Comparative transcriptomics ofLathyrus sativusreveals accession-specific resistance responses againstErysiphe pisi DOI Creative Commons
Ricardo Marques, Telma Fernandes, Pedro M. Barros

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 25, 2024

Lathyrus sativus (grass pea) is a valuable crop for sustainable agriculture, offering both dietary benefits and desirable agronomic traits. However, its yield stability limited by different diseases such as powdery mildew caused Erysiphe pisi . Frequent fungal resistance to pesticides growing environmental concerns highlight the need research investment develop resistant varieties. Four L. accessions, exhibiting varying levels of E. (resistant, partially resistant, susceptible, susceptible), were analysed using dual RNA-seq identify key defence mechanisms effector genes involved in this plant-pathogen interaction. The transcriptomic analysis highlighted host biphasic response, characterised an initial burst gene expression, followed quiescent phase, second wave intense expression at 72 hours after inoculation. Common mechanisms, including antifungal protein cell wall reinforcement, reactive oxygen species-mediated activated all accessions compared susceptible accession. Unique responses accession integrate early reinforcement structural barriers with sustained chemical defences stress responses. Overall, exhibited front-loaded focused on biotic stimuli interspecies interactions infection stages. In contrast, partial weaker baseline system, slower less robust response specifically targeting pathogen infection. We identified potential effectors, hydrolysis, nutrient acquisition, virulence, higher diversity effectors This study identifies novel targets within complex sativus-Erysiphe interaction that will support future breeding programs aimed enhancing other related species.

Язык: Английский

Lignin-based support for metal catalysts: Synthetic strategies, performance boost, and application advances DOI

Tairan Pang,

Guanhua Wang,

Wenjie Sui

и другие.

Coordination Chemistry Reviews, Год журнала: 2025, Номер 528, С. 216426 - 216426

Опубликована: Янв. 10, 2025

Язык: Английский

Процитировано

4
Plant secondary metabolites against biotic stresses for sustainable crop protection DOI
Tanzim Jahan, Md. Nurul Huda, Kaixuan Zhang

и другие.

Biotechnology Advances, Год журнала: 2025, Номер unknown, С. 108520 - 108520

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

2
Glycine betaine and MiWRKY53 enhance antioxidant capacity and disease resistance against Colletotrichum gloeosporioides in mango fruit DOI
X. L. Niu, Yuxin Hu, Xin Wang

и другие.

Postharvest Biology and Technology, Год журнала: 2025, Номер 224, С. 113464 - 113464

Опубликована: Фев. 21, 2025

Язык: Английский

Процитировано

0
Ursolic Acid Induces Multifaceted Defense Responses Against Postharvest Blue Mold Rot in Apple Fruit DOI Creative Commons
Chang Shu, Wenxiao Jiao, Kuanbo Cui

и другие.

Foods, Год журнала: 2025, Номер 14(5), С. 761 - 761

Опубликована: Фев. 23, 2025

The disease resistance and defense mechanisms induced by ursolic acid (UA) in apple fruit were studied this paper. UA was directly mixed with potato dextrose agar broth media to assay its antifungal activity vitro. results showed that exerted inherent inhibited the vitro growth spore germination of Penicillium expansum. Its half-maximal inhibitory concentration for hyphal 175.6 mg L-1. Apple immersed solution, followed inoculation P. expansum, measure their response. demonstrated significant are multifaceted associated defensive antioxidative enzymes phenylpropanoid pathway. Chitinase, β-1,3-glucanase, peroxidase, polyphenol oxidase activated maintained at relatively high levels. activities metabolites pathway, including phenylalanine ammonia-lyase, cinnamate-4-hydroxylase, 4-coumarate coenzyme A ligase significantly increased; accordingly, total phenolics, flavonoid, lignin contents increased. superoxide dismutase, ascorbate glutathione reductase enhanced upon treatment, while catalase suppressed, which regulates hydrogen peroxide accumulation defend against pathogens. These suggest induces responses postharvest blue mold rot it may be a promising elicitor induce control decay.

Язык: Английский

Процитировано

0
Valorization of lignin as an active agent in sustainable agriculture: A review DOI
Pati Kemala, Arief Heru Prianto, Antonio Di Martino

и другие.

Journal of Industrial and Engineering Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
A dual transcriptome analysis reveals accession-specific resistance responses in Lathyrus sativus against Erysiphe pisi DOI Creative Commons

Rita M. Maravilha,

Telma Fernandes, Pedro M. Barros

и другие.

Frontiers in Plant Science, Год журнала: 2025, Номер 16

Опубликована: Март 5, 2025

Lathyrus sativus (grass pea) is a valuable crop for sustainable agriculture, offering dietary benefits and desirable agronomic traits. However, its yield stability limited by diseases such as powdery mildew caused Erysiphe pisi. Increasing fungal resistance to pesticides environmental concerns demand the development of resistant varieties. To identify key defense mechanisms effector genes involved in sativus-Erysiphe pisi interaction we analyzed four L. accessions exhibiting varying E. (resistant, partially resistant, susceptible, susceptible) using dual RNA-Seq experiment across different time points. We observed host biphasic response, characterized an initial burst gene expression, followed quiescent phase, subsequent wave intense expression. Common included antifungal protein cell wall reinforcement, reactive oxygen species-mediated defense. These defenses respectively Bowman-Birk type proteinase inhibitors, peptidyl-prolyl cis-trans isomerases mannitol dehydrogenases. The accession specifically activated early reinforcement structural barriers associated with lignin biosynthesis phenylpropanoid pathway, along sustained chemical (e.g. eugenol synthase 1), epigenetic regulation, oxidative stress responses thorough peroxidases heat shock proteins. partial exhibited front-loaded response at infection stages. Contrastingly, susceptible weaker baseline defense, slower less robust targeting pathogen infection. identified potential effectors, including hydrolysis mannosidase DCW1), nutrient acquisition secreted alpha-glucosidase), virulence SnodProt1), higher diversity effectors accession. In conclusion, this study identifies novel targets NLRs proteins related within complex support future breeding programs aimed enhancing species.

Язык: Английский

Процитировано

0
CRISPR/dCas12a-mediated activation of SlPAL2 enhances tomato resistance against bacterial canker disease DOI Creative Commons
Diana Marcela Rivera-Toro, Stefan de Folter, Raúl Álvarez-Venegas

и другие.

PLoS ONE, Год журнала: 2025, Номер 20(3), С. e0320436 - e0320436

Опубликована: Март 26, 2025

Crop protection is essential for maintaining and improving agricultural productivity. While pesticides are commonly used to control pests, they pose several challenges, including environmental harm health risks. Alternative strategies include breeding resistant crop varieties, biological control, utilizing genome-editing tools like CRISPR/Cas. However, the application of epigenome editing, particularly CRISPR activation (CRISPRa), in plants remains underexplored. Phenylalanine ammonia-lyase (PAL), a key enzyme phenylpropanoid pathway, plays pivotal role plant defense by producing lignin other secondary metabolites pathogen resistance. In this study, we engineered tomato fusing SET-domain SlATX1 coding gene, histone H3 lysine 4 tri-methyltransferase, dCas12a, targeting SlPAL2 promoter with aim increase PAL2 gene expression. CRISPRa-edited demonstrated increased deposition H3K4me3 epigenetic mark significantly upregulated This enhanced accumulation conferred resistance Clavibacter michiganensis subsp. (Cmm) without significant reduction height or fruit yield. Disease was also associated reduced load lesion size, higher levels persisted even after expression declined post-infection. These findings highlight potential CRISPRa reprogramming responses through targeted modifications, offering sustainable approach improvement. Furthermore, could be applied enhance resilience contexts, such as addressing food security challenges enhancing

Язык: Английский

Процитировано

0
Plant Secondary Metabolites—Central Regulators Against Abiotic and Biotic Stresses DOI Creative Commons
Ameer Khan,

Farah Kanwal,

Sana Ullah

и другие.

Metabolites, Год журнала: 2025, Номер 15(4), С. 276 - 276

Опубликована: Апрель 16, 2025

As global climates shift, plants are increasingly exposed to biotic and abiotic stresses that adversely affect their growth development, ultimately reducing agricultural productivity. To counter these stresses, produce secondary metabolites (SMs), which critical biochemical essential compounds serve as primary defense mechanisms. These diverse compounds, such alkaloids, flavonoids, phenolic nitrogen/sulfur-containing act natural protectants against herbivores, pathogens, oxidative stress. Despite the well-documented protective roles of SMs, precise mechanisms by environmental factors modulate accumulation under different stress conditions not fully understood. This review provides comprehensive insights into recent advances in understanding functions SMs plant emphasizing regulatory networks biosynthetic pathways. Furthermore, we explored unique contributions individual SM classes responses while integrating findings across entire spectrum diversity, providing a resilience multiple conditions. Finally, highlight emerging strategies for harnessing improve crop through genetic engineering present novel solutions enhance sustainability changing climate.

Язык: Английский

Процитировано

0
Lignin: Structural Advantages DOI
Ajay Kumar

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
Transgenic poplar for resistance against pest and pathogen attack in forests: an overview DOI Creative Commons

S. Sharan,

Amrita Chakraborty, Amit Roy

и другие.

Frontiers in Forests and Global Change, Год журнала: 2024, Номер 7

Опубликована: Дек. 18, 2024

Forests are potential habitats for immense terrestrial ecosystems and aquatic biodiversity, performing an essential role in ecological preservation regulation of climate. The anthropogenic pressures on the forests lead to forest loss, fragmentation degradation. Requirements sustainable methodologies protection utmost priority under climate change regime. Among trees, poplar trees ( Populus L.) have attracted attention global forestry as a promising material improving quality quantity urban landscapes. These plants provide wood, which can be utilized raw resources paper industry source biofuel. However, several biotic stresses, such attacks by pests pathogens, severely affect production productivity. improvement through conventional tree breeding methods is restricted due their long-life cycles lack suitable donors with resistance genes. has been model plant studying gene functions its highly efficient genetic transformation capabilities. present review will comprehensive overview pest pathogen poplar, focusing infection mechanisms, transmission routes, control strategies. Additionally, it examine most widely used (gene gun-mediated, Agrobacterium tumefaciens -mediated, protoplast transformation, micro-RNA mediated clustered regularly interspaced short palindromic repeats (CRISPR)-associated (CRISPR-Cas) systems RNA interference) tolerance against pathogens attack. Furthermore, delve into prospects, challenges, recent advances molecular biology tools safe application improve insect trees. Finally, regeneration transgenic enhanced resistance, developed various engineering techniques, discussed.

Язык: Английский

Процитировано

2