Novel Usefulness of M2BPGi for Predicting Severity and Clinical Outcomes in Hospitalized COVID-19 Patients

Diagnostics, Год журнала: 2025, Номер 15(7), С. 937 - 937

Опубликована: Апрель 6, 2025

Background/Objectives: Mac-2 binding protein glycosylation isomer (M2BPGi) is a novel biomarker for liver fibrosis, and its prognostic role has never been explored in coronavirus disease 2019 (COVID-19). We compared the M2BPGi level simultaneously with age, severe/critical disease, sequential organ failure assessment (SOFA) score, National Early Warning Score 2 (NEWS2) total of 53 hospitalized patients COVID-19 (mild/moderate [n = 15] 38]). Methods: levels were measured using HISCL assay (Sysmex, Kobe, Japan) an HISCL-5000 analyzer (Sysmex), clinical outcomes analyzed according to variables, receiver operating characteristic (ROC) curve, Kaplan-Meier survival, Cox proportional hazards regression analyses. Results: differed significantly severity, 30-day mortality, 60-day mortality (p 0.045, 0.011, 0.002, respectively). In ROC curve analysis, M2BPGi, SOFA NEWS2, except predicted (all p < 0.01). survival hazard ratios added each variable higher than that alone, was only independent factor mortality. Conclusions: This study demonstrated may be useful assessing severity patients. Combined conventional assessment, would provide objective valuable information prognosis prediction these critically ill Further studies are warranted extend utility other settings.

Язык: Английский

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Discovery of Cinnamoyl–Flavonoid Hybrid Derivatives as Inhibitors of SARS-CoV-2 Mpro and Anti-inflammatory Agents: Experimental and In Silico Insights into their Efficacy against Lipopolysaccharide-Induced Lung Injury

European Journal of Pharmacology, Год журнала: 2025, Номер unknown, С. 177636 - 177636

Опубликована: Апрель 1, 2025

The chemical structures of the parental compounds flavonoids from Boesenbergia rotunda were modified by conjugation with cinnamic acid to form cinnamoyl-flavonoid hybrid derivatives enhanced anti-inflammatory and SARS-CoV-2 Mpro-inhibitory properties. Cinnamoyl-flavonoid 6 10 showed potential inhibit Mpro IC50 values 52.49 22.62 μM. Compounds lower cytotoxicity in human lung cell lines MRC-5 A549 at concentrations greater than 50 effects on viability studied a 3D co-culture model treated lipopolysaccharide (LPS) observed through confocal microscopy. downregulated p65 mRNA expression, resulting reduction pro-inflammatory cytokines, including Interleukin 8 (IL-8) Monocyte Chemoattractant Protein-1 (MCP-1/CCL2), leading an response Nuclear factor kappa-light-chain-enhancer activated B cells (NF-κB) signalling pathways. Compound activity, downregulating Bcl-2 Associated X gene (BAX), which resulted inhibition apoptotic death when compared compound 10. In silico molecular dynamic simulation shed light how these interact myeloid differentiation 2 (MD-2), is involved inflammatory response. Our findings suggest that show as drugs anti-SARS-CoV-2 drugs.

Язык: Английский

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Predictive Biomarkers of Acute Kidney Injury in COVID-19: Distinct Inflammatory Pathways in Patients with and Without Pre-Existing Chronic Kidney Disease

Life, Год журнала: 2025, Номер 15(5), С. 720 - 720

Опубликована: Апрель 29, 2025

Background: Acute kidney injury (AKI) has emerged as a significant complication in patients with coronavirus disease 2019 (COVID-19). The pathophysiology of COVID-19-associated AKI is multifactorial, involving both direct viral effects on renal cells and indirect mechanisms such systemic inflammation cytokine storms. This highlights the critical need for early detection effective management strategies to mitigate improve patient outcomes. aim our study assess potential predictive role inflammatory biomarkers determining risk developing without pre-existing CKD. Methods: included 84 stratified by chronic (CKD) status. Demographic, clinical, laboratory data were collected, including vital signs, hematological profiles, function markers, biomarkers, coagulation parameters, treatments. Outcomes acute in-hospital mortality documented. Results: In CKD, IL-6 NLR demonstrated high accuracy onset. white blood cell (WBC) count predictor Conclusions: differential roles IL-6, NLR, WBC predicting onset highlight distinct physiopathological pathways influenced COVID-19. CKD+ patients, immune dysregulation are key drivers AKI, serving robust markers this state. contrast, CKD− may be more responses infectious factors, reflected count.

Язык: Английский

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Association of Vitamin D Supplementation with Glutathione Peroxidase (GPx) Activity, Interleukine-6 (IL-6) Levels, and Anxiety and Depression Scores in Patients with Post-COVID-19 Condition

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(10), С. 4582 - 4582

Опубликована: Май 10, 2025

Coronavirus disease 2019 (COVID-19) presents with various symptoms, and some patients develop post-COVID-19 condition (PCC). Vitamin D has shown therapeutic potential in COVID-19 may offer benefits for PCC. The aim of this study was to evaluate the differences associated two supplementation strategies (bolus daily) on interleukin-6 (IL-6) levels, glutathione peroxidase (GPx) activity, clinical outcomes PCC patients, regardless whether target 25 (OH) levels reached ideal range. We conducted a self-controlled which 54 participants were supplemented vitamin D3 (n = 28 bolus n 26 2 months. Blood samples collected measure IL-6 GPx activity using spectrophotometric methods. Hospital Anxiety Depression Scale (HADS) used assess mental function. Both daily significantly increased decreased levels. Daily additionally significant reduction anxiety depression scores. However, neither regimen improvements cough, dyspnea, or fatigue. These findings suggest association between antioxidant neuropsychiatric parameters PCC, possibly mediated by its immunomodulatory properties. Further placebo-controlled trials are warranted determine these observed associations reflect causal relationships.

Язык: Английский

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Cardiopulmonary function alterations in mild and moderate SARS-CoV-2 patients: a longitudinal comparison of pre-infection and early recovery phases

Virology Journal, Год журнала: 2025, Номер 22(1)

Опубликована: Май 15, 2025

The purpose of this study is to compare and analyze the changes CPET pulmonary function indexes in patients with mild moderate SARS-CoV-2 before infection early recovery period, explore influence on cardiopulmonary fitness its pathogenesis. Clinical data 39 cases are collected, paired analyses ventilation parameters after performed using software SPSS. Bivariate correlations analyzed for days post-infection, VO2peak decline rate, VO2peak/kg infection, AHRR residual symptom count. results show that VO2peak, VO2peak/kg, AT significantly decreased infection. VE/VCO2 slope increased, while PetCO2, VEpeak, minimum showed reductions. FVC, FEV1, FEV1/FVC remained unchanged. OUES declined, along HRpeak, although no significant differences observed HRrest, HRR-1 min, HRR-2 min. number symptoms correlated but not duration. Additionally, rate strongly associated post-infection time VO2peak/kg. VO2/HR power also significantly. Moreover, function, including cardiac chronotropic muscle impaired patients. Residual closely linked function. Given large proportion cases, these findings offer valuable insights developing targeted interventions prevent further progression improve health population.

Язык: Английский

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Reactivation of Latent Tuberculosis Following COVID-19 and Epstein-Barr Virus Coinfection: A Case Report

Pathogens, Год журнала: 2025, Номер 14(5), С. 488 - 488

Опубликована: Май 16, 2025

Background: This case is unique in demonstrating the reactivation of latent tuberculosis (TB) following co-infection with SARS-CoV-2 and Epstein–Barr virus (EBV) an otherwise healthy young adult. It highlights a rare clinical scenario which viral immune dysregulation likely facilitated TB progression. To date, few reports have explored complex interplay between COVID-19, EBV reactivation, single patient, particularly isolated extrapulmonary involvement. Case Presentation: A 24-year-old woman presented persistent low-grade fever, fatigue, night sweats, unintentional weight loss, progressive cervical supraclavicular lymphadenopathy. These symptoms emerged shortly after moderate COVID-19 infection. Laboratory studies revealed elevated inflammatory markers pronounced lymphopenia. was confirmed via serology PCR. Despite antiviral therapy, persisted, imaging necrotic Tuberculous lymphadenitis diagnosed through fine-needle aspiration cytology PCR detection Mycobacterium tuberculosis. The patient treated standard anti-tuberculosis regimen, resulting clinical, radiological, immunological improvement. Conclusions: underscores importance considering patients lymphadenopathy recent infections, regions high prevalence. also emphasizes need for thorough microbiological assessment post-viral syndromes. main takeaway that may create permissive environment system compromise.

Язык: Английский

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Glutamine Deficiency Promotes Immune and Endothelial Cell Dysfunction in COVID-19

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(8), С. 7593 - 7593

Опубликована: Апрель 20, 2023

The coronavirus disease 2019 (COVID-19) pandemic has caused the death of almost 7 million people worldwide. While vaccinations and new antiviral drugs have greatly reduced number COVID-19 cases, there remains a need for additional therapeutic strategies to combat this deadly disease. Accumulating clinical data discovered deficiency circulating glutamine in patients with that associates severity. Glutamine is semi-essential amino acid metabolized plethora metabolites serve as central modulators immune endothelial cell function. A majority glutamate ammonia by mitochondrial enzyme glutaminase (GLS). Notably, GLS activity upregulated COVID-19, favoring catabolism glutamine. This disturbance metabolism may provoke dysfunction contributes development severe infection, inflammation, oxidative stress, vasospasm, coagulopathy, which leads vascular occlusion, multi-organ failure, death. Strategies restore plasma concentration glutamine, its metabolites, and/or downstream effectors, conjunction drugs, represent promising approach function prevent occlusive stricken COVID-19.

Язык: Английский

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A Post-Pandemic Enigma: The Cardiovascular Impact of Post-Acute Sequelae of SARS-CoV-2

Circulation Research, Год журнала: 2023, Номер 132(10), С. 1358 - 1373

Опубликована: Май 11, 2023

COVID-19 has become the first modern-day pandemic of historic proportion, affecting >600 million individuals worldwide and causing >6.5 deaths. While acute infection had devastating consequences, postacute sequelae SARS-CoV-2 appears to be a its own, impacting up one-third survivors often symptoms suggestive cardiovascular phenomena. This review will highlight suspected pathophysiology SARS-CoV-2, influence on system, potential treatment strategies.

Язык: Английский

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Respiratory disorders of post-COVID-19 syndrome

Terapevticheskii arkhiv, Год журнала: 2023, Номер 95(3), С. 203 - 209

Опубликована: Апрель 26, 2023

Aim. Assess the functional state of trespiratory system and effectiveness therapeutic tactics for broncho-obstructive syndrome (BOS) in patients post-COVID period. Materials methods. A two-center cohort prospective study included 10 456 89 patients, respectively. comprehensive assessment respiratory clinical, laboratory data, spirometry, body plethysmography, a diffusive capacity lungs (DLCO). Therapy consisted budesonide suspension or fixed combination beclomethasone dipropionate/formoterol (EMD BDP/FORM). Results. The frequency BOS was 72% (7497 patients). In 13% (n=974) cases, bronchial asthma diagnosed first time, 4.4% (n=328) chronic obstructive pulmonary disease. Risk factors development decrease DLCO period were identified. group complex instrumental examination lung function, absence violations spirometric data indicators determined by plethysmography determined. Conclusion. are atopy, history frequent acute infections, smoking, blood eosinophilia, moderate severe forms COVID-19. advantage EMD BDP/FORM MART mode compared with nebulized + solution salbutamol treatment shown. disorders established: COVID-19, hospitalization intensive care unit, need additional oxygen therapy.

Язык: Английский

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De novo lupus nephritis after SARS-CoV-2 infection

Lupus, Год журнала: 2023, Номер 32(7), С. 893 - 899

Опубликована: Май 12, 2023

The relationship between viral infection and onset of autoimmune diseases such as systemic lupus erythematosus remains uncertain. During the COVID-19 pandemic, organ-specific multisystemic phenomena temporally related to have been described. Immune dysregulation triggered by SARS-CoV-2 virus leading hyperactivation both innate adaptive immune systems contributes excessive production pro-inflammatory cytokines, autoantibodies, subsequent manifestations. We report two patients without known who developed nephritis shortly after a documented mild infection. Together with other similar cases in literature, observation supports trigger development susceptible individuals.

Язык: Английский

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