SIRT1 activation by Ligustrazine ameliorates migraine via the paracrine interaction of microglia and neurons

Phytomedicine, Год журнала: 2024, Номер 135, С. 156069 - 156069

Опубликована: Сен. 17, 2024

Язык: Английский

---

Edaravone Dexborneol provides neuroprotective effect by inhibiting neurotoxic activation of astrocytes through inhibiting NF-κB signaling in cortical ischemia

Brain Research Bulletin, Год журнала: 2024, Номер 218, С. 111097 - 111097

Опубликована: Окт. 10, 2024

Edaravone Dexborneol (EDB), comprised of edaravone and (+)- bornel, has been demonstrated to have synergistic effects antioxidant anti-inflammatory, which makes it be applied for stroke as a protectant. However, the underlying mechanism neuroprotection EDB not fully elucidated. Increasing evidence shown that neurotoxic A1 astrocytes were closely related neuronal death after cerebral ischemia. whether could provide by modulating activation yet The present study aimed explore afforded polarization down-stream signaling We first validated neuroprotective in mice suffering focal ischemia via evaluating behavioral test, infarct volumes survival. As signaling, our data further showed alleviated suppressing inhibition NF-κB pathway vitro. Additionally, administration reduced number reactive above findings provided effect inhibiting animal model ischemia, indicated EDB-mediated phenotypic regulation is potential research direction promote neurological recovery central nervous system (CNS) diseases.

Язык: Английский

---

Targeting astrocytes polarization after spinal cord injury: a promising direction

Frontiers in Cellular Neuroscience, Год журнала: 2024, Номер 18

Опубликована: Окт. 16, 2024

Spinal cord injury (SCI) is a serious neurological that causes severe trauma to motor and sensory functions. Although long considered incurable, recent research has brought new hope for functional recovery from SCI. After SCI, astrocytes are activated into many polarization states. Here we discuss the two most important classical phenotypes: ‘A1’ neurotoxic phenotype ‘A2’ neuroprotective phenotype, with A1 being impeding neurorecovery, A2 neuroprotective. This paper discusses changes in astrocyte responsiveness after SCI pros cons of their It also elucidates feasibility as therapeutic target neuroprotection. In future, multiple intervention strategies targeting expected gain wider clinical application, ultimately improving motor-sensory function quality life patients.

Язык: Английский

---

Enhanced inhibition of neuronal ferroptosis and regulation of microglial polarization with multifunctional traditional Chinese medicine active ingredient-based selenium nanoparticles for treating spinal cord injury

Materials Today Bio, Год журнала: 2025, Номер unknown, С. 101758 - 101758

Опубликована: Апрель 1, 2025

Язык: Английский

---

Terpenoids: A Promising Traditional Chinese Medicine for Neuropathic Pain Relief

Pharmacological Research, Год журнала: 2025, Номер unknown, С. 107789 - 107789

Опубликована: Май 1, 2025

Язык: Английский

---

Ligustrazine Alleviates Blood–Brain Barrier Damage by Downregulating Expression of miR‐297c‐5p

CNS Neuroscience & Therapeutics, Год журнала: 2025, Номер 31(5)

Опубликована: Май 1, 2025

ABSTRACT Objective Ligustrazine (LSZ), an ingredient of Ligusticum chuanxiong , has long been used to treat neurovascular diseases in China. This study investigates its protective effects for the impairment blood–brain barrier (BBB) and underlying mechanisms. Methods In this study, impacts LSZ on BBB function were firstly assessed b. End3 cells vitro. Oxygen–glucose deprivation (OGD) served as injury factor western blot (WB) analyzed expressions occludin ZO‐1, two tight junction proteins (TJs), essential maintaining integrity BBB. After bioinformatics analysis transcriptome vivo, qRT‐PCR miR‐297c‐5p was conducted a dual‐luciferase reporter assay verify target protein, occludin, which confirmed by hippocampal insertion using guide cannulas microinfection RNA oligos. Results A 3‐h OGD resulted noticeable reductions level ZO‐1. However, administration (0.1 μM) effectively restored these decreases. normal mice, (25 mg/kg, i.p., once daily 9 days) notable reduction miR‐297c‐5p. middle cerebral artery occlusion (MCAO) mouse model, increased also reversed administration. Bioinformatics revealed one targets includes occludin. MiR‐297c‐5p found directly assay. Transfection agomir into significant while transfection antagomir led increase Besides, stereotaxic injection AAV‐miR‐297c‐5p hippocampus reduced vivo. Ultimately, oligos provided confirmation that downregulated MCAO mice with up‐regulated expression. Conclusion conclusion, present findings provide new insights regulating through downregulation

Язык: Английский

---

Astragaloside IV Relieves Central Sensitization by Regulating Astrocytic ROS/NF‐κB Nuclear Translocation Signaling in Chronic Migraine Male Rats

Phytotherapy Research, Год журнала: 2025, Номер unknown

Опубликована: Янв. 13, 2025

ABSTRACT Chronic migraine (CM) is a disabling neurological disease. Astragaloside IV (AS‐IV), natural product derived from Astragalus membranaceus , shows great potential in treating chronic pain by relieving inflammation and oxidative stress. This study aimed to investigate the effects mechanisms of action AS‐IV on CM. An inflammatory soup comprising histamine, bradykinin, serotonin, prostaglandin E2 was used establish CM rat model, while lipopolysaccharide applied induce an response primary astrocytes. Pain threshold measurements were evaluate nociceptive hypersensitivity, qPCR Western blotting detect indicators synaptic protein expression, Golgi‐Cox staining observe dendritic spine density, transmission electron microscopy ultrastructure. Mitochondrial function stress assessed using JC‐1 staining, Mitotracker reactive oxygen species (ROS) quantification, glutathione content. pretreatment alleviated central sensitization ameliorated astrocyte activation neuroinflammation. mitochondrial dysfunction vitro, reduced nuclear translocation NF‐κB production IL‐1β, which reversed ROS scavengers vitro or respiratory chain disruptors vivo. Our indicates that can inhibit neuroinflammation alleviating mitigate CM, thereby providing experimental basis for A . prevention treatment.

Язык: Английский

---

Sirtuin1 in Spinal Cord Injury: Regulatory Mechanisms, Microenvironment Remodeling and Therapeutic Potential

CNS Neuroscience & Therapeutics, Год журнала: 2025, Номер 31(2)

Опубликована: Фев. 1, 2025

ABSTRACT Background Spinal cord injury (SCI) is a complex central nervous system disorder characterized by multifaceted pathological processes, including inflammation, oxidative stress, programmed cell death, autophagy, and mitochondrial dysfunction. Sirtuin 1 (Sirt1), critical NAD + ‐dependent deacetylase, has emerged as promising therapeutic target for SCI repair due to its potential protect neurons, regulate glial vascular cells, optimize the microenvironment. However, regulatory roles of Sirt1 in are challenging, effects vary depending on activation timing, expression levels, types. Methods A systematic literature review was conducted using PubMed, Scopus, Web Science identify studies investigating SCI. Relevant publications were analyzed synthesize current evidence Sirt1's mechanisms, effects, challenges repair. Results exerts broad across diverse processes types post‐SCI. It promotes neuronal survival axonal regeneration, modulates astrocytes microglia resolve supports oligodendrocyte‐mediated myelination, enhances endothelial function. Proper may mitigate secondary injury, whereas excessive or prolonged could impair inflammatory resolution disrupt cellular homeostasis. This highlights therapies, but include optimizing spatiotemporal addressing dual different Conclusion Targeting represents viable strategy repair, given regulation neuroprotection, immunomodulation, tissue remodeling. translating these findings into therapies requires resolving issues such type‐specific delivery, precise dosage control. provides theoretical foundation practical insights advancing Sirt1‐based treatments

Язык: Английский

---

Spinal astrocytes involved in the pathogenesis and treatment of neuropathic pain

Frontiers in Cellular Neuroscience, Год журнала: 2025, Номер 19

Опубликована: Фев. 21, 2025

Neuropathic pain is a common and severe type of chronic pain, its pathogenesis has not been fully defined. Increasing evidence shows that spinal astrocytes play indispensable roles in the occurrence development neuropathic pain. Most studies have suggested activated can crosstalk with other glial cells neurons through morphological functional changes, exacerbating However, reactive dual role. As defense mechanism, increasing neuroprotection stimulating neurogenesis. Studies demonstrated potentially beneficial role for astrocyte activation In addition, therapeutic mechanisms multiple drugs neuromodulatory techniques are thought to be related astrocytes. This review highlights recent advances significance astrocytes, emphasizing need better understanding their treatment

Язык: Английский

---

The neurobiology of plant-based therapeutics in women's reproductive health: mechanisms, efficacy, and clinical translation

Frontiers in Nutrition, Год журнала: 2025, Номер 12

Опубликована: Май 20, 2025

This review examines the neurobiological mechanisms by which plant-derived compounds influence women's reproductive health through neuroendocrine-reproductive axis. Gynecological disorders frequently present with neurological manifestations, including cognitive decline in perimenopause, anxiety and depression polycystic ovary syndrome (PCOS), central sensitization endometriosis. Bioactive from medicinal plants, polyphenols phytoestrogens, demonstrate therapeutic potential their anti-inflammatory, antioxidant, neuromodulatory properties. These multi-target offer advantages over conventional single-target therapies simultaneously regulating multiple physiological processes. The explores applications specific gynecological conditions discusses development of dietary supplements functional foods incorporating these ingredients. growing market for products presents opportunities innovative formulations enhanced bioavailability personalized approaches. Future research directions include integrating neuroimaging herbal research, improving clinical translation, establishing regulatory frameworks global application interventions to enhance female health.

Язык: Английский

---