Astilbin attenuates apoptosis induced by cadmium through oxidative stress in carp (Cyprinus carpio L.) head kidney lymphocyte

Fish & Shellfish Immunology, Год журнала: 2022, Номер 125, С. 230 - 237

Опубликована: Май 13, 2022

Язык: Английский

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Luteolin Mitigates D-Galactose-Induced Brain Ageing in Rats: SIRT1-Mediated Neuroprotection

Neurochemical Research, Год журнала: 2024, Номер 49(10), С. 2803 - 2820

Опубликована: Июль 11, 2024

Abstract Luteolin is an essential natural polyphenol found in a variety of plants. Numerous studies have supported its protective role neurodegenerative diseases, yet the research for therapeutic utility D-galactose (D-gal)-induced brain ageing still lacking. In this study, potential neuroprotective impact luteolin against D-gal-induced was explored. Forty rats were randomly divided into four groups: control, luteolin, D-gal, and luteolin-administered D-gal groups. All groups subjected to behavioural, cholinergic function, hippocampal mitochondrial respiration assessments. Hippocampal oxidative, neuro-inflammatory, senescence apoptotic indicators detected. Gene expressions SIRT1, BDNF, RAGE assessed. histopathological studies, along with GFAP Ki67 immunoreactivity, performed. Our results demonstrated that effectively alleviated cognitive impairment reversed abnormalities. Furthermore, administration substantially mitigated hippocampus oxidative stress, dysfunction, neuro-inflammation, triggered by D-gal. Additionally, treatment considerably attenuated neuronal apoptosis upregulated SIRT1 mRNA expression. conclusion, our findings revealed D-gal-evoked senescence, improving function enhancing neuroregeneration rat model through antioxidant, senolytic, anti-inflammatory, anti-apoptotic impacts, possibly due upregulation SIRT1. could be promising modality aging-associated

Язык: Английский

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Ginseng under forest exerts stronger anti-aging effects compared to garden ginseng probably via regulating PI3K/AKT/mTOR pathway, SIRT1/NF-κB pathway and intestinal flora

Phytomedicine, Год журнала: 2022, Номер 105, С. 154365 - 154365

Опубликована: Июль 27, 2022

Язык: Английский

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Hyperbaric oxygen therapy for healthy aging: From mechanisms to therapeutics

Redox Biology, Год журнала: 2022, Номер 53, С. 102352 - 102352

Опубликована: Май 27, 2022

Hyperbaric oxygen therapy (HBOT), a technique through which 100% is provided at pressure higher than 1 atm absolute (ATA), has become well-established treatment modality for multiple conditions. The noninvasive nature, favorable safety profile, and common clinical application of HBOT make it competitive candidate several new indications, one them being aging age-related diseases. In fact, despite the conventional wisdom that excessive accelerates aging, appropriate protocols without exceeding toxicity threshold have shown great promise in therapies against aging. For thing, an extensive body basic research expanded our mechanistic understanding HBOT. Interestingly, therapeutic targets overlap considerably with those another, pre-clinical small-scale investigations validated information on efficacy from various aspects. However, generally applicable protocol to be utilized needs defined as subsequent step. It high time look back summarize recent advances concerning biological mechanisms implications promoting healthy shed light prospective directions. Here we provide first comprehensive overview field geriatric research, allows scientific community aware emerging tendency move beyond findings translational value.

Язык: Английский

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Lactobacillus plantarum Combined with Galactooligosaccharides Supplement: A Neuroprotective Regimen Against Neurodegeneration and Memory Impairment by Regulating Short-Chain Fatty Acids and the c-Jun N-Terminal Kinase Signaling Pathway in Mice

Journal of Agricultural and Food Chemistry, Год журнала: 2022, Номер 70(28), С. 8619 - 8630

Опубликована: Июль 11, 2022

Probiotics and prebiotics have received attention in alleviating neurodegenerative diseases. Lactobacillus plantarum (L. plantarum) 69-2 was combined with galactooligosaccharides (GOS) supplemented a d-galactose (d-gal)-induced neurodegeneration memory impairment mice model to explore its effects on the brain regulation of short-chain fatty acids. The results showed that L. plantarum-GOS supplementation inhibited d-gal-induced oxidative stress increased brain's nuclear factor erythroid 2-related 2 (Nrf2) levels. Butyrate, metabolite gut microbiota regulated by GOS, inhibits p-JNK expression, downregulates pro-apoptotic proteins expression activation inflammatory mediators, upregulates synaptic protein expression. This might be potential mechanism for GOS alleviate impairment. study sheds new light development aging-related neuroprotective dietary supplements based gut–brain axis.

Язык: Английский

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Bisphenol A aggravates renal apoptosis and necroptosis in selenium‐deficient chickens via oxidative stress and PI3K/AKT pathway

Journal of Cellular Physiology, Год журнала: 2022, Номер 237(8), С. 3292 - 3304

Опубликована: Май 26, 2022

Abstract Bisphenol A (BPA) in the environment can have deleterious effects on humans and animals. BPA exert nephrotoxicity by inducing oxidative stress. Selenium (Se) deficiency specifically impair kidney tissues additionally show a synergistic effect toxicity of several environmental chemicals. However, toxic chicken whether Se produces remain poorly understood. Herein, we established exposure models model vivo vitro, described discovery path aggravation apoptosis necroptosis Se‐deficient kidneys via regulation stress phosphatidylinositol 3‐kinase/threonine kinase (PI3K/AKT) signaling pathway. We found that increased reactive oxygen species malondialdehyde levels, reduced activities catalase, GPx, superoxide dismutase, downregulated PI3K AKT expressions, activated Bcl/Bax‐Caspase 9‐Caspase 3, receptor‐interacting protein 1/mixed lineage domain‐like pathways, resulting kidney. In addition, significantly promoted expression renal BPA‐exposed kidneys. Altogether, our results showed aggravates PI3K/AKT Our findings elucidate mechanism exacerbation chickens will provide great significance for protection ecological animal health.

Язык: Английский

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Oral Administration of Deer Bone Collagen Peptide Can Enhance the Skin Hydration Ability and Antioxidant Ability of Aging Mice Induced by D-Gal, and Regulate the Synthesis and Degradation of Collagen

Nutrients, Год журнала: 2024, Номер 16(11), С. 1548 - 1548

Опубликована: Май 21, 2024

Skin problems caused by aging have attracted much attention, and marine collagen peptides been proved to improve these problems, while mammalian are rarely reported. In this study, fermented deer bone peptide (FCP) non-fermented (NCP) were extracted from bone, respectively, their sequences differential proteins analyzed using LC-MS/MS technology. After they applied mice induced with D-gal, the skin hydration ability, antioxidant synthesis, degradation ability of studied. The results show that FCP NCP mainly constitute type Ⅰ collagen, segments different. vivo experiments can richness fibers in mice; skin; promote activity antioxidant-related enzymes; also through TGF-β MAPK pathways, synthesis regulated. These aging, capacity mice, regulate pathway.

Язык: Английский

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Hydrogen sulfide inhibits skeletal muscle ageing by up‐regulating autophagy through promoting deubiquitination of adenosine 5’‐monophosphate (AMP)‐activated protein kinase α1 via ubiquitin specific peptidase 5

Journal of Cachexia Sarcopenia and Muscle, Год журнала: 2024, Номер 15(5), С. 2118 - 2133

Опубликована: Авг. 27, 2024

Abstract Background Hydrogen sulfide (H 2 S), the third gasotransmitter discovered, regulates a variety of physiological functions. Whether H S alleviates skeletal muscle ageing by regulating autophagy has not been reported. Methods Mice were administered 150 mg/kg/day D ‐galactose ( ‐gal), and C2C12 myotubes cultured in 20 g/L ‐gal to induce ageing. Sodium hydrosulfide (NaHS) was employed as an exogenous donor treatment group. The intracellular concentration quantified 7‐azido‐4‐methylcoumarin fluorescence probe. proteins involved ubiquitin‐mediated degradation AMPKα1 detected liquid chromatography tandem mass spectrometry (LC–MS/MS) co‐immunoprecipitation (Co‐IP). S‐sulfhydration USP5 tested biotin‐switch assay. Associated analysed western blot. Results NaHS found effectively restore content both gastrocnemius (+91.89%, P < 0.001) (+27.55%, 0.001). In comparison D‐gal group, observed increase mean cross‐sectional area fibres (+44.91%, 0.001), decrease collagen volume fraction (−81.32%, = reduce β‐galactosidase‐positive (−28.74%, also reverse expression atrophy F box protein (MAFbx), muscle‐specific RING finger 1 (MuRF1), Cyclin D1 p21 tissue (MAFbx: −31.73%, 0.008; MuRF1: −32.37%, 0.003; D1: +45.34%, 0.010; p21: −25.53%, 0.022) −16.38%, 0.001; −16.45%, +40.23%, −35.85%, 0.026). AMPKα1–ULK1 pathway activated up‐regulated NaHS‐treated (p‐AMPKα1: +61.61%, 0.018; AMPKα1: +30.64%, p‐ULK1/ULK1: +85.87%, 0.005; p62: −29.07%, Beclin1: +24.75%, 0.007; light chain 3 II/I [LC3 II/I]: +55.78%, 0.004) +77.49%, +26.18%, 0.022; +38.34%, 0.012; −9.02%, 0.014; +13.36%, LC3 II/I: +79.38%, 0.017; flux: +24.88%, 0.034) compared with effects on comparable those acadesine LYN‐1604, chloroquine could its LC–MS/MS Co‐IP experiments demonstrated that is deubiquitinating enzyme AMPKα1. Following knockdown USP5, activation decreased −42.10%, −43.93%, inhibited (p‐ULK1/ULK1: −27.51, +36.00, −22.15%, lost ability up‐regulate autophagy. (gastrocnemius: +62.17%, myotubes: +37.51%, 0.003) (+53.07%, 0.009) ubiquitination Conclusions promotes deubiquitination increasing thereby up‐regulating alleviating

Язык: Английский

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20(S)‐protopanaxatriol inhibited D‐galactose‐induced brain aging in mice via promoting mitochondrial autophagy flow

Phytotherapy Research, Год журнала: 2023, Номер 37(7), С. 2827 - 2840

Опубликована: Апрель 10, 2023

Previous reports have confirmed that saponins (ginsenosides) derived from Panax ginseng. C. A. Meyer exerted obvious memory-enhancing and antiaging effects, the simpler structure of ginsenosides, better biological activity. In this work, we aimed to explore therapeutic effect underlying molecular mechanism 20(S)-protopanaxatriol (PPT), aglycone panaxatriol-type by establishing D-galactose (D-gal)-induced subacute brain aging model in mice. The results showed PPT treatment (10 20 mg/kg) for 4 weeks could significantly restore D-gal (800 mg/kg 8 weeks)-induced impaired memory function, choline dysfunction, redox system imbalance Meanwhile, also reduced histopathological changes caused exposure. Moreover, increase TFEB/LAMP2 protein expression promote mitochondrial autophagic flow. Importantly, docking had good binding ability with LAMP2 TFEB, suggesting might play an important role alleviate D-gal-caused aging.

Язык: Английский

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Lactoferrin ameliorates cognitive impairment in D-galactose-induced aging mice by regulating the PI3K/Akt/mTOR signaling pathway and the microbiome-gut-brain axis

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 143033 - 143033

Опубликована: Апрель 1, 2025

Язык: Английский

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