NEDD4L Inhibits the Proliferation and Migration of Keloid Fibroblasts by Regulating YY1 Ubiquitination‐Mediated Glycolytic Metabolic Reprogramming DOI
Jun Jin, Kai Wang,

Chenxi Lu

et al.

Experimental Dermatology, Journal Year: 2024, Volume and Issue: 33(11)

Published: Nov. 1, 2024

ABSTRACT Keloid scarring is a complex fibroproliferative disorder characterised by excessive fibroblast proliferation. Inhibition of cellular glycolysis effectively suppresses the proliferation keloid fibroblasts (KFs). Neural precursor cell‐expressed developmentally downregulated gene 4‐like (NEDD4L), ubiquitin ligase, regulates cell in different diseases. This study investigated effects NEDD4L on glucose metabolism, and migration KFs. Primary KFs were isolated from skin tissues obtained patients with active‐stage keloids. Cell transfection was used to upregulate or downregulate Yin Yang 1 (YY1) Protein expression assessed immunohistochemistry western blotting. The viability, proliferative capacity ability evaluated using MTT method EdU wound healing assays, respectively. regulatory effect YY1 ubiquitination examined coimmunoprecipitation. interaction between hexokinase 2 (HK2) confirmed dual‐luciferase reporter assay. downregulated, whereas HK2 highly expressed compared normal skin. Overexpression inhibited promoted degradation inducing its ubiquitination. Upregulation induced consumption lactate production via transcriptional regulation HK2. Increased reversed reduced proliferation, overexpressing NEDD4L. also NEDD4L‐induced inhibition Additionally, an vivo inhibitory roles overexpression knockdown formation. suppressed regulating ubiquitination‐mediated through These findings suggest novel axis, NEDD4L/YY1/HK2, that mediates metabolism

Language: Английский

Yin Yang 1: Function, Mechanisms, and Glia DOI Creative Commons
Ada G. Rodríguez-Campuzano, Francisco Castelán, Luisa C. Hernández‐Kelly

et al.

Neurochemical Research, Journal Year: 2025, Volume and Issue: 50(2)

Published: Feb. 4, 2025

Abstract Yin Yang 1 is a ubiquitously expressed transcription factor that has been extensively studied given its particular dual transcriptional regulation. involved in various cellular processes like cell cycle progression, differentiation, DNA repair, survival and apoptosis among others. Its malfunction or alteration leads to disease even malignant transformation. This essential for the proper central nervous system development function. The activity of depends on interacting partners, promoter environment chromatin structure, however, mechanistic not completely understood. In this review, we briefly discuss post-translational modifications, interactions, functions participation neurodevelopment. We also expression critical involvement physiology physiopathology glial cells, summarizing contribution different aspects

Language: Английский

Citations

0
A panel of six immune-related mRNAs as biomarkers for tuberculosis diagnosis DOI Creative Commons

Yutong Wei,

Zilu Wen,

Qinghua Xue

et al.

Frontiers in Genetics, Journal Year: 2025, Volume and Issue: 16

Published: March 20, 2025

Objective This study aims to screen common immunological markers of lung tissues and blood for diagnosis tuberculosis (TB). Methods Differentially expressed miRNAs (DEmRs) mRNAs (DEGs) were obtained by whole-transcriptome sequencing profiles on 18F-FDG PET/CT high low metabolic active regions in nine TB patients. Common screened intersecting with DEmRs, four miRNA GEO datasets, their target predicted through the miRTarbase Tarbase databases. Then these intersected DEGs, from samples immune-related genes, construct a miRNA-mRNA interaction network, hub genes identified Cytoscape. The relationship between immune infiltration evaluated using Cibersort. Finally, diagnostic model based Lasso regression analysis was established validated qRT-PCR. Results Five both tissues. Six ( NEDD4 , PLTP RNASEL SEMA7A TAPBP THBS1 ) out. A 30 pairs (TB/health volunteers). AUC 6-mRNA combination 0.79. Conclusion We six as diagnosing tuberculosis.

Language: Английский

Citations

0
Identification and validation of CDC20 and ITCH as ubiquitination related biomarker in idiopathic pulmonary fibrosis DOI Creative Commons

Shulei Sun,

Yubao Wang,

Jing Feng

et al.

Hereditas, Journal Year: 2025, Volume and Issue: 162(1)

Published: April 1, 2025

Abstract Purpose Ubiquitination plays a crucial role in various diseases. This study aims to explore the potential ubiquitination related genes IPF. Methods The gene microarray dataset GSE24206 was obtained from GEO database. Subsequently, through differential expression analysis and molecular signatures database, we 1734 differentially expressed 742 genes. Through venn diagram analysis, 53 Then, gene-ontology (GO) enrichment Kyoto Encyclopedia of Genes Genomes (KEGG) pathway protein-protein interactions (PPI) set (GSEA) were applied for Finally, CDC20 ITCH IPF patients cells validated by qPCR western blot assay. Results A total (36 up-regulated 17 down-regulated genes) identified between 6 healthy controls. GO KEGG mainly involved regulation protein ubiquitination, post-translational modification ubiquitin mediated proteolysis. PPI results demonstrated that these interacted with each other. GSEA some hub epithelial mesenchymal transition, inflammatory response, hypoxia, apoptosis. experiment level consistent bioinformatics results. Conclusion We analysis. other may influence development transition response. Our research findings provide insights into mechanisms fibrosis evidence therapeutic targets fibrosis.

Language: Английский

Citations

0
Combining proteomics and Phosphoproteomics to investigate radiation-induced rectal fibrosis in rats and the effects of pSTAT3 inhibitor S3I-201 on human intestinal fibroblasts DOI

Hongfeng Pan,

Zeyi Zhao,

Yuanchang Zhu

et al.

Journal of Proteomics, Journal Year: 2024, Volume and Issue: 308, P. 105287 - 105287

Published: Aug. 23, 2024

Language: Английский

Citations

0
Pathogenesis and Therapy of Hermansky–Pudlak Syndrome (HPS)-Associated Pulmonary Fibrosis DOI Open Access
Xiao Mei Hu, Zhixiao Wei,

Yumeng Wu

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(20), P. 11270 - 11270

Published: Oct. 19, 2024

Hermansky–Pudlak syndrome (HPS)-associated pulmonary fibrosis (HPS-PF) is a progressive lung disease that major cause of morbidity and mortality in HPS patients. Previous studies have demonstrated the proteins play an essential role biogenesis function lysosome-related organelles (LROs) alveolar epithelial type II (AT2) cells found HPS-PF associated with dysfunction AT2 abnormal immune reactions. Despite recent advances research on pathology HPS-PF, pathological mechanisms underlying remain poorly understood, no effective treatment has been established. Therefore, it necessary to refresh progress pathogenesis increase our understanding pathogenic mechanism develop targeted therapeutic strategies. This review summarizes provides information about current strategies for hopefully increases offers thoughts new interventions.

Language: Английский

Citations

0
NEDD4L Inhibits the Proliferation and Migration of Keloid Fibroblasts by Regulating YY1 Ubiquitination‐Mediated Glycolytic Metabolic Reprogramming DOI
Jun Jin, Kai Wang,

Chenxi Lu

et al.

Experimental Dermatology, Journal Year: 2024, Volume and Issue: 33(11)

Published: Nov. 1, 2024

ABSTRACT Keloid scarring is a complex fibroproliferative disorder characterised by excessive fibroblast proliferation. Inhibition of cellular glycolysis effectively suppresses the proliferation keloid fibroblasts (KFs). Neural precursor cell‐expressed developmentally downregulated gene 4‐like (NEDD4L), ubiquitin ligase, regulates cell in different diseases. This study investigated effects NEDD4L on glucose metabolism, and migration KFs. Primary KFs were isolated from skin tissues obtained patients with active‐stage keloids. Cell transfection was used to upregulate or downregulate Yin Yang 1 (YY1) Protein expression assessed immunohistochemistry western blotting. The viability, proliferative capacity ability evaluated using MTT method EdU wound healing assays, respectively. regulatory effect YY1 ubiquitination examined coimmunoprecipitation. interaction between hexokinase 2 (HK2) confirmed dual‐luciferase reporter assay. downregulated, whereas HK2 highly expressed compared normal skin. Overexpression inhibited promoted degradation inducing its ubiquitination. Upregulation induced consumption lactate production via transcriptional regulation HK2. Increased reversed reduced proliferation, overexpressing NEDD4L. also NEDD4L‐induced inhibition Additionally, an vivo inhibitory roles overexpression knockdown formation. suppressed regulating ubiquitination‐mediated through These findings suggest novel axis, NEDD4L/YY1/HK2, that mediates metabolism

Language: Английский

Citations

0