Laminarin Alleviates Acute Lung Injury Induced by LPS Through Inhibition of M1 Macrophage Polarisation

Journal of Cellular and Molecular Medicine, Год журнала: 2025, Номер 29(5)

Опубликована: Март 1, 2025

The lipopolysaccharide-induced acute lung injury (ALI) mouse model is used to simulate human respiratory distress syndrome (ARDS), which has a high mortality rate. An imbalance between M1 and M2 macrophages, characterised by an increase in was observed sepsis-induced ALI. We report that laminarin, active ingredient found algae, exhibits exceptional performance of It ameliorates edema, enhances the survival rate mice reduces levels inflammatory factors TNF-α IL-6. Furthermore, laminarin reduced expression CD86, are markers associated with macrophages. Laminarin treatment secretion IL-6 LPS-stimulated also decreases glucose uptake Transcriptome sequencing reveals genes downregulated macrophages following predominantly enriched HIF-1α signalling pathway. Experimental validation confirms significantly related indicators ROS NLRP3. After using siRNA knock down RAW264.7 cells, inhibitory effect on LPS-induced polarisation abolished. This suggests may potentially inhibit macrophage towards phenotype downregulating signal. In conclusion, data presented our study demonstrate can effectively reduce signalling. makes it novel candidate drug for

Язык: Английский

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Association between rTMS-induced changes in inflammatory markers and improvement in psychiatric diseases: a systematic review

Annals of General Psychiatry, Год журнала: 2024, Номер 23(1)

Опубликована: Авг. 27, 2024

Repetitive transcranial magnetic stimulation (rTMS) has recently gained relevance in treating different psychiatric disorders. Limited evidence suggests that the beneficial effects of rTMS on psychopathology could be at least partly mediated through changes inflammatory response. This systematic review summarizes literature whether can modulate markers and thus positively influence course illnesses. A diseases was conducted according to PRISMA guidelines. Information association between treatment response extracted. The quality studies assessed using National Heart, Lung, Blood Institute for human Systematic Review Center Laboratory Animal Experimentation animal studies. includes 17 (2 15 studies) relationship markers. Positive microglial activity anti-inflammatory were associated with behavioral improvement models depression. However, these findings have not been consistently replicated focusing treatment-resistant While several reported rTMS-induced alterations peripheral markers, only two demonstrate their clinical Notably, most showed poor or moderate bias assessment. certain suggest an psychopathology, heterogeneity, underpowered analyses constrain generalizability results. discrepancy highlights need larger, standardized (PROSPERO Registration: CRD42023492732).

Язык: Английский

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Acute and long-term cognitive impairment following sepsis: mechanism and prevention

Expert Review of Neurotherapeutics, Год журнала: 2023, Номер 23(10), С. 931 - 943

Опубликована: Авг. 24, 2023

ABSTRACTIntroduction Sepsis is a severe host response to infection, which induces both acute and long-term cognitive impairment. Despite its high incidence following sepsis, the underlying mechanisms remain elusive effective treatments are not available clinically.Area covered This review focuses on elucidating pathological impairment sepsis. Specifically, authors discuss role of systemic inflammation response, blood–brain barrier disruption, neuroinflammation, mitochondrial dysfunction, neuronal Aβ accumulation tau phosphorylation in after Additionally, they current strategies ameliorate impairment.Expert opinion Potential interventions reduce sepsis include earlier diagnosis infection control, hemodynamic homeostasis, adequate brain perfusion. Furthermore, inflammatory reactive oxygen species, injury or death could be beneficial. Implementing minimize delirium, sleep disturbance, stress factors, immobility also recommended. avoiding neurotoxins implementing early rehabilitation may important for preventing sepsis.KEYWORDS: Sepsiscognitive impairmentpathological mechanismsoutcomesinterventions Article highlights impairments.The sepsis-induced fully understood.Effective therapeutics clinically.Interventions associated with might stabilizing hemodynamics perfusion.Interventions attenuate inflammation, beneficial.Avoiding crucial.Abbreviations BBB=Blood-brain barrier;BDNF=brain-derived neurotrophic factor;CLP=Cecum ligation puncture;CNS=Central nervous system;EEG=Electroencephalogram;HMGB1=High mobility group protein 1;HMG-CoA=Hydroxymethylglutaryl coenzyme A;HO-1=Heme oxygenase-1;ICU=Intensive care unit;IFNs=Interferons;IL=Interleukin;LPS=Lipopolysaccharide;MMPs=Matrix metalloproteinases;NOX2=NADPH oxidase isoform 2;NF-κB=Nuclear factor kappa B;NLRP3=Nucleotide-binding domain-like receptor protein3;NRG1=Neuregulin-1;Nrf2=Nuclear erythroid-2 related factor;proBDNF=precursor form brain-derived factor;PV=parvalbumin;ROS=Reactive species;SAE=Sepsis-associated encephalopathy;TLRs=Toll-like receptors.Declaration interestThe have no other relevant affiliations financial involvement any organization entity interest conflict subject matter materials discussed manuscript apart from those disclosed.Reviewer disclosuresPeer reviewers this relationships disclose.Additional informationFundingThis work was supported by grants National Natural Science Foundation China (81971892 & 82172131).

Язык: Английский

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Analysis of blood biochemistry and non-targeted metabolomics of endometritis in dairy cows

Animal Reproduction Science, Год журнала: 2024, Номер 264, С. 107460 - 107460

Опубликована: Март 30, 2024

Язык: Английский

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Rosuvastatin mitigates blood–brain barrier disruption in sepsis-associated encephalopathy by restoring occludin levels

European journal of medical research, Год журнала: 2025, Номер 30(1)

Опубликована: Фев. 14, 2025

Blood–brain barrier (BBB) disruption is a key pathological feature of sepsis-associated encephalopathy (SAE). Rosuvastatin, third-generation statin, exhibits diverse pharmacological functions beyond its lipid-lowering capacity. However, potential neuroprotective role in SAE remains unclear. models were established using the cecal ligation and puncture (CLP) method. BBB integrity was evaluated NaF, endothelial permeability assessed by fluorescein isothiocyanate (FITC)-dextran assays. Rosuvastatin significantly attenuated neuroinflammation brains septic mice reducing expression pro-inflammatory cytokines interleukin-6 (IL-6), monocyte chemoattractant protein-1 (MCP-1), tumor necrosis factor α (TNF-α). It also ameliorated vascular injury brain cortex decreasing levels cell adhesion molecule-1 (VCAM-1) E-selectin. Furthermore, preserved enhancing tight junction protein occludin. In vitro studies demonstrated that alleviated increased transendothelial electrical resistance (TEER) lipopolysaccharide (LPS)-stimulated human microvascular cells (HBMECs). Additionally, prevented LPS-induced reduction occludin Krüppel-like 2 (KLF2) HBMECs. Importantly, silencing KLF2 abrogated Rosuvastatin's protective effects on expression. These findings indicate may be promising therapeutic candidate for mitigating dysfunction associated with SAE.

Язык: Английский

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Low levels of CD39+ Tregs may predict poor outcome in children with sepsis

International Immunopharmacology, Год журнала: 2025, Номер 153, С. 114445 - 114445

Опубликована: Март 21, 2025

Язык: Английский

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Effective management of pediatric septic shock: a case study utilizing continuous renal replacement therapy with cytosorb and citrate in a leukemic patient with hyper-interleukin (IL)-6-naemia and severe thrombocytopenia

BMC Infectious Diseases, Год журнала: 2025, Номер 25(1)

Опубликована: Март 26, 2025

Sepsis is a critical condition characterized by dysregulated immune response to infection, often resulting in organ dysfunction. Interleukin-6 (IL-6) key proinflammatory cytokine associated with sepsis and its complications. This case study explored the use of Continuous Renal Replacement Therapy (CRRT) combined Cytosorb managing pediatric patients leukemia, severe thrombocytopenia, elevated IL-6 levels. A 10-year-old boy Fanconi anemia presented pancytopenia, fever, necrotic lesions, indicative mucormycosis. Following diagnosis acute myeloid leukemia (AML), patient experienced complications, including septic shock. Despite appropriate treatment, inflammatory markers, such as C-reactive protein (CRP), procalcitonin (PCT), IL-6, were significantly elevated. CRRT was initiated manage hypercytokinemia improve patient's clinical status. The procedure utilizes citrate an anticoagulant because thrombocytopenia. treatment lasted for 20 h, during which biomarkers monitored. post-treatment results indicated significant reduction levels within 24 h decrease PCT at 8 h. CRP gradually declined over 40 exhibited marked improvement, healing cutaneous lesions stabilization allowing transfer ward continued chemotherapy. suggests that cytosorb may be valuable adjunctive therapy complex cases observed particularly warrants further investigation. Concurrent antimicrobial, antifungal, dexamethasone treatments IRIS have influenced outcomes this single case. Controlled studies are needed evaluate specific contribution Cytosorb-CRRT assess long-term efficacy safety similar populations, resource-limited settings. Not applicable.

Язык: Английский

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Ex Vivo Plasma Application on Human Brain Microvascular Endothelial-like Cells for Blood–Brain Barrier Modeling

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(7), С. 3334 - 3334

Опубликована: Апрель 3, 2025

hiPSC-derived blood-brain barrier (BBB) models are valuable for pharmacological and physiological studies, yet their translational potential is limited due to insufficient cell phenotypes the neglection of complex environment BBB. This study evaluates plasma compatibility with microvascular endothelial-like cells enhance in vitro BBB models. Therefore, samples (sodium/lithium heparin, citrate, EDTA) serum from healthy donors were tested on at concentrations 100%, 75%, 50%. After 24 h, viability parameters assessed. The impact heparin-anticoagulated plasmas was further evaluated regarding function endothelial phenotype differentiated cells. Finally, sodium-heparin an isogenic triple-culture model continuous TEER measurements 72 h. Only application did not significantly alter compared medium. Furthermore, heparin improved without increasing density induced a von Willebrand factor signal. confirmed positive function. Consequently, proven be compatible Thereby, can substantially future.

Язык: Английский

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Endothelial Control of the Inflammatory Process

Inflammation, Год журнала: 2025, Номер unknown, С. 1 - 31

Опубликована: Янв. 1, 2025

Язык: Английский

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Decoding mechanisms and protein markers in lung-brain axis

Respiratory Research, Год журнала: 2025, Номер 26(1)

Опубликована: Май 19, 2025

The lung-brain axis represents a complex bidirectional communication network that is pivotal in the crosstalk between respiratory and neurological functions. This review summarizes current understanding of mechanisms protein markers mediate effects lung diseases on brain health. In this review, we explore linking injury to neurocognitive impairments, focusing neural pathways, immune regulation inflammatory responses, microorganism hypoxemia. Specifically, highlight role vagus nerve modulating central nervous system response pulmonary stimuli; Additionally, regulatory function underscored, with evidence suggesting lung-derived mediators can traverse blood-brain barrier, induce neuroinflammation cognitive decline; Furthermore, discuss potential microbiota influence through microbial translocation activation; Finally, impact hypoxemia examined, findings indicating it exacerbate cerebral via oxidative stress impaired perfusion. Moreover, analyze how conditions, such as pneumonia, ALI/ARDS, asthma, contribute dysfunction. Prolonged mechanical ventilation also impairment. Conversely, (e.g., stroke, traumatic injury) lead acute complications. addition, TLR4, ACE2, A-SAA, HMGB1, TREM2 are crucial correlate disease severity. We emerging therapeutic strategies targeting axis, including immunomodulation microbiome engineering. Overall, interplay for developing integrated treatment improving patient outcomes. Further research needed elucidate molecular foster interdisciplinary collaboration.

Язык: Английский

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