Frontiers in Malaria, Год журнала: 2024, Номер 2
Опубликована: Ноя. 28, 2024
Introduction Extracellular vesicles (EVs) are lipid bilayer membrane-enclosed nanoparticles, secreted by all cell types. Information regarding EVs and their molecular cargo in gestational parasitic infections, particularly those caused Plasmodium soil-transmitted helminths (STH), remains largely unexplored. This study aimed to perform isolation characterization of plasma-derived from Colombian pregnant women compare quantity, size, concentration protein according the infectious status, investigate if parasite-derived proteins could be detected as biological circulating infected with , STH co-infections. Materials methods A descriptive 5 groups was performed: 1) Pregnant infection (n=10). 2) (n=14). 3) coinfection 4) without nor 5) Non-pregnant (n=6). Plasma-derived were isolated size exclusion chromatography (SEC) fractions containing identified a bead-based flow cytometric assay for CD9; quantified nanoparticle tracking analysis, associated liquid chromatography-mass spectrometry pool samples per group. Results There no statistical differences expression CD9 marker among groups. The range more variable three (100-700 nm) compared uninfected (50-300 nm). total 823 quantifiable measurable abundance values within five Of proteins, 758 human, six pertained P. vivax fifteen Trichiuris trichiura one hookworms. Data available via ProteomeXchange identifier PXD051270. Discussion is first that identifies women. identification such neglected tropical parasites accounting major burden disease worldwide, open possibilities studying physiological role during infections well exploring them antigen discovery, vaccine development biomarker discovery.
Язык: Английский