Natural Product Research,
Год журнала:
2024,
Номер
unknown, С. 1 - 11
Опубликована: Дек. 29, 2024
Sarcosphaera
coronaria,
a
poisonous
mushroom
safe
for
consumption
when
cooked
properly,
was
investigated
cytotoxic
metabolites
against
HeLa
(cervical),
HT-29
(adenocarcinoma),
and
MCF-7
(breast)
cancer
cell
lines.
Polarity-based
extracts
from
raw
baked
parts
were
screened,
advancing
acetone
methanol
detailed
mycochemical
analysis.
Four
new
compounds
isolated
the
extract,
including
three
amides:
N-dodecanoyl-N-(ethylsulfinyl)
hexadecanamide
(1),
N-(1,3,4-trihydroxyoctadecan-2-yl)
tetracosanamide
(2),
N-(1,3,4-trihydroxycosane-2-yl)
(3)
1,2-dilinolenoyl
glycerol
(4).
Compounds
1
2
obtained
mushroom,
while
3
4
mushroom.
Structural
elucidation
achieved
via
spectroscopic
techniques,
IR,
1D
2D
NMR,
MS.
Compound
exhibited
significant
activity
lines
with
IC50
values
of
52.44
±
2.1
71.51
0.8
µg/mL,
respectively,
showed
moderate
HT29
line
(IC50:
86.85
1.7
µg/mL).
similar
83.83
1.5
84.27
3.2
µg/mL),
MCF7
85.91
86.41
2.7
90.91
3.3
93.74
1.48
µg/mL
µg/mL)
lines,
respectively.
Due
to
presence
these
bioactive
components,
has
potential
be
developed
into
valuable
source
nutraceuticals.
Fermentation,
Год журнала:
2025,
Номер
11(2), С. 96 - 96
Опубликована: Фев. 13, 2025
Hyperuricemia
(HUA),
characterized
by
elevated
serum
uric
acid
(UA)
levels,
is
a
key
risk
factor
for
gout.
In
human
purine
metabolism,
approximately
70%
of
UA
excreted
via
the
kidneys,
while
remaining
30%
eliminated
through
intestines.
Thus,
intestinal
microbiota
plays
crucial
role
in
regulating
metabolism
gut–kidney
axis.
However,
detailed
mechanisms
which
reduces
levels
and
supports
kidney
health
remain
unclear.
this
study,
researchers
investigated
potential
Lacticaseibacillus
paracasei
LT12,
strain
exhibiting
xanthine
oxidase
(XO)
inhibition
activity
ability
to
degrade
inosine
guanosine,
reducing
hyperuricemia
mouse
model.
was
induced
gavaging
mice
with
300
mg/kg
potassium
oxonate
hypoxanthine
two
weeks.
The
subsequent
4-week
intervention
included
five
groups:
normal
control
group,
model
positive
group
receiving
allopurinol
(5
body
weight),
low-dose
LT12
(1.5
×
10⁶
CFU/kg),
high-dose
(4.5
10⁹
CFU/kg).
results
demonstrated
that
L.
effectively
reduced
inhibited
hepatic
XO
activity,
regulated
renal
transporter
proteins
(OAT1,
URAT1,
GLUT9,
ABCG2),
abundance
pathogenic
bacterium
Corynebacterium
stationis
both
groups.
Notably,
only
significantly
increased
gut
butyrate
levels.
conclusion,
shows
promise
as
probiotic
ameliorating
hyperuricemia.
Future
clinical
studies
are
needed
validate
its
efficacy.
Natural Product Communications,
Год журнала:
2025,
Номер
20(3)
Опубликована: Март 1, 2025
Background
The
leaves
and
rhizomes
of
Melicope
pteleifolia
are
utilized
for
their
therapeutic
properties,
being
applied
to
treat
various
ailments
such
as
scabies,
wounds,
ulcers,
sore
throats,
rheumatism,
eczema,
dermatitis,
bruises,
snake
bites.
goal
this
work
is
investigate
the
anti-inflammatory
xanthine
oxidase
inhibitory
activities
well
isolation
bioactive
compounds
extracts
fractions
from
ptelefolia
.
Methods
dried
plants
were
extracted
exhaustedly
with
96%
ethanol,
solvent
was
evaporated,
resulting
(MPE)
partitioned
successively
hexane,
ethyl
acetate,
methanol
through
flash
chromatography
obtain
three
sub-fractions
(MPH,
MPEA,
MPM).
macrophage
cell
line
RAW264.7
used
assess
activity
via
inhibition
secretion
mediator
(NO
TNF-α).
DPPH,
ABTS,
RP
assay,
antioxidant
reducing
uric
acid
in
vitro,
respectively.
Structural
chemicals
identified
by
NMR
methods.
Results
All
manifested
activities.
MPEA
fraction
showed
a
better
NO
effects
than
that
MPE
MPH,
while
MPH
stronger
those
MPE,
MPM
TNF-α
experiment.
From
most
active
(MPEA),
eight
(1-8)
isolated
column
chromatography.
Of
which,
quercetin
(3),
quercitrin
(7),
kaempferol
(2),
Luteolin
7
glucoside
(8)
potential
anti-inflammation
suppressing
oxidase.
Conclusions
exhibited
significant
anti-inflammatory,
antioxidant,
showing
it
promising
source
developing
medications
targeting
inflammation
elevated
levels.
Its
potentially
pharmaceuticals
conditions
like
gout
other
inflammatory
illnesses.
Journal of Chemical Information and Modeling,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 24, 2025
Bioactive
peptides
from
food
sources
offer
a
safe
and
biocompatible
approach
to
enzyme
inhibition,
with
potential
applications
in
managing
metabolic
disorders
such
as
hyperuricemia
gout,
conditions
linked
excessive
xanthine
oxidase
activity.
Using
machine
learning-based
screening
inspired
by
the
bioactivity
of
natto,
two
peptides,
ECFK
FECK,
were
identified
Bacillus
subtilis
proteome
validated
inhibitors
IC50
values
37.36
71.57
mM,
respectively.
Further
experiments
confirmed
their
safety
through
cytotoxicity
assays,
electronic
tongue
analysis
demonstrated
mild
sensory
properties,
supporting
edibility.
Molecular
dynamics
simulations
revealed
that
these
stabilize
critical
regions,
showing
higher
dissociation
energy
barrier
(52.08
kcal/mol)
than
FECK
(46.39
kcal/mol),
indicating
strong,
stable
interactions.
This
study
highlights
food-derived
natural
oxidase,
offering
promising
therapeutic
for
disorder
management.
Antioxidants,
Год журнала:
2024,
Номер
14(1), С. 38 - 38
Опубликована: Дек. 31, 2024
Oxidative
stress
plays
a
pivotal
role
in
the
pathogenesis
and
progression
of
cardiovascular
diseases
(CVDs).
This
review
focuses
on
signaling
pathways
oxidative
during
development
CVDs,
delving
into
molecular
regulatory
networks
underlying
various
disease
stages,
particularly
apoptosis,
inflammation,
fibrosis,
metabolic
imbalance.
By
examining
dual
roles
influences
sex
differences
levels
susceptibility,
this
study
offers
comprehensive
understanding
diseases.
The
integrates
key
findings
from
current
research
three
ways.
First,
it
outlines
major
CVDs
associated
with
their
respective
pathways,
emphasizing
stress’s
central
pathology.
Second,
summarizes
protective
effects,
mechanisms
action,
animal
models
antioxidants,
offering
insights
future
drug
development.
Third,
discusses
applications,
advantages,
limitations,
potential
targets
gene
therapy
providing
foundation
for
novel
therapeutic
strategies.
These
tables
underscore
systematic
integrative
nature
while
theoretical
basis
precision
treatment
CVDs.
A
contribution
is
differential
effects
across
different
stages
addition
to
proposal
innovative,
multi-level
intervention
strategies,
which
open
new
avenues
system.
