Foods, Год журнала: 2024, Номер 13(24), С. 4150 - 4150

Опубликована: Дек. 21, 2024

This study optimized ultrasound-assisted enzymatic (UAE) extraction of TMP (Tricholoma matsutake polysaccharide) through response surface methodology. The optimal conditions included complex enzyme comprising 1.15% cellulase, 0.60% pectinase, and 0.95% dispase, with ultrasound for 24 min at 84.5 °C hydrolysis pH 5.0. process yielded 19.74 ± 0.51% TMP, exceeding traditional hot water by over four times. Fourier transform infrared spectroscopy (FT–IR) confirmed that UAE did not alter the structure TMP. In vitro experiments indicated TMP-UAE demonstrated enhanced antioxidant properties. Further purification DEAE-52 Sephadex G-100 chromatography resulted in a homogenous polysaccharide fraction (TMP). Characterization has an average molecular weight 2.79 × 104 Da, composed fucose, galactose, glucose mannose 2.00:9.44:86.29:2.28 molar ratio. FT–IR presence C-O-C glycosidic bonds pyranyl-type sugar rings. Scanning electron microscopy displayed loose lamellar structures small pores. Finally, exhibited therapeutic potential against C. elegans Parkinson’s disease, including reducing α-synuclein aggregation, protecting dopaminergic neurons, prolonging lifespan. provides efficient method insight into its neuroprotective effect PD C.elegans.

Язык: Английский