год журнала:
2024,
Номер
206, С. 116598 - 116598
Опубликована: Авг. 1, 2024
Eicosapentaenoic
acid
(EPA),
an
orally
active
Omega-3
long-chain
polyunsaturated
fatty
acid,
has
attracted
much
attention
for
its
medical
and
healthcare
value.
Due
to
a
large
amount
of
acids
(PUFA)
including
docosahexanoic
(DHA)
in
fish
oil,
the
highly
specific
enrichment
EPA
from
oil
is
difficult
achieve.
In
our
study,
we
conducted
immobilization
studies
on
Burkholderia
cepacia
lipase
(BCL)
application
selective
oil.
BCL
was
immobilized
resin
LX201A
by
covalent
crosslinking,
conditions
glutaraldehyde
concentration,
pH,
time
were
optimized.
The
optimal
0.5%
glutaraldehyde,
4
h,
pH
8.0.
loading
rate
reached
93.5%,
activity
up
68263
U/g
under
conditions.
After
hydrolysis
reaction
15h
catalyzed
BCL,
content
showed
more
increase
33.2%,
while
slight
0.78%
DHA
content.
Moreover,
after
recycling
use
10
times,
catalytic
efficiency
remained
at
93.0%.
These
indicated
that
possessed
good
selectivity,
reusability
stability,
providing
great
potential
industry.
