Proteomic profile of plasma-derived extracellular vesicles from Colombian pregnant women with Plasmodium-soil transmitted helminths coinfection DOI Creative Commons
Jahnnyer Martínez-Moreno, Alberto Ayllon-Hermida, Berta Barnadas-Carceller

et al.

Frontiers in Malaria, Journal Year: 2024, Volume and Issue: 2

Published: Nov. 28, 2024

Introduction Extracellular vesicles (EVs) are lipid bilayer membrane-enclosed nanoparticles, secreted by all cell types. Information regarding EVs and their molecular cargo in gestational parasitic infections, particularly those caused Plasmodium soil-transmitted helminths (STH), remains largely unexplored. This study aimed to perform isolation characterization of plasma-derived from Colombian pregnant women compare quantity, size, concentration protein according the infectious status, investigate if parasite-derived proteins could be detected as biological circulating infected with , STH co-infections. Materials methods A descriptive 5 groups was performed: 1) Pregnant infection (n=10). 2) (n=14). 3) coinfection 4) without nor 5) Non-pregnant (n=6). Plasma-derived were isolated size exclusion chromatography (SEC) fractions containing identified a bead-based flow cytometric assay for CD9; quantified nanoparticle tracking analysis, associated liquid chromatography-mass spectrometry pool samples per group. Results There no statistical differences expression CD9 marker among groups. The range more variable three (100-700 nm) compared uninfected (50-300 nm). total 823 quantifiable measurable abundance values within five Of proteins, 758 human, six pertained P. vivax fifteen Trichiuris trichiura one hookworms. Data available via ProteomeXchange identifier PXD051270. Discussion is first that identifies women. identification such neglected tropical parasites accounting major burden disease worldwide, open possibilities studying physiological role during infections well exploring them antigen discovery, vaccine development biomarker discovery.

Language: Английский

Understanding the Extracellular Vesicles in Helminths DOI
Antonio Marcilla, Christian M. Sánchez‐López, Aránzazu González

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Spectral flow cytometry for detecting DNA cargo in malaria parasite-derived extracellular vesicles DOI Creative Commons
Ewa Kozela, Ekaterina Petrovich-Kopitman,

Yves Berger

et al.

Journal of Biological Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 108481 - 108481

Published: April 1, 2025

Cells across biological kingdoms release extracellular vesicles (EVs) as a means of communication with other cells, be their friends or foes. This is indeed true for the intracellular malaria parasite Plasmodium falciparum (Pf), which utilizes EVs to transport bioactive molecules various human host systems. Yet, study this mode in research currently constrained due limitations high-resolution tools and absence commercial antibodies. Here, we demonstrate power an advanced spectral flow cytometry approach robustly detect secreted EVs, isolated from Pf-infected red blood cells. By labeling both EV membrane lipids DNA cargo within (non-antibody staining approach), were able subpopulation parasitic-derived enriched DNA. Furthermore, could quantitatively measure DNA-carrying two distinct stages parasite: rings trophozoites. Our findings showcase potential monitor dynamic changes nucleic acid pathogenic EVs.

Language: Английский

Citations

0
Pasteurization and lyophilization affect membrane proteins of pomegranate-derived nanovesicles reducing their functional properties and cellular uptake DOI
Mari Cruz Manzaneque-López, Aránzazu González, Pedro Pérez‐Bermúdez

et al.

Food Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 144303 - 144303

Published: April 1, 2025

Language: Английский

Citations

0
Proteomic profile of plasma-derived extracellular vesicles from Colombian pregnant women with Plasmodium-soil transmitted helminths coinfection DOI Creative Commons
Jahnnyer Martínez-Moreno, Alberto Ayllon-Hermida, Berta Barnadas-Carceller

et al.

Frontiers in Malaria, Journal Year: 2024, Volume and Issue: 2

Published: Nov. 28, 2024

Introduction Extracellular vesicles (EVs) are lipid bilayer membrane-enclosed nanoparticles, secreted by all cell types. Information regarding EVs and their molecular cargo in gestational parasitic infections, particularly those caused Plasmodium soil-transmitted helminths (STH), remains largely unexplored. This study aimed to perform isolation characterization of plasma-derived from Colombian pregnant women compare quantity, size, concentration protein according the infectious status, investigate if parasite-derived proteins could be detected as biological circulating infected with , STH co-infections. Materials methods A descriptive 5 groups was performed: 1) Pregnant infection (n=10). 2) (n=14). 3) coinfection 4) without nor 5) Non-pregnant (n=6). Plasma-derived were isolated size exclusion chromatography (SEC) fractions containing identified a bead-based flow cytometric assay for CD9; quantified nanoparticle tracking analysis, associated liquid chromatography-mass spectrometry pool samples per group. Results There no statistical differences expression CD9 marker among groups. The range more variable three (100-700 nm) compared uninfected (50-300 nm). total 823 quantifiable measurable abundance values within five Of proteins, 758 human, six pertained P. vivax fifteen Trichiuris trichiura one hookworms. Data available via ProteomeXchange identifier PXD051270. Discussion is first that identifies women. identification such neglected tropical parasites accounting major burden disease worldwide, open possibilities studying physiological role during infections well exploring them antigen discovery, vaccine development biomarker discovery.

Language: Английский

Citations

0