Fermentation, Год журнала: 2024, Номер 10(12), С. 604 - 604

Опубликована: Ноя. 26, 2024

Hydroxylation reaction is a significant source of structural diversity in natural products (NPs), playing crucial role improving the bioactivity, solubility, and stability product molecules. This review summarizes latest research progress field hydroxylation, focusing on several key hydroxylases involved biosynthesis NPs, including cytochrome P450 monooxygenases, α-ketoglutarate-dependent hydroxylases, flavin-dependent monooxygenases. These enzymes achieve selective hydroxylation modification various such as terpenoids, flavonoids, steroids, through different catalytic mechanisms. systematically recent advances amino acids, lipids, phenylpropanoids, demonstrating potential synthetic biology strategies constructing artificial biosynthetic pathways producing hydroxylated derivatives. Through metabolic engineering, enzyme genetic combined with intelligence-assisted technologies, series engineered strains have been successfully constructed for efficient production NPs their derivatives, achieving synthesis NPs. has provided new avenues drug development, functional food, biomaterial also offered ideas industrial these compounds. In future, integrating pathway design, directed evolution, dynamic regulation, intelligence technology expected to further expand application enzyme-catalyzed reactions green complex promoting heights.

Язык: Английский