Yin Yang 1: Function, Mechanisms, and Glia
Neurochemical Research,
Год журнала:
2025,
Номер
50(2)
Опубликована: Фев. 4, 2025
Abstract
Yin
Yang
1
is
a
ubiquitously
expressed
transcription
factor
that
has
been
extensively
studied
given
its
particular
dual
transcriptional
regulation.
involved
in
various
cellular
processes
like
cell
cycle
progression,
differentiation,
DNA
repair,
survival
and
apoptosis
among
others.
Its
malfunction
or
alteration
leads
to
disease
even
malignant
transformation.
This
essential
for
the
proper
central
nervous
system
development
function.
The
activity
of
depends
on
interacting
partners,
promoter
environment
chromatin
structure,
however,
mechanistic
not
completely
understood.
In
this
review,
we
briefly
discuss
post-translational
modifications,
interactions,
functions
participation
neurodevelopment.
We
also
expression
critical
involvement
physiology
physiopathology
glial
cells,
summarizing
contribution
different
aspects
Язык: Английский
A panel of six immune-related mRNAs as biomarkers for tuberculosis diagnosis
Frontiers in Genetics,
Год журнала:
2025,
Номер
16
Опубликована: Март 20, 2025
Objective
This
study
aims
to
screen
common
immunological
markers
of
lung
tissues
and
blood
for
diagnosis
tuberculosis
(TB).
Methods
Differentially
expressed
miRNAs
(DEmRs)
mRNAs
(DEGs)
were
obtained
by
whole-transcriptome
sequencing
profiles
on
18F-FDG
PET/CT
high
low
metabolic
active
regions
in
nine
TB
patients.
Common
screened
intersecting
with
DEmRs,
four
miRNA
GEO
datasets,
their
target
predicted
through
the
miRTarbase
Tarbase
databases.
Then
these
intersected
DEGs,
from
samples
immune-related
genes,
construct
a
miRNA-mRNA
interaction
network,
hub
genes
identified
Cytoscape.
The
relationship
between
immune
infiltration
evaluated
using
Cibersort.
Finally,
diagnostic
model
based
Lasso
regression
analysis
was
established
validated
qRT-PCR.
Results
Five
both
tissues.
Six
(
NEDD4
,
PLTP
RNASEL
SEMA7A
TAPBP
THBS1
)
out.
A
30
pairs
(TB/health
volunteers).
AUC
6-mRNA
combination
0.79.
Conclusion
We
six
as
diagnosing
tuberculosis.
Язык: Английский
Identification and validation of CDC20 and ITCH as ubiquitination related biomarker in idiopathic pulmonary fibrosis
Hereditas,
Год журнала:
2025,
Номер
162(1)
Опубликована: Апрель 1, 2025
Abstract
Purpose
Ubiquitination
plays
a
crucial
role
in
various
diseases.
This
study
aims
to
explore
the
potential
ubiquitination
related
genes
IPF.
Methods
The
gene
microarray
dataset
GSE24206
was
obtained
from
GEO
database.
Subsequently,
through
differential
expression
analysis
and
molecular
signatures
database,
we
1734
differentially
expressed
742
genes.
Through
venn
diagram
analysis,
53
Then,
gene-ontology
(GO)
enrichment
Kyoto
Encyclopedia
of
Genes
Genomes
(KEGG)
pathway
protein-protein
interactions
(PPI)
set
(GSEA)
were
applied
for
Finally,
CDC20
ITCH
IPF
patients
cells
validated
by
qPCR
western
blot
assay.
Results
A
total
(36
up-regulated
17
down-regulated
genes)
identified
between
6
healthy
controls.
GO
KEGG
mainly
involved
regulation
protein
ubiquitination,
post-translational
modification
ubiquitin
mediated
proteolysis.
PPI
results
demonstrated
that
these
interacted
with
each
other.
GSEA
some
hub
epithelial
mesenchymal
transition,
inflammatory
response,
hypoxia,
apoptosis.
experiment
level
consistent
bioinformatics
results.
Conclusion
We
analysis.
other
may
influence
development
transition
response.
Our
research
findings
provide
insights
into
mechanisms
fibrosis
evidence
therapeutic
targets
fibrosis.
Язык: Английский
Combining proteomics and Phosphoproteomics to investigate radiation-induced rectal fibrosis in rats and the effects of pSTAT3 inhibitor S3I-201 on human intestinal fibroblasts
Hongfeng Pan,
Zeyi Zhao,
Yuanchang Zhu
и другие.
Journal of Proteomics,
Год журнала:
2024,
Номер
308, С. 105287 - 105287
Опубликована: Авг. 23, 2024
Язык: Английский
Pathogenesis and Therapy of Hermansky–Pudlak Syndrome (HPS)-Associated Pulmonary Fibrosis
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(20), С. 11270 - 11270
Опубликована: Окт. 19, 2024
Hermansky–Pudlak
syndrome
(HPS)-associated
pulmonary
fibrosis
(HPS-PF)
is
a
progressive
lung
disease
that
major
cause
of
morbidity
and
mortality
in
HPS
patients.
Previous
studies
have
demonstrated
the
proteins
play
an
essential
role
biogenesis
function
lysosome-related
organelles
(LROs)
alveolar
epithelial
type
II
(AT2)
cells
found
HPS-PF
associated
with
dysfunction
AT2
abnormal
immune
reactions.
Despite
recent
advances
research
on
pathology
HPS-PF,
pathological
mechanisms
underlying
remain
poorly
understood,
no
effective
treatment
has
been
established.
Therefore,
it
necessary
to
refresh
progress
pathogenesis
increase
our
understanding
pathogenic
mechanism
develop
targeted
therapeutic
strategies.
This
review
summarizes
provides
information
about
current
strategies
for
hopefully
increases
offers
thoughts
new
interventions.
Язык: Английский
NEDD4L Inhibits the Proliferation and Migration of Keloid Fibroblasts by Regulating YY1 Ubiquitination‐Mediated Glycolytic Metabolic Reprogramming
Experimental Dermatology,
Год журнала:
2024,
Номер
33(11)
Опубликована: Ноя. 1, 2024
ABSTRACT
Keloid
scarring
is
a
complex
fibroproliferative
disorder
characterised
by
excessive
fibroblast
proliferation.
Inhibition
of
cellular
glycolysis
effectively
suppresses
the
proliferation
keloid
fibroblasts
(KFs).
Neural
precursor
cell‐expressed
developmentally
downregulated
gene
4‐like
(NEDD4L),
ubiquitin
ligase,
regulates
cell
in
different
diseases.
This
study
investigated
effects
NEDD4L
on
glucose
metabolism,
and
migration
KFs.
Primary
KFs
were
isolated
from
skin
tissues
obtained
patients
with
active‐stage
keloids.
Cell
transfection
was
used
to
upregulate
or
downregulate
Yin
Yang
1
(YY1)
Protein
expression
assessed
immunohistochemistry
western
blotting.
The
viability,
proliferative
capacity
ability
evaluated
using
MTT
method
EdU
wound
healing
assays,
respectively.
regulatory
effect
YY1
ubiquitination
examined
coimmunoprecipitation.
interaction
between
hexokinase
2
(HK2)
confirmed
dual‐luciferase
reporter
assay.
downregulated,
whereas
HK2
highly
expressed
compared
normal
skin.
Overexpression
inhibited
promoted
degradation
inducing
its
ubiquitination.
Upregulation
induced
consumption
lactate
production
via
transcriptional
regulation
HK2.
Increased
reversed
reduced
proliferation,
overexpressing
NEDD4L.
also
NEDD4L‐induced
inhibition
Additionally,
an
vivo
inhibitory
roles
overexpression
knockdown
formation.
suppressed
regulating
ubiquitination‐mediated
through
These
findings
suggest
novel
axis,
NEDD4L/YY1/HK2,
that
mediates
metabolism
Язык: Английский