Ultrasound-Assisted Enzymatic Extraction of Polysaccharides from Tricholoma matsutake: Optimization, Structural Characterization, and Inhibition of α-Synuclein Aggregation DOI Creative Commons
Wen Gao, Yang Wang, Fuping Lu

et al.

Foods, Journal Year: 2024, Volume and Issue: 13(24), P. 4150 - 4150

Published: Dec. 21, 2024

This study optimized ultrasound-assisted enzymatic (UAE) extraction of TMP (Tricholoma matsutake polysaccharide) through response surface methodology. The optimal conditions included complex enzyme comprising 1.15% cellulase, 0.60% pectinase, and 0.95% dispase, with ultrasound for 24 min at 84.5 °C hydrolysis pH 5.0. process yielded 19.74 ± 0.51% TMP, exceeding traditional hot water by over four times. Fourier transform infrared spectroscopy (FT–IR) confirmed that UAE did not alter the structure TMP. In vitro experiments indicated TMP-UAE demonstrated enhanced antioxidant properties. Further purification DEAE-52 Sephadex G-100 chromatography resulted in a homogenous polysaccharide fraction (TMP). Characterization has an average molecular weight 2.79 × 104 Da, composed fucose, galactose, glucose mannose 2.00:9.44:86.29:2.28 molar ratio. FT–IR presence C-O-C glycosidic bonds pyranyl-type sugar rings. Scanning electron microscopy displayed loose lamellar structures small pores. Finally, exhibited therapeutic potential against C. elegans Parkinson’s disease, including reducing α-synuclein aggregation, protecting dopaminergic neurons, prolonging lifespan. provides efficient method insight into its neuroprotective effect PD C.elegans.

Language: Английский

Electrospun microfibers of zein and polyethylene oxide: A study on pequi carotenoid encapsulation DOI
Caroline Ramos‐Souza, Letícia G. da Trindade, Anna Rafaela Cavalcante Braga

et al.

Food Hydrocolloids, Journal Year: 2025, Volume and Issue: 166, P. 111299 - 111299

Published: March 1, 2025

Language: Английский

Citations

0
Structural Characterization and Bioactivity Evaluation of Polysaccharide from Ziziphus Jujuba Mill DOI
Ziyi Zhang, Miaomiao Tian, Kaixuan Li

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Characterization of two pectic polysaccharides from Rosa roxburghii Tratt fruit and their protection on Bifidobacterium against antibiotic damage DOI
Tingting Tu, Xiaohui Yuan, Yu-Heng Mao

et al.

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 106340 - 106340

Published: March 1, 2025

Language: Английский

Citations

0
Monosaccharide composition and glycosidic linkages analysis by ultra high performance liquid chromatography-triple quadrupole tandem mass spectrometry—Case study of plant polysaccharides DOI
Yanyun Zhu,

Yunyun Zheng,

Bingge Liu

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 281, P. 136471 - 136471

Published: Oct. 9, 2024

Language: Английский

Citations

2
Optimizing solvothermal synthesis of aluminum-loaded pomelo peel for enhanced fluoride adsorption using response surface methodology DOI

Junjie Gu,

Ying Miao,

Dezhen Zhu

et al.

Industrial Crops and Products, Journal Year: 2024, Volume and Issue: 222, P. 119932 - 119932

Published: Nov. 1, 2024

Language: Английский

Citations

2
Preparation methods, structural features, biological activities and potential applications of Ophiopogon japonicus polysaccharides: An updated review DOI
Tongtong Zhu, Yi Wang, Ju Liu

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 290, P. 139059 - 139059

Published: Dec. 20, 2024

Language: Английский

Citations

1
Ultrasound-Assisted Enzymatic Extraction of Polysaccharides from Tricholoma matsutake: Optimization, Structural Characterization, and Inhibition of α-Synuclein Aggregation DOI Creative Commons
Wen Gao, Yang Wang, Fuping Lu

et al.

Foods, Journal Year: 2024, Volume and Issue: 13(24), P. 4150 - 4150

Published: Dec. 21, 2024

This study optimized ultrasound-assisted enzymatic (UAE) extraction of TMP (Tricholoma matsutake polysaccharide) through response surface methodology. The optimal conditions included complex enzyme comprising 1.15% cellulase, 0.60% pectinase, and 0.95% dispase, with ultrasound for 24 min at 84.5 °C hydrolysis pH 5.0. process yielded 19.74 ± 0.51% TMP, exceeding traditional hot water by over four times. Fourier transform infrared spectroscopy (FT–IR) confirmed that UAE did not alter the structure TMP. In vitro experiments indicated TMP-UAE demonstrated enhanced antioxidant properties. Further purification DEAE-52 Sephadex G-100 chromatography resulted in a homogenous polysaccharide fraction (TMP). Characterization has an average molecular weight 2.79 × 104 Da, composed fucose, galactose, glucose mannose 2.00:9.44:86.29:2.28 molar ratio. FT–IR presence C-O-C glycosidic bonds pyranyl-type sugar rings. Scanning electron microscopy displayed loose lamellar structures small pores. Finally, exhibited therapeutic potential against C. elegans Parkinson’s disease, including reducing α-synuclein aggregation, protecting dopaminergic neurons, prolonging lifespan. provides efficient method insight into its neuroprotective effect PD C.elegans.

Language: Английский

Citations

1