Comparative Analysis of phaC1 and phaC2 Genes Encoding Polyhydroxyalkanoate Synthase in Cupriavidus malaysiensis USMAA1020T DOI Open Access

Nur Asilla Hani Shafie,

Nor Azura Azami, Nyok‐Sean Lau

и другие.

Journal of Basic Microbiology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 18, 2025

ABSTRACT Two polyhydroxyalkanoate synthase genes, phaC1 and phaC2 , were identified in three strains of Cupriavidus malaysiensis ( C. ): USMAA1020 T USMAHM13, USMAA2‐4. Interestingly, the genome revealed presence granule‐associated protein phaF ), which was not present USMAHM13 A Maximum Likelihood phylogenetic analysis shows that phaC genes classified into Class I synthases. The formed a separate, distinct cluster. To further examine function both cloned from individually expressed necator ) PHB‐4, serves as benchmark functionality for other strains. Using γ‐butyrolactone sole carbon source, poly(3‐hydroxybutyrate‐ co ‐4‐hydroxybutyrate) contains up to 83.00 mol% 4‐hydroxybutyrate (4HB) 26.50% PHA content. However, transformant PHB‐4 with produced only 2.30% content no 4HB monomer. produces 100 3HB monomer 41.90% content, while 25.80% when using oleic acid source. When provided mixed substrate 1‐pentanol, transconjugants accumulated 20% but low 3HV 4%–5%. These findings significantly contribute scientific literature by improving understanding genetic biochemical diversity two synthases, species.

Язык: Английский

Comparative Analysis of phaC1 and phaC2 Genes Encoding Polyhydroxyalkanoate Synthase in Cupriavidus malaysiensis USMAA1020T DOI Open Access

Nur Asilla Hani Shafie,

Nor Azura Azami, Nyok‐Sean Lau

и другие.

Journal of Basic Microbiology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 18, 2025

ABSTRACT Two polyhydroxyalkanoate synthase genes, phaC1 and phaC2 , were identified in three strains of Cupriavidus malaysiensis ( C. ): USMAA1020 T USMAHM13, USMAA2‐4. Interestingly, the genome revealed presence granule‐associated protein phaF ), which was not present USMAHM13 A Maximum Likelihood phylogenetic analysis shows that phaC genes classified into Class I synthases. The formed a separate, distinct cluster. To further examine function both cloned from individually expressed necator ) PHB‐4, serves as benchmark functionality for other strains. Using γ‐butyrolactone sole carbon source, poly(3‐hydroxybutyrate‐ co ‐4‐hydroxybutyrate) contains up to 83.00 mol% 4‐hydroxybutyrate (4HB) 26.50% PHA content. However, transformant PHB‐4 with produced only 2.30% content no 4HB monomer. produces 100 3HB monomer 41.90% content, while 25.80% when using oleic acid source. When provided mixed substrate 1‐pentanol, transconjugants accumulated 20% but low 3HV 4%–5%. These findings significantly contribute scientific literature by improving understanding genetic biochemical diversity two synthases, species.

Язык: Английский

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