Gut microbiota and blood biomarkers in IBD-Related arthritis: insights from mendelian randomization

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Янв. 2, 2025

With the ongoing rise in incidence of inflammatory bowel disease (IBD), its extraintestinal manifestations have garnered significant attention. IBD-related arthritis is notable for insidious onset and unpredictability, presenting considerable challenges clinical diagnosis management. Factors such as gut microbiota, plasma proteins, biomarkers found blood urine may be closely associated with arthritis. However, mechanisms by which these factors influence this condition remain poorly understood require urgent investigation. We employed method linkage disequilibrium two-sample Mendelian randomization (MR) approach, utilizing single nucleotide polymorphisms (SNPs) identified from large-scale genome-wide association studies instrumental variables. In scientifically rigorous manner, we explored potential causal relationship between relation to resulting (IBD). This aids elucidating roles development following IBD, while minimizing confounding reverse causality commonly encountered observational studies. To further verify strengthen our findings, conducted subsequent sensitivity analyses. These analyses will evaluate strength SNPs studied biomarkers, well post-IBD arthritis, accounting variations SNP distribution among populations other genetic influencing factors. Through analytical steps, objective enhance robustness credibility research findings provide more reliable scientific evidence regarding pathogenesis MR analysis provides genetically predicted risk investigates characteristics associations specific Among pterin-4-alpha-carbinolamine dehydratase, aldo-keto reductase family 1 member C4, cathepsin L2, angiostatin, hepatocyte growth factor-like protein, hepatitis A virus cellular receptor 2, protein O-linked mannose beta-1,4-N-acetylglucosaminyltransferase epididymal-specific alpha-mannosidase, platelet-derived factor receptor-like are Crohn's disease-related contrast, agrin, methylenetetrahydrofolate synthetase domain-containing neurotrophin-3 (NT-3) receptor, neuropilin-1 ulcerative colitis-related Furthermore, bacterial pathway abundance, adenosylcobalamin, N-acetylglucosamine, N-acetylmannosamine, N-acetylneuraminic acid degradation, glycolysis metabolism degradation pathways, Meanwhile, abundance (pentose phosphate pathway) microbiota (Bacteroidetes, Bacteroidia, Bacteroidales, Porphyromonadaceae, Faecalibacterium, Eubacterium eligens) linked Notably, did not identify any connections factors, Lastly, study, insufficient number available precluded detection a relationship. study employs elucidate relationships occurrence progression offers novel perspective deeper understanding highlights future directions treatment strategies condition.

Язык: Английский

---

Protect Effects of Perilla Seed Extract and Its Active Ingredient Luteolin Against Inflammatory Bowel Disease Model via the PI3K/AKT Signal Pathway In Vivo and In Vitro

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(8), С. 3564 - 3564

Опубликована: Апрель 10, 2025

The purpose of this study was to investigate the anti-inflammatory effects Perilla Seed Extract (PSE) and its active ingredient on Inflammatory Bowel Disease (IBD) in vitro vivo. Thirty-two C57/BL Mice were randomly divided into four groups (n = 8): control group (CON), PBS group, LPS (LPS 3.5 mg/kg given intraperitoneally [ip] day 7 only), PSE (100 orally daily + ip at 7). euthanized 24 h after administration. MODE-K cells five groups: (50 μg/mL for 2 h), (low dose, 25 LPS; middle 50 high 100 LPS). In vivo, compared with CON revealed a significant decrease villus length-to-crypt depth ratio (p < 0.01) goblet cell density per unit area 0.01). Conversely, administration resulted increase significantly increased ROS content 0.01), secretion inflammatory cytokines IL-6 TNF-α mRNA expressions HO-1 decreased Occludin Claudin1 contrast, treatment led marked levels along reduction factors TNF-α(p 0.05), as well Concurrently, 0.05) vitro, also reversed LPS-induced inflammation, oxidation tight junction–related factors. Network pharmacology identified 97 potential targets treating IBD, while transcriptomics analysis 342 differentially expressed genes (DEGs). indicated that pathways included PI3K-Akt signaling pathway, MAPK TNF which PI3K-AKT pathway may represent primary mechanism. an vivo setting, protein expression p-PI3K/PI3K p-AKT1/AKT1 blocked chemical composition analyzed using UPLC-MS/MS, six components including luteolin (content 0.41%), rosmarinic acid 0.27%), α-linolenic 1.2%), oleic 0.2%). Molecular docking found could establish stable binding eight targets, cells. summary, demonstrates efficacy against IBD progression by enhancing intestinal barrier function inhibiting responses oxidative stress via PI3K/AKT luteolin’s inhibition AKT1 phosphorylation appears play particularly crucial role therapeutic

Язык: Английский

---

RDA-direct targeted flavonoids profiling strategy combined with fingerprinting and chemometrics for quality evaluation of Changshan Huyou flower

Microchemical Journal, Год журнала: 2025, Номер unknown, С. 113204 - 113204

Опубликована: Фев. 1, 2025

Язык: Английский

---

The physicochemical properties of low molecular weight ‘Chachi’ (Citrus reticulata ‘Chachi’) pectins prepared by different modification methods and their modulation effects on the human gut microbiota

Food Hydrocolloids, Год журнала: 2025, Номер unknown, С. 111350 - 111350

Опубликована: Март 1, 2025

Язык: Английский

---

An ultrasonic degraded polysaccharide extracted from Pueraria lobata ameliorate ischemic brain injury in mice by regulating the gut microbiota and LPS-TLR4 pathway

Ultrasonics Sonochemistry, Год журнала: 2024, Номер 112, С. 107200 - 107200

Опубликована: Дек. 13, 2024

Язык: Английский

---

Oral Administration of Bioactive Nanoparticulates for Inflammatory Bowel Disease Therapy by Mitigating Oxidative Stress and Restoring Intestinal Microbiota Homeostasis

Molecular Pharmaceutics, Год журнала: 2024, Номер unknown

Опубликована: Окт. 27, 2024

The management of inflammatory bowel disease (IBD) continues to pose significant challenges due the absence curative therapies and a high rate recurrence. Therefore, it is imperative explore novel approaches enhance efficacy IBD therapy. Herein, bioactive nanoparticulate s tailored designed achieve "Pull-Push" approach for efficient safe treatment by integrating reactive oxygen species (ROS) scavenging (Pull) with anti-inflammatory agent delivery (Push) in microenvironment. multifunctional nanomedicine, designated MON-PAMAM@SASP, developed through encapsulation sulfasalazine (SASP), widely utilized clinical drug IBD, within cationic diselenide-bridged mesoporous organosilica nanoparticles (MONs) that possess antioxidant properties. poly(amidoamine) (PAMAM) endows original MONs positive charge characteristics. MON-PAMAM@SASP not only displays remarkable capability neutralizing ROS ameliorates intestinal damage, but also achieves controllable release SASP mitigate inflammation. Consequently, this nanomedicine effectively mitigates colitis mouse models, our current research has identified any toxicity. Beyond regulating microenvironment intestine, results increased richness restores microbiota homeostasis, thereby mitigating certain extent. Together, work provides highly versatile encourages development similar treating multiple diseases gastrointestinal tract.

Язык: Английский

---

结直肠癌肠道微生物群的性二态性

Chinese Science Bulletin (Chinese Version), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 1, 2024

---