Ginsenoside Rb1-loaded bionic nanoparticles alleviate sepsis-induced acute lung injury by reducing mitochondrial oxidative stress to inhibit macrophage PANoptosis DOI Creative Commons

Shujuan Wu,

Huifan Liu, Xu Liu

и другие.

Materials & Design, Год журнала: 2024, Номер 245, С. 113291 - 113291

Опубликована: Сен. 1, 2024

Cytokin storms play a key role in the pathogenesis of acute lung injury (ALI) caused by sepsis, which pulmonary macrophage PANoptosis is involved. At present, main treatment methods for sepsis-induced ALI are antibiotics and fluid resuscitation, but efficacy these unsatisfactory enough; therefore, development new therapeutic urgently needed. In this study, we developed novel bionic nanoparticle (R@ZQC NPs) using ZIF-8 nanoparticles as carrier, internally supported with ginsenoside Rb1, externally coated quaternated chitosan membrane layer layer. The biomimetic controlled release had good biocompatibility, strong antibacterial activity, targeted inhibition PANoptosis. vitro vivo, R@ZQC NPs effectively inhibited activity Escherichia coli reduced mitochondrial damage activating AMPK pathway, thus alleviating inflammatory response ameliorating ALI. summary, study revealed great potential antimicrobial reduction represents first attempt This provides ideas strategies clinical

Язык: Английский

Acute lung injury: a view from the perspective of necroptosis DOI

Jinyan Dong,

Weihong Liu, Wenli Liu

и другие.

Inflammation Research, Год журнала: 2024, Номер 73(6), С. 997 - 1018

Опубликована: Апрель 14, 2024

Язык: Английский

Процитировано

7
Exploring the Anti-PANoptosis Mechanism of Dachaihu Decoction Against Sepsis-Induced Acute Lung Injury: Network Pharmacology, Bioinformatics, and Experimental Validation DOI Creative Commons
Zhen Yang,

Xingyu Kao,

Lin Zhang

и другие.

Drug Design Development and Therapy, Год журнала: 2025, Номер Volume 19, С. 349 - 368

Опубликована: Янв. 1, 2025

Dachaihu decoction (DCHD) is a common Chinese medicine formula against sepsis-induced acute lung injury (SALI). PANoptosis novel type of programmed cell death. Nevertheless, The mechanisms DCHD SALI via anti-PANoptosis remains unknown. First, we identified the intersecting targets among DCHD, SALI, and using relevant databases published literature. Then, protein-protein interaction (PPI) network, molecular docking, functional enrichment analysis were conducted. In vivo, cecal ligation puncture (CLP) was used to construct sepsis mouse model, therapeutic effects on evaluated hematoxylin eosin (H&E) staining, quantitative real-time PCR (qRT-PCR), ELISA. Finally, qRT-PCR, immunofluorescence Western blotting verify effect DCHD-containing serum (DCHD-DS) LPS-induced RAW 264.7 macrophages in vitro. 82 by mapping PANoptosis. Enrichment showed that modulating tumor necrosis factor (TNF), AGE-RAGE, phosphoinositide 3-kinase (PI3K)-AKT, Toll-like receptor signaling pathways targeting Casp3, cellular antigen p53 (TP53), B-cell lymphoma 2 (Bcl2), toll-like receptor-4 (TLR4), STAT3, STAT1, RELA, NF-κB1, myeloid leukemia-1 (MCL1), JUN, IL-1β, HSP90AA1, Casp9, Casp8, Bcl2l1. Molecular docking revealed key components have high binding affinity core targets. improved histopathological injury, reduced inflammatory expression, alleviated oxidative stress tissues. vitro, DCHD-DS morphology changes, release pro-inflammatory factors, p65 nucleus aggregation. Furthermore, verified inhibited downregulating PI3K/AKT/NF-κB signalling pathway. attenuates inhibiting control Our study provides solid foundation for investigating its clinical application treatment SALI.

Язык: Английский

Процитировано

1
Cordyceps militaris solid medium extract alleviates lipopolysaccharide-induced acute lung injury via regulating gut microbiota and metabolism DOI Creative Commons
Xiaoya Wang, Kang Zhang,

Jingyan Zhang

и другие.

Frontiers in Immunology, Год журнала: 2025, Номер 15

Опубликована: Янв. 20, 2025

Acute lung injury (ALI) is a common respiratory disease, Cordycepin has been reported to reduce ALI, which an effective component in Cordyceps militaris solid medium extract (CMME). Therefore, we aimed explore the alleviating effect and mechanism of CMME on ALI. This study evaluated lipopolysaccharide (LPS)-induced ALI mice by analyzing intestinal flora metabolomics its potential mechanism. We assessed pulmonary changes, inflammation, oxidative stress, macrophage neutrophil activation levels, then analyzed gut microbiota through 16S rRNA profile UPLC-QTOF/MS. The results showed that treatment improved injury, reduced inflammatory factors stress decreased recruitment. revealed significantly increased richness diversity abundance Bacteroides compared with Mod group significantly. Metabolic analysis indicated reversed levels differential metabolites may ameliorate purine metabolism, nucleotide bile acid (BA) did reverse changes BA mice, were associated flora. improve regulating correcting metabolic disorders, providing new insights into action.

Язык: Английский

Процитировано

1
cGAS-STING targeting offers therapy choice in lung diseases DOI Creative Commons
Yu Wang, X. Zhang, Weixue Wang

и другие.

Biology Direct, Год журнала: 2025, Номер 20(1)

Опубликована: Фев. 7, 2025

Cyclic GMP/AMP (cGAMP) synthase (cGAS), along with the endoplasmic reticulum (ER)-associated stimulator of interferon genes (STING), are crucial elements type 1 response. cGAS senses microbial DNA and self-DNA, labeling cGAS-STING as a mechanism in autoimmunity, sterile inflammatory responses, cellular senescence. However, chronic aberrant activation axis results autoimmune diseases. has emerged vital driving inflammation-related diseases, including lung Insights into biology pathway have enabled discovery small-molecule agents which potential to inhibit In this review, we first outline principal components signaling cascade. Then, discuss recent research that highlights general mechanisms by contributes focus on summarizing list bioactive compounds pathway, reviewing their mechanisms.These review novel groundbreaking therapeutic possibilities through targeting

Язык: Английский

Процитировано

1
Ursodeoxycholic and chenodeoxycholic bile acids alleviate endotoxininduced acute lung injury in rats by modulating aquaporin expression and pathways associated with apoptosis and inflammation DOI Creative Commons

Tatjana Milivojac,

Milkica Grabež,

Ljiljana Amidžić

и другие.

Frontiers in Pharmacology, Год журнала: 2025, Номер 16

Опубликована: Март 6, 2025

Introduction This study aimed to investigate the anti-inflammatory, antioxidant, and anti-apoptotic properties of ursodeoxycholic (UDCA) chenodeoxycholic (CDCA) bile acids in a rat model endotoxin (lipopolysaccharide, LPS)-induced acute lung injury (ALI). Methods The included six groups Wistar rats exposed different pretreatments. control were pretreated with propylene glycol, solvent for acids, while other received UDCA or CDCA 10 days. On 10th day, an injection was given evaluate impact these Lung tissue sections analyzed by immunohistochemistry, targeting pro-inflammatory marker nuclear factor kappa B (NF-κB), B-cell lymphoma 2 (BCL-2), pro-apoptotic markers BCL-2-associated X protein (BAX) caspase 3, as well aquaporins 1 5 (AQP1 AQP5). Oxidative stress assessed bronchoalveolar lavage fluid (BALF). Results discussion demonstrates that can mitigate endotoxin-induced rats. These effects are achieved through modulation AQP1 AQP5 expression, reduction oxidative stress, regulation apoptotic pathways (BAX, BCL-2), attenuation activity NF-κB. Although results indicate significant association between expression proteins histopathological changes, potential influence additional factors cannot be excluded. findings suggest provide protection acting complex mechanisms involving inflammatory, oxidative, pathways.

Язык: Английский

Процитировано

1
Generation of Anti‐Mastitis Gene‐Edited Dairy Goats with Enhancing Lysozyme Expression by Inflammatory Regulatory Sequence using ISDra2‐TnpB System DOI Creative Commons
Rui Feng, Gang Zhao, Qian Zhang

и другие.

Advanced Science, Год журнала: 2024, Номер 11(38)

Опубликована: Авг. 5, 2024

Gene-editing technology has become a transformative tool for the precise manipulation of biological genomes and holds great significance in field animal disease-resistant breeding. Mastitis, prevalent disease husbandry, imposes substantial economic burden on global dairy industry. In this study, regulatory sequence gene editing breeding strategy successful creation gene-edited (GED) goats with enhanced mastitis resistance using ISDra2-TnpB system as model is proposed. This included targeted integration an innate inflammatory (IRS) into promoter region lysozyme (LYZ) gene. Upon Escherichia Coli (E. coli) mammary gland infection, GED exhibited increased LYZ expression, showing robust anti-mastitis capabilities, mitigating PANoptosis activation, alleviating blood-milk-barrier (BMB) damage. Notably, highly expressed only E. coli infection. study marks advent animals exogenous-free expression demonstrates feasibility gene-editing proposed study. addition, it provides novel blueprint developing strains, focusing specificity biosafety while providing research basis widespread application system.

Язык: Английский

Процитировано

6
Dachengqi decoction dispensing granule ameliorates LPS-induced acute lung injury by inhibiting PANoptosis in vivo and in vitro DOI
Mengqi Zhang,

Luorui Shang,

Fangyuan Zhou

и другие.

Journal of Ethnopharmacology, Год журнала: 2024, Номер 336, С. 118699 - 118699

Опубликована: Авг. 23, 2024

Язык: Английский

Процитировано

6
Focus on the cGAS-STING Signaling Pathway in Sepsis and Its Inflammatory Regulatory Effects DOI Creative Commons
Yupeng Han,

Liangcheng Qiu,

Haixing Wu

и другие.

Journal of Inflammation Research, Год журнала: 2024, Номер Volume 17, С. 3629 - 3639

Опубликована: Июнь 1, 2024

Abstract: Sepsis is a severe systemic inflammatory response commonly occurring in infectious diseases, caused by infection with virulent pathogens. In the pathogenesis of sepsis, cyclic guanosine monophosphate (GMP)-adenosine (AMP) synthase-stimulator interferon genes (cGAS-STING) signaling pathway serves crucial role as fundamental immunoregulatory mechanism. This activates STING upon recognizing intracellular DNA damage and pathogen-derived DNA, subsequently inducing production numerous mediators, including cytokines, which turn trigger an response. The aim this paper to explore activation mechanism cGAS-STING sepsis its impact on regulation. By delving into action we identify new therapeutic strategies for treatment prevention sepsis. Keywords: cGAS, STING, inflammation

Язык: Английский

Процитировано

4
BMAL1 plays a crucial role in immune homeostasis during sepsis-induced acute lung injury DOI
Ting Zeng, Long Liang, Wenjun Deng

и другие.

Biochemical Pharmacology, Год журнала: 2024, Номер 226, С. 116379 - 116379

Опубликована: Июнь 21, 2024

Язык: Английский

Процитировано

4
PANoptosis: A new era for anti-cancer strategies DOI
Zilian Cui, Yuan Li,

Yao Bi

и другие.

Life Sciences, Год журнала: 2024, Номер unknown, С. 123241 - 123241

Опубликована: Ноя. 1, 2024

Язык: Английский

Процитировано

3