Recent Advances and Challenges in the Production of Hydroxylated Natural Products Using Microorganisms DOI Creative Commons
Chang Sun,

Rumei Zeng,

Tianpeng Chen

et al.

Fermentation, Journal Year: 2024, Volume and Issue: 10(12), P. 604 - 604

Published: Nov. 26, 2024

Hydroxylation reaction is a significant source of structural diversity in natural products (NPs), playing crucial role improving the bioactivity, solubility, and stability product molecules. This review summarizes latest research progress field hydroxylation, focusing on several key hydroxylases involved biosynthesis NPs, including cytochrome P450 monooxygenases, α-ketoglutarate-dependent hydroxylases, flavin-dependent monooxygenases. These enzymes achieve selective hydroxylation modification various such as terpenoids, flavonoids, steroids, through different catalytic mechanisms. systematically recent advances amino acids, lipids, phenylpropanoids, demonstrating potential synthetic biology strategies constructing artificial biosynthetic pathways producing hydroxylated derivatives. Through metabolic engineering, enzyme genetic combined with intelligence-assisted technologies, series engineered strains have been successfully constructed for efficient production NPs their derivatives, achieving synthesis NPs. has provided new avenues drug development, functional food, biomaterial also offered ideas industrial these compounds. In future, integrating pathway design, directed evolution, dynamic regulation, intelligence technology expected to further expand application enzyme-catalyzed reactions green complex promoting heights.

Language: Английский

Modular cascade with engineered HpaB for efficient synthesis of hydroxytyrosol DOI
Wenkai Liu,

Xiu‐Xin Ren,

Lian Xu

et al.

Bioorganic Chemistry, Journal Year: 2025, Volume and Issue: 155, P. 108125 - 108125

Published: Jan. 4, 2025

Language: Английский

Citations

1
Cutting-edge Computational Approaches in Enzyme Design and Activity Enhancement DOI

Ruobin Sun,

Dan Wu, Pengcheng Chen

et al.

Biochemical Engineering Journal, Journal Year: 2024, Volume and Issue: 212, P. 109510 - 109510

Published: Sept. 26, 2024

Language: Английский

Citations

3
Cinnamic acid and its derivatives on dipeptidyl peptidase 4: structure-activity relationship and mechanism of inhibition DOI
Jiaying Li, Xiaoping Yang,

Chunhong Xiong

et al.

Food Bioscience, Journal Year: 2024, Volume and Issue: 62, P. 105076 - 105076

Published: Sept. 7, 2024

Language: Английский

Citations

2
Recent Advances and Challenges in the Production of Hydroxylated Natural Products Using Microorganisms DOI Creative Commons
Chang Sun,

Rumei Zeng,

Tianpeng Chen

et al.

Fermentation, Journal Year: 2024, Volume and Issue: 10(12), P. 604 - 604

Published: Nov. 26, 2024

Hydroxylation reaction is a significant source of structural diversity in natural products (NPs), playing crucial role improving the bioactivity, solubility, and stability product molecules. This review summarizes latest research progress field hydroxylation, focusing on several key hydroxylases involved biosynthesis NPs, including cytochrome P450 monooxygenases, α-ketoglutarate-dependent hydroxylases, flavin-dependent monooxygenases. These enzymes achieve selective hydroxylation modification various such as terpenoids, flavonoids, steroids, through different catalytic mechanisms. systematically recent advances amino acids, lipids, phenylpropanoids, demonstrating potential synthetic biology strategies constructing artificial biosynthetic pathways producing hydroxylated derivatives. Through metabolic engineering, enzyme genetic combined with intelligence-assisted technologies, series engineered strains have been successfully constructed for efficient production NPs their derivatives, achieving synthesis NPs. has provided new avenues drug development, functional food, biomaterial also offered ideas industrial these compounds. In future, integrating pathway design, directed evolution, dynamic regulation, intelligence technology expected to further expand application enzyme-catalyzed reactions green complex promoting heights.

Language: Английский

Citations

1