Journal of Pharmaceutical Analysis,
Год журнала:
2024,
Номер
15(3), С. 101141 - 101141
Опубликована: Ноя. 8, 2024
Mitochondria
play
a
crucial
role
as
organelles,
managing
several
physiological
processes
such
redox
balance,
cell
metabolism,
and
energy
synthesis.
Initially,
the
assumption
was
that
mitochondria
primarily
resided
in
host
cells
could
exclusively
transmit
from
oocytes
to
offspring
by
mechanism
known
vertical
inheritance
of
mitochondria.
Recent
scholarly
works,
however,
suggest
certain
types
their
other
developmental
via
referred
intercellular
or
horizontal
mitochondrial
transfer.
This
review
details
process
which
are
transferred
across
explains
impact
transfer
between
on
efficacy
functionality
cancer
various
forms.
Specifically,
we
regulating
cellular
metabolism
restoration,
excess
reactive
oxygen
species
(ROS)
generation,
proliferation,
invasion,
metastasis,
mitophagy
activation,
DNA
(mtDNA)
inheritance,
immune
system
modulation
therapeutic
resistance
cancer.
Additionally,
highlight
possibility
using
approach
treat
enhance
treatments.
Abstract
Mitochondria
are
vital
organelles
whose
impairment
leads
to
numerous
metabolic
disorders.
Mitochondrial
transplantation
serves
as
a
promising
clinical
therapy.
However,
its
widespread
application
is
hindered
by
the
limited
availability
of
healthy
mitochondria,
with
dose
required
reaching
up
10
9
mitochondria
per
injection/patient.
This
necessitates
sustainable
and
tractable
approaches
for
producing
high-quality
human
mitochondria.
In
this
study,
we
demonstrated
highly
efficient
mitochondria-producing
strategy
manipulating
mitobiogenesis
tuning
organelle
balance
in
mesenchymal
stem
cells
(MSCs).
Utilizing
an
optimized
culture
medium
(mito-condition)
developed
from
our
established
formula,
achieved
854-fold
increase
production
compared
normal
MSC
within
15
days.
These
were
not
only
significantly
expanded
but
also
exhibited
superior
function
both
before
after
isolation,
ATP
levels
5.71
times
that
Mechanistically,
revealed
activation
AMPK
pathway
establishment
novel
cellular
state
ideal
mitochondrial
fabrication,
characterized
enhanced
proliferation
while
suppressing
other
energy-consuming
activities.
Furthermore,
vivo
these
was
validated
mitotherapy
mouse
osteoarthritis
model,
resulting
significant
cartilage
regeneration
over
12-week
period.
Overall,
study
presented
new
off-the-shelf
fabrication
provided
insights
into
molecular
mechanisms
governing
synthesis.
Research Square (Research Square),
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 25, 2025
Abstract
Traumatic
brain
injury
(TBI)
remains
a
significant
clinical
challenge
due
to
the
absence
of
effective
therapies
for
mitigating
oxidative
damage
and
neuronal
dysfunction.
Given
brain’s
high
metabolic
demand,
mitochondrial
transplantation
has
emerged
as
promising
therapeutic
strategy.
However,
identifying
an
optimal,
functional
source
mitochondria
major
obstacle.
In
this
study,
we
explore
potential
isolated
from
senescent
platelets
readily
available,
GMP-compliant
promoting
recovery
in
TBI
models.
was
induced
rats
using
weight-drop
model,
with
confirmation
through
histopathological
analysis.
The
animals
were
then
divided
into
four
experimental
groups:
healthy
control,
TBI,
+
RB
(receiving
respiration
buffer),
Mito
transplantation).
Neurobehavioural
assessed
series
sensorimotor
tests,
including
beam
walk
test,
horizontal
bars
grid-walking
cylinder
test.
Our
results
show
that
significantly
improved
neurobehavioural
function
animals,
performance
group
comparable
control
group.
Histological
examination
further
revealed
tissue
morphology
closely
resembled
These
findings
provide
compelling
evidence
platelet-derived
accessible
treatment
TBI.
investigation
is
needed
determine
long-term
efficacy
underlying
mechanisms
novel
approach.
Journal of Translational Medicine,
Год журнала:
2025,
Номер
23(1)
Опубликована: Май 21, 2025
With
the
discovery
of
intercellular
mitochondrial
transfer,
intricate
regulatory
networks
on
stem
cell
fate
have
aroused
intense
academic
interest.
Apart
from
capturing
freely
released
mitochondria
donor
cells,
cells
are
able
to
receive
through
tunneling
nanotubes
(TNTs),
gap
junctional
channels
(GJCs)
and
extracellular
vesicles
(EVs),
especially
when
undergoing
stressful
conditions
such
as
inflammation,
hypoxia,
chemotherapy
drug
exposure,
irradiation.
Stem
that
potentiated
by
exogenous
show
enhanced
potential
for
proliferation,
differentiation,
immunomodulation.
The
well-tolerated
nature
either
autogenous
or
allogenous
locally
injected
in
human
ischemic
heart
has
validated
safety
therapeutic
transplantation.
In
children
diagnosed
with
DNA
deletion
syndrome,
functional
improvements
been
observed
empowering
their
hematopoietic
maternally
derived
mitochondria.
widely
investigated
applications
transfer
ischemia-reperfusion
injury,
neurodegenerative
diseases
etc.,
potentials
tissue
repair
regeneration
equally
noteworthy,
though
there
no
systematic
summary
this
regard.This
review
analyzed
research
development
trends
regenerative
medicine
over
past
decade
a
bibliometric
perspective,
introduced
concept
associated
mechanisms
summarized
regulations
fate.
Finally,
application
transplantation
reviewed,
including
recent
clinical
studies
related
transplantation.Mitochondrial
shows
promise
modifying
reshaping
cellular
properties
making
them
more
conducive
regeneration.
Mesenchymal
(MSCs)-derived
shown
multifaceted
promoting
revitalization
cardiac,
cutaneous,
muscular,
neuronal
tissue.
This
integrates
novel
findings
biology
medicine,
emphasizing
crucial
translational
value
It
serves
underscore
significant
impact
provides
valuable
reference
further
exploration
field.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 23, 2024
Abstract
Mitochondria,
known
as
the
powerhouse
of
cell,
are
essential
for
cellular
energy
production.
Dysfunction
in
mitochondrial
function
can
significantly
affect
various
organs.
Transplanting
healthy
mitochondria
enhance
bioenergetics
diseased
cells
and
treat
conditions,
yet
limits
transplantation
still
unknown.
Our
study
reveals
that
source
transplanted
is
not
restricted
by
species,
recipient
show
no
significant
immune
response
to
from
different
lineages.
We
also
found
metabolic
compatibility
between
exogenous
crucial,
transplanting
species
endow
with
distinct
characteristics
combat
diseases.
Furthermore,
our
data
indicate
there
competition
among
varying
functions,
more
powerful
yielding
better
therapeutic
effects.
Notably,
we
have
an
upper
limit
bio-enhancement
provided
mitochondria.
research
proposes
a
feasible
path
human
through
transplantation—adaptive
bio-enhancement.
Mitochondrial
highly
clinically
valuable
technique.
I
further
expanded
variety
sources
demonstrated
potential
adaptively
biological
functions.
