Comparative Analysis of phaC1 and phaC2 Genes Encoding Polyhydroxyalkanoate Synthase in Cupriavidus malaysiensis USMAA1020T DOI Open Access

Nur Asilla Hani Shafie,

Nor Azura Azami, Nyok‐Sean Lau

et al.

Journal of Basic Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 18, 2025

ABSTRACT Two polyhydroxyalkanoate synthase genes, phaC1 and phaC2 , were identified in three strains of Cupriavidus malaysiensis ( C. ): USMAA1020 T USMAHM13, USMAA2‐4. Interestingly, the genome revealed presence granule‐associated protein phaF ), which was not present USMAHM13 A Maximum Likelihood phylogenetic analysis shows that phaC genes classified into Class I synthases. The formed a separate, distinct cluster. To further examine function both cloned from individually expressed necator ) PHB‐4, serves as benchmark functionality for other strains. Using γ‐butyrolactone sole carbon source, poly(3‐hydroxybutyrate‐ co ‐4‐hydroxybutyrate) contains up to 83.00 mol% 4‐hydroxybutyrate (4HB) 26.50% PHA content. However, transformant PHB‐4 with produced only 2.30% content no 4HB monomer. produces 100 3HB monomer 41.90% content, while 25.80% when using oleic acid source. When provided mixed substrate 1‐pentanol, transconjugants accumulated 20% but low 3HV 4%–5%. These findings significantly contribute scientific literature by improving understanding genetic biochemical diversity two synthases, species.

Language: Английский

Comparative Analysis of phaC1 and phaC2 Genes Encoding Polyhydroxyalkanoate Synthase in Cupriavidus malaysiensis USMAA1020T DOI Open Access

Nur Asilla Hani Shafie,

Nor Azura Azami, Nyok‐Sean Lau

et al.

Journal of Basic Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 18, 2025

ABSTRACT Two polyhydroxyalkanoate synthase genes, phaC1 and phaC2 , were identified in three strains of Cupriavidus malaysiensis ( C. ): USMAA1020 T USMAHM13, USMAA2‐4. Interestingly, the genome revealed presence granule‐associated protein phaF ), which was not present USMAHM13 A Maximum Likelihood phylogenetic analysis shows that phaC genes classified into Class I synthases. The formed a separate, distinct cluster. To further examine function both cloned from individually expressed necator ) PHB‐4, serves as benchmark functionality for other strains. Using γ‐butyrolactone sole carbon source, poly(3‐hydroxybutyrate‐ co ‐4‐hydroxybutyrate) contains up to 83.00 mol% 4‐hydroxybutyrate (4HB) 26.50% PHA content. However, transformant PHB‐4 with produced only 2.30% content no 4HB monomer. produces 100 3HB monomer 41.90% content, while 25.80% when using oleic acid source. When provided mixed substrate 1‐pentanol, transconjugants accumulated 20% but low 3HV 4%–5%. These findings significantly contribute scientific literature by improving understanding genetic biochemical diversity two synthases, species.

Language: Английский

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