Immobilized Burkholderia cepacia lipase and its application in selective enrichment of EPA by hydrolysis of fish oil

LWT, Journal Year: 2024, Volume and Issue: 206, P. 116598 - 116598

Published: Aug. 1, 2024

Eicosapentaenoic acid (EPA), an orally active Omega-3 long-chain polyunsaturated fatty acid, has attracted much attention for its medical and healthcare value. Due to a large amount of acids (PUFA) including docosahexanoic (DHA) in fish oil, the highly specific enrichment EPA from oil is difficult achieve. In our study, we conducted immobilization studies on Burkholderia cepacia lipase (BCL) application selective oil. BCL was immobilized resin LX201A by covalent crosslinking, conditions glutaraldehyde concentration, pH, time were optimized. The optimal 0.5% glutaraldehyde, 4 h, pH 8.0. loading rate reached 93.5%, activity up 68263 U/g under conditions. After hydrolysis reaction 15h catalyzed BCL, content showed more increase 33.2%, while slight 0.78% DHA content. Moreover, after recycling use 10 times, catalytic efficiency remained at 93.0%. These indicated that possessed good selectivity, reusability stability, providing great potential industry.

Language: Английский

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Development of a novel ultrasound- and biocrosslinking-enhanced immobilization strategy with application to food enzymes

Food Chemistry, Journal Year: 2023, Volume and Issue: 417, P. 135810 - 135810

Published: March 7, 2023

Language: Английский

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Application of Lipase B from Candida antarctica in the Pharmaceutical Industry

Industrial & Engineering Chemistry Research, Journal Year: 2023, Volume and Issue: 62(39), P. 15733 - 15751

Published: Sept. 20, 2023

The use of biocatalysts in pharmaceutical production is an important approach for the industry to achieve green manufacturing. Compared other lipases, Candida antarctica lipase B (CALB) possesses numerous excellent characteristics due its unique structure. It exhibits relatively strong catalytic activity toward both water-insoluble and water-soluble substances. shows high selectivity hydrolysis organic synthesis reactions. These properties are crucial drug synthesis. However, current research that CALB as a catalyst still faces challenges such cost, poor stability, difficulty large-scale production. Therefore, this paper mainly explores advantages need be overcome. introduces application progress finally provides prospect future directions, hoping provide appropriate references related research.

Language: Английский

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A review on the immobilization of bromelain

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 273, P. 133089 - 133089

Published: June 14, 2024

This review shows the endeavors performed to prepare immobilized formulations of bromelain extract, usually from pineapple, and their use in diverse applications. extract has a potent proteolytic component that is based on thiol proteases, which differ depending location fruit. Stem fruit are areas where higher activity found. The edible origin this enzyme one features determines applications more significant degree. been wide diversity supports via different strategies (covalent bonds, ion exchange), also forming ex novo solids (nanoflowers, CLEAs, trapping alginate beads, etc.). preexisting nanoparticles as immobilization relevant, facilitates main enzyme, therapeutic (as wound dressing healing components, antibacterial or anticancer, mucus mobility control, A curiosity spores probiotic microorganisms adsorption, order have perfect vivo compatibility. Other outstanding stabilization wine versus haze during storage, mainly when chitosan. Curiously, scarcely applied production bioactive peptides.

Language: Английский

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Innovations in packed-bed reactors utilizing immobilized lipase catalysts: A comprehensive technical and scientific review

Molecular Catalysis, Journal Year: 2025, Volume and Issue: 573, P. 114814 - 114814

Published: Jan. 5, 2025

Language: Английский

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