Novel Usefulness of M2BPGi for Predicting Severity and Clinical Outcomes in Hospitalized COVID-19 Patients

Diagnostics, Journal Year: 2025, Volume and Issue: 15(7), P. 937 - 937

Published: April 6, 2025

Background/Objectives: Mac-2 binding protein glycosylation isomer (M2BPGi) is a novel biomarker for liver fibrosis, and its prognostic role has never been explored in coronavirus disease 2019 (COVID-19). We compared the M2BPGi level simultaneously with age, severe/critical disease, sequential organ failure assessment (SOFA) score, National Early Warning Score 2 (NEWS2) total of 53 hospitalized patients COVID-19 (mild/moderate [n = 15] 38]). Methods: levels were measured using HISCL assay (Sysmex, Kobe, Japan) an HISCL-5000 analyzer (Sysmex), clinical outcomes analyzed according to variables, receiver operating characteristic (ROC) curve, Kaplan-Meier survival, Cox proportional hazards regression analyses. Results: differed significantly severity, 30-day mortality, 60-day mortality (p 0.045, 0.011, 0.002, respectively). In ROC curve analysis, M2BPGi, SOFA NEWS2, except predicted (all p < 0.01). survival hazard ratios added each variable higher than that alone, was only independent factor mortality. Conclusions: This study demonstrated may be useful assessing severity patients. Combined conventional assessment, would provide objective valuable information prognosis prediction these critically ill Further studies are warranted extend utility other settings.

Language: Английский

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Discovery of Cinnamoyl–Flavonoid Hybrid Derivatives as Inhibitors of SARS-CoV-2 Mpro and Anti-inflammatory Agents: Experimental and In Silico Insights into their Efficacy against Lipopolysaccharide-Induced Lung Injury

European Journal of Pharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 177636 - 177636

Published: April 1, 2025

The chemical structures of the parental compounds flavonoids from Boesenbergia rotunda were modified by conjugation with cinnamic acid to form cinnamoyl-flavonoid hybrid derivatives enhanced anti-inflammatory and SARS-CoV-2 Mpro-inhibitory properties. Cinnamoyl-flavonoid 6 10 showed potential inhibit Mpro IC50 values 52.49 22.62 μM. Compounds lower cytotoxicity in human lung cell lines MRC-5 A549 at concentrations greater than 50 effects on viability studied a 3D co-culture model treated lipopolysaccharide (LPS) observed through confocal microscopy. downregulated p65 mRNA expression, resulting reduction pro-inflammatory cytokines, including Interleukin 8 (IL-8) Monocyte Chemoattractant Protein-1 (MCP-1/CCL2), leading an response Nuclear factor kappa-light-chain-enhancer activated B cells (NF-κB) signalling pathways. Compound activity, downregulating Bcl-2 Associated X gene (BAX), which resulted inhibition apoptotic death when compared compound 10. In silico molecular dynamic simulation shed light how these interact myeloid differentiation 2 (MD-2), is involved inflammatory response. Our findings suggest that show as drugs anti-SARS-CoV-2 drugs.

Language: Английский

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Predictive Biomarkers of Acute Kidney Injury in COVID-19: Distinct Inflammatory Pathways in Patients with and Without Pre-Existing Chronic Kidney Disease

Life, Journal Year: 2025, Volume and Issue: 15(5), P. 720 - 720

Published: April 29, 2025

Background: Acute kidney injury (AKI) has emerged as a significant complication in patients with coronavirus disease 2019 (COVID-19). The pathophysiology of COVID-19-associated AKI is multifactorial, involving both direct viral effects on renal cells and indirect mechanisms such systemic inflammation cytokine storms. This highlights the critical need for early detection effective management strategies to mitigate improve patient outcomes. aim our study assess potential predictive role inflammatory biomarkers determining risk developing without pre-existing CKD. Methods: included 84 stratified by chronic (CKD) status. Demographic, clinical, laboratory data were collected, including vital signs, hematological profiles, function markers, biomarkers, coagulation parameters, treatments. Outcomes acute in-hospital mortality documented. Results: In CKD, IL-6 NLR demonstrated high accuracy onset. white blood cell (WBC) count predictor Conclusions: differential roles IL-6, NLR, WBC predicting onset highlight distinct physiopathological pathways influenced COVID-19. CKD+ patients, immune dysregulation are key drivers AKI, serving robust markers this state. contrast, CKD− may be more responses infectious factors, reflected count.

Language: Английский

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Glutamine Deficiency Promotes Immune and Endothelial Cell Dysfunction in COVID-19

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(8), P. 7593 - 7593

Published: April 20, 2023

The coronavirus disease 2019 (COVID-19) pandemic has caused the death of almost 7 million people worldwide. While vaccinations and new antiviral drugs have greatly reduced number COVID-19 cases, there remains a need for additional therapeutic strategies to combat this deadly disease. Accumulating clinical data discovered deficiency circulating glutamine in patients with that associates severity. Glutamine is semi-essential amino acid metabolized plethora metabolites serve as central modulators immune endothelial cell function. A majority glutamate ammonia by mitochondrial enzyme glutaminase (GLS). Notably, GLS activity upregulated COVID-19, favoring catabolism glutamine. This disturbance metabolism may provoke dysfunction contributes development severe infection, inflammation, oxidative stress, vasospasm, coagulopathy, which leads vascular occlusion, multi-organ failure, death. Strategies restore plasma concentration glutamine, its metabolites, and/or downstream effectors, conjunction drugs, represent promising approach function prevent occlusive stricken COVID-19.

Language: Английский

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A Post-Pandemic Enigma: The Cardiovascular Impact of Post-Acute Sequelae of SARS-CoV-2

Circulation Research, Journal Year: 2023, Volume and Issue: 132(10), P. 1358 - 1373

Published: May 11, 2023

COVID-19 has become the first modern-day pandemic of historic proportion, affecting >600 million individuals worldwide and causing >6.5 deaths. While acute infection had devastating consequences, postacute sequelae SARS-CoV-2 appears to be a its own, impacting up one-third survivors often symptoms suggestive cardiovascular phenomena. This review will highlight suspected pathophysiology SARS-CoV-2, influence on system, potential treatment strategies.

Language: Английский

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Respiratory disorders of post-COVID-19 syndrome

Terapevticheskii arkhiv, Journal Year: 2023, Volume and Issue: 95(3), P. 203 - 209

Published: April 26, 2023

Aim. Assess the functional state of trespiratory system and effectiveness therapeutic tactics for broncho-obstructive syndrome (BOS) in patients post-COVID period. Materials methods. A two-center cohort prospective study included 10 456 89 patients, respectively. comprehensive assessment respiratory clinical, laboratory data, spirometry, body plethysmography, a diffusive capacity lungs (DLCO). Therapy consisted budesonide suspension or fixed combination beclomethasone dipropionate/formoterol (EMD BDP/FORM). Results. The frequency BOS was 72% (7497 patients). In 13% (n=974) cases, bronchial asthma diagnosed first time, 4.4% (n=328) chronic obstructive pulmonary disease. Risk factors development decrease DLCO period were identified. group complex instrumental examination lung function, absence violations spirometric data indicators determined by plethysmography determined. Conclusion. are atopy, history frequent acute infections, smoking, blood eosinophilia, moderate severe forms COVID-19. advantage EMD BDP/FORM MART mode compared with nebulized + solution salbutamol treatment shown. disorders established: COVID-19, hospitalization intensive care unit, need additional oxygen therapy.

Language: Английский

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De novo lupus nephritis after SARS-CoV-2 infection

Lupus, Journal Year: 2023, Volume and Issue: 32(7), P. 893 - 899

Published: May 12, 2023

The relationship between viral infection and onset of autoimmune diseases such as systemic lupus erythematosus remains uncertain. During the COVID-19 pandemic, organ-specific multisystemic phenomena temporally related to have been described. Immune dysregulation triggered by SARS-CoV-2 virus leading hyperactivation both innate adaptive immune systems contributes excessive production pro-inflammatory cytokines, autoantibodies, subsequent manifestations. We report two patients without known who developed nephritis shortly after a documented mild infection. Together with other similar cases in literature, observation supports trigger development susceptible individuals.

Language: Английский

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Genes, inflammatory response, tolerance, and resistance to virus infections in migratory birds, bats, and rodents

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: Aug. 29, 2023

Normally, the host immunological response to viral infection is coordinated restore homeostasis and protect individual from possible tissue damage. The two major approaches are adopted by deal with pathogen: resistance or tolerance. nature of responses often differs between species individuals same species. Resistance includes innate adaptive immune control virus replication. Disease tolerance relies on allowing coexistence infections in minimal no clinical signs, while maintaining sufficient replication for transmission. Here, we compared virome bats, rodents migratory birds molecular mechanisms underlying symptomatic asymptomatic disease progression. We also explore influence physiology environmental influences RNA expression how it impacts whole brain transcriptome seemingly healthy semipalmated sandpiper ( Calidris pusilla ) spotted Actitis macularius ). Three time points throughout year were selected understand importance longitudinal surveys characterization virome. finally revisited evidence that upstream downstream regulation inflammatory is, respectively, associated infections.

Language: Английский

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Comparison of plasma mitochondrial DNA copy number in asymptomatic and symptomatic COVID-19 patients

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Oct. 6, 2023

Introduction Since the beginning of COVID-19 pandemic, a wide clinical spectrum, from asymptomatic infection to mild or severe disease and death, have been reported in patients. Studies suggested several possible factors, which may affect outcome COVID-19. A pro-inflammatory state impaired antiviral response as major contributing factors Considering that mitochondria an important role regulating immune responses pathogens, signaling, cell it has received much attention SARS-CoV-2 infection. Recent studies demonstrated high levels cell-free mitochondrial DNA ( cf -mtDNA) are associated with increased risk intensive care unit (ICU) admission mortality. However, there few on -mtDNA infection, mainly focusing critically ill cases. In present study, we investigated copy number patients compared between symptomatic cases, assessed values. We also determined -nuclear -nDNA) transcription factor (TFAM) mRNA level studied groups. Materials methods Plasma buffy coat samples were collected 37 33 controls. Briefly, after total extraction, plasma -mtDNA, -nDNA numbers measured by absolute qPCR using standard curve method. Furthermore, RNA extraction cDNA synthesis, TFAM evaluated qPCR. Results The results showed statistically significantly higher than cases p value = 0.01). 0.00). There was no significant difference these two groups &gt; 0.05). Also, good diagnostic potential subgroups. Conclusion is probably due its inflammation response. It can be promising candidate biomarker for diagnosis Further investigation will help understanding pathophysiology effective therapeutic strategies.

Language: Английский

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Innate immune activation and white matter injury in a rat model of neonatal intraventricular hemorrhage are dependent on developmental stage

Experimental Neurology, Journal Year: 2023, Volume and Issue: 367, P. 114472 - 114472

Published: June 17, 2023

Inflammation and white matter injury are consequences of neonatal intraventricular hemorrhage (IVH). Both the neuroimmune system developing during time which IVH occurs its develop. has been studied in many different animal models; however, effects occurring at developmental points same model have not examined. Understanding how timing affects outcome may provide important insights into both pathophysiology innate immune development. We used injection lysed whole blood to postnatal day (P)2 P5 rats. Flow cytometry was detect activation. MRI screen animals for development increased ventricular size. Immunohistochemistry myelin basic protein quantify corpus callosum thickness. exhibited significant increases several measures classically pro-inflammatory activation that P2 did not. Animals with induced also developed enlargement visible on whereas On histological analysis, there were no animals, but reduced labeling induces a strong inflammatory response as well changes size reduction matter. do exhibit or structure after IVH. This suggests pathology from is due part activation; stage key determinant pathology.

Language: Английский

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