Aging,
Год журнала:
2024,
Номер
16(11), С. 9680 - 9691
Опубликована: Июнь 5, 2024
Objective:
Spinal
cord
injury
(SCI)
is
a
devastating
disease
for
which
there
no
safe
and
effective
treatment
at
present.
Daphnoretin
natural
discoumarin
compound
isolated
from
Wikstroemia
indica
with
various
pharmacological
activities.
Our
study
aimed
to
investigate
the
role
of
in
NF-κB
pathway
activation
inflammatory
response
after
SCI.
Methods:
A
mouse
SCI
model
was
constructed,
Basso
Mouse
Scale
Score
subscore
were
used
evaluate
effect
on
movement
capacity
mice.
The
glial
cells
BV2
observed
by
immunofluorescence.
PCR
ELISA
detect
expression
factors,
Western
blot
performed
protein
associated
pathway.
Results:
inhibited
loss
ability
mice
SCI,
it
also
factors
TNF-α
IL-1β
vivo
vitro.
Conclusions:
can
inhibit
induced
demonstrates
potential
clinical
application
Brain Research Bulletin,
Год журнала:
2024,
Номер
218, С. 111097 - 111097
Опубликована: Окт. 10, 2024
Edaravone
Dexborneol
(EDB),
comprised
of
edaravone
and
(+)-
bornel,
has
been
demonstrated
to
have
synergistic
effects
antioxidant
anti-inflammatory,
which
makes
it
be
applied
for
stroke
as
a
protectant.
However,
the
underlying
mechanism
neuroprotection
EDB
not
fully
elucidated.
Increasing
evidence
shown
that
neurotoxic
A1
astrocytes
were
closely
related
neuronal
death
after
cerebral
ischemia.
whether
could
provide
by
modulating
activation
yet
The
present
study
aimed
explore
afforded
polarization
down-stream
signaling
We
first
validated
neuroprotective
in
mice
suffering
focal
ischemia
via
evaluating
behavioral
test,
infarct
volumes
survival.
As
signaling,
our
data
further
showed
alleviated
suppressing
inhibition
NF-κB
pathway
vitro.
Additionally,
administration
reduced
number
reactive
above
findings
provided
effect
inhibiting
animal
model
ischemia,
indicated
EDB-mediated
phenotypic
regulation
is
potential
research
direction
promote
neurological
recovery
central
nervous
system
(CNS)
diseases.
Frontiers in Cellular Neuroscience,
Год журнала:
2024,
Номер
18
Опубликована: Окт. 16, 2024
Spinal
cord
injury
(SCI)
is
a
serious
neurological
that
causes
severe
trauma
to
motor
and
sensory
functions.
Although
long
considered
incurable,
recent
research
has
brought
new
hope
for
functional
recovery
from
SCI.
After
SCI,
astrocytes
are
activated
into
many
polarization
states.
Here
we
discuss
the
two
most
important
classical
phenotypes:
‘A1’
neurotoxic
phenotype
‘A2’
neuroprotective
phenotype,
with
A1
being
impeding
neurorecovery,
A2
neuroprotective.
This
paper
discusses
changes
in
astrocyte
responsiveness
after
SCI
pros
cons
of
their
It
also
elucidates
feasibility
as
therapeutic
target
neuroprotection.
In
future,
multiple
intervention
strategies
targeting
expected
gain
wider
clinical
application,
ultimately
improving
motor-sensory
function
quality
life
patients.
CNS Neuroscience & Therapeutics,
Год журнала:
2025,
Номер
31(5)
Опубликована: Май 1, 2025
ABSTRACT
Objective
Ligustrazine
(LSZ),
an
ingredient
of
Ligusticum
chuanxiong
,
has
long
been
used
to
treat
neurovascular
diseases
in
China.
This
study
investigates
its
protective
effects
for
the
impairment
blood–brain
barrier
(BBB)
and
underlying
mechanisms.
Methods
In
this
study,
impacts
LSZ
on
BBB
function
were
firstly
assessed
b.
End3
cells
vitro.
Oxygen–glucose
deprivation
(OGD)
served
as
injury
factor
western
blot
(WB)
analyzed
expressions
occludin
ZO‐1,
two
tight
junction
proteins
(TJs),
essential
maintaining
integrity
BBB.
After
bioinformatics
analysis
transcriptome
vivo,
qRT‐PCR
miR‐297c‐5p
was
conducted
a
dual‐luciferase
reporter
assay
verify
target
protein,
occludin,
which
confirmed
by
hippocampal
insertion
using
guide
cannulas
microinfection
RNA
oligos.
Results
A
3‐h
OGD
resulted
noticeable
reductions
level
ZO‐1.
However,
administration
(0.1
μM)
effectively
restored
these
decreases.
normal
mice,
(25
mg/kg,
i.p.,
once
daily
9
days)
notable
reduction
miR‐297c‐5p.
middle
cerebral
artery
occlusion
(MCAO)
mouse
model,
increased
also
reversed
administration.
Bioinformatics
revealed
one
targets
includes
occludin.
MiR‐297c‐5p
found
directly
assay.
Transfection
agomir
into
significant
while
transfection
antagomir
led
increase
Besides,
stereotaxic
injection
AAV‐miR‐297c‐5p
hippocampus
reduced
vivo.
Ultimately,
oligos
provided
confirmation
that
downregulated
MCAO
mice
with
up‐regulated
expression.
Conclusion
conclusion,
present
findings
provide
new
insights
regulating
through
downregulation
Phytotherapy Research,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 13, 2025
ABSTRACT
Chronic
migraine
(CM)
is
a
disabling
neurological
disease.
Astragaloside
IV
(AS‐IV),
natural
product
derived
from
Astragalus
membranaceus
,
shows
great
potential
in
treating
chronic
pain
by
relieving
inflammation
and
oxidative
stress.
This
study
aimed
to
investigate
the
effects
mechanisms
of
action
AS‐IV
on
CM.
An
inflammatory
soup
comprising
histamine,
bradykinin,
serotonin,
prostaglandin
E2
was
used
establish
CM
rat
model,
while
lipopolysaccharide
applied
induce
an
response
primary
astrocytes.
Pain
threshold
measurements
were
evaluate
nociceptive
hypersensitivity,
qPCR
Western
blotting
detect
indicators
synaptic
protein
expression,
Golgi‐Cox
staining
observe
dendritic
spine
density,
transmission
electron
microscopy
ultrastructure.
Mitochondrial
function
stress
assessed
using
JC‐1
staining,
Mitotracker
reactive
oxygen
species
(ROS)
quantification,
glutathione
content.
pretreatment
alleviated
central
sensitization
ameliorated
astrocyte
activation
neuroinflammation.
mitochondrial
dysfunction
vitro,
reduced
nuclear
translocation
NF‐κB
production
IL‐1β,
which
reversed
ROS
scavengers
vitro
or
respiratory
chain
disruptors
vivo.
Our
indicates
that
can
inhibit
neuroinflammation
alleviating
mitigate
CM,
thereby
providing
experimental
basis
for
A
.
prevention
treatment.
CNS Neuroscience & Therapeutics,
Год журнала:
2025,
Номер
31(2)
Опубликована: Фев. 1, 2025
ABSTRACT
Background
Spinal
cord
injury
(SCI)
is
a
complex
central
nervous
system
disorder
characterized
by
multifaceted
pathological
processes,
including
inflammation,
oxidative
stress,
programmed
cell
death,
autophagy,
and
mitochondrial
dysfunction.
Sirtuin
1
(Sirt1),
critical
NAD
+
‐dependent
deacetylase,
has
emerged
as
promising
therapeutic
target
for
SCI
repair
due
to
its
potential
protect
neurons,
regulate
glial
vascular
cells,
optimize
the
microenvironment.
However,
regulatory
roles
of
Sirt1
in
are
challenging,
effects
vary
depending
on
activation
timing,
expression
levels,
types.
Methods
A
systematic
literature
review
was
conducted
using
PubMed,
Scopus,
Web
Science
identify
studies
investigating
SCI.
Relevant
publications
were
analyzed
synthesize
current
evidence
Sirt1's
mechanisms,
effects,
challenges
repair.
Results
exerts
broad
across
diverse
processes
types
post‐SCI.
It
promotes
neuronal
survival
axonal
regeneration,
modulates
astrocytes
microglia
resolve
supports
oligodendrocyte‐mediated
myelination,
enhances
endothelial
function.
Proper
may
mitigate
secondary
injury,
whereas
excessive
or
prolonged
could
impair
inflammatory
resolution
disrupt
cellular
homeostasis.
This
highlights
therapies,
but
include
optimizing
spatiotemporal
addressing
dual
different
Conclusion
Targeting
represents
viable
strategy
repair,
given
regulation
neuroprotection,
immunomodulation,
tissue
remodeling.
translating
these
findings
into
therapies
requires
resolving
issues
such
type‐specific
delivery,
precise
dosage
control.
provides
theoretical
foundation
practical
insights
advancing
Sirt1‐based
treatments
Frontiers in Cellular Neuroscience,
Год журнала:
2025,
Номер
19
Опубликована: Фев. 21, 2025
Neuropathic
pain
is
a
common
and
severe
type
of
chronic
pain,
its
pathogenesis
has
not
been
fully
defined.
Increasing
evidence
shows
that
spinal
astrocytes
play
indispensable
roles
in
the
occurrence
development
neuropathic
pain.
Most
studies
have
suggested
activated
can
crosstalk
with
other
glial
cells
neurons
through
morphological
functional
changes,
exacerbating
However,
reactive
dual
role.
As
defense
mechanism,
increasing
neuroprotection
stimulating
neurogenesis.
Studies
demonstrated
potentially
beneficial
role
for
astrocyte
activation
In
addition,
therapeutic
mechanisms
multiple
drugs
neuromodulatory
techniques
are
thought
to
be
related
astrocytes.
This
review
highlights
recent
advances
significance
astrocytes,
emphasizing
need
better
understanding
their
treatment
Frontiers in Nutrition,
Год журнала:
2025,
Номер
12
Опубликована: Май 20, 2025
This
review
examines
the
neurobiological
mechanisms
by
which
plant-derived
compounds
influence
women's
reproductive
health
through
neuroendocrine-reproductive
axis.
Gynecological
disorders
frequently
present
with
neurological
manifestations,
including
cognitive
decline
in
perimenopause,
anxiety
and
depression
polycystic
ovary
syndrome
(PCOS),
central
sensitization
endometriosis.
Bioactive
from
medicinal
plants,
polyphenols
phytoestrogens,
demonstrate
therapeutic
potential
their
anti-inflammatory,
antioxidant,
neuromodulatory
properties.
These
multi-target
offer
advantages
over
conventional
single-target
therapies
simultaneously
regulating
multiple
physiological
processes.
The
explores
applications
specific
gynecological
conditions
discusses
development
of
dietary
supplements
functional
foods
incorporating
these
ingredients.
growing
market
for
products
presents
opportunities
innovative
formulations
enhanced
bioavailability
personalized
approaches.
Future
research
directions
include
integrating
neuroimaging
herbal
research,
improving
clinical
translation,
establishing
regulatory
frameworks
global
application
interventions
to
enhance
female
health.