Daphnoretin inhibited SCI-induced inflammation and activation of NF-κB pathway in spinal dorsal horn DOI Creative Commons

Jiazhang Wu,

Fengfei Lin, Bin Chen

et al.

Aging, Journal Year: 2024, Volume and Issue: 16(11), P. 9680 - 9691

Published: June 5, 2024

Objective: Spinal cord injury (SCI) is a devastating disease for which there no safe and effective treatment at present. Daphnoretin natural discoumarin compound isolated from Wikstroemia indica with various pharmacological activities. Our study aimed to investigate the role of in NF-κB pathway activation inflammatory response after SCI. Methods: A mouse SCI model was constructed, Basso Mouse Scale Score subscore were used evaluate effect on movement capacity mice. The glial cells BV2 observed by immunofluorescence. PCR ELISA detect expression factors, Western blot performed protein associated pathway. Results: inhibited loss ability mice SCI, it also factors TNF-α IL-1β vivo vitro. Conclusions: can inhibit induced demonstrates potential clinical application

Language: Английский

SIRT1 activation by Ligustrazine ameliorates migraine via the paracrine interaction of microglia and neurons DOI
Yu Chen, Ziwei Xing, Junren Chen

et al.

Phytomedicine, Journal Year: 2024, Volume and Issue: 135, P. 156069 - 156069

Published: Sept. 17, 2024

Language: Английский

Citations

5
Edaravone Dexborneol provides neuroprotective effect by inhibiting neurotoxic activation of astrocytes through inhibiting NF-κB signaling in cortical ischemia DOI Creative Commons
Zhe Chen, Tao Li,

Hai‐Bin Tang

et al.

Brain Research Bulletin, Journal Year: 2024, Volume and Issue: 218, P. 111097 - 111097

Published: Oct. 10, 2024

Edaravone Dexborneol (EDB), comprised of edaravone and (+)- bornel, has been demonstrated to have synergistic effects antioxidant anti-inflammatory, which makes it be applied for stroke as a protectant. However, the underlying mechanism neuroprotection EDB not fully elucidated. Increasing evidence shown that neurotoxic A1 astrocytes were closely related neuronal death after cerebral ischemia. whether could provide by modulating activation yet The present study aimed explore afforded polarization down-stream signaling We first validated neuroprotective in mice suffering focal ischemia via evaluating behavioral test, infarct volumes survival. As signaling, our data further showed alleviated suppressing inhibition NF-κB pathway vitro. Additionally, administration reduced number reactive above findings provided effect inhibiting animal model ischemia, indicated EDB-mediated phenotypic regulation is potential research direction promote neurological recovery central nervous system (CNS) diseases.

Language: Английский

Citations

4
Astragaloside IV Relieves Central Sensitization by Regulating Astrocytic ROS/NF‐κB Nuclear Translocation Signaling in Chronic Migraine Male Rats DOI Open Access
Wei Zhang,

Yunping Yang,

Xiaoyan Zhang

et al.

Phytotherapy Research, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 13, 2025

ABSTRACT Chronic migraine (CM) is a disabling neurological disease. Astragaloside IV (AS‐IV), natural product derived from Astragalus membranaceus , shows great potential in treating chronic pain by relieving inflammation and oxidative stress. This study aimed to investigate the effects mechanisms of action AS‐IV on CM. An inflammatory soup comprising histamine, bradykinin, serotonin, prostaglandin E2 was used establish CM rat model, while lipopolysaccharide applied induce an response primary astrocytes. Pain threshold measurements were evaluate nociceptive hypersensitivity, qPCR Western blotting detect indicators synaptic protein expression, Golgi‐Cox staining observe dendritic spine density, transmission electron microscopy ultrastructure. Mitochondrial function stress assessed using JC‐1 staining, Mitotracker reactive oxygen species (ROS) quantification, glutathione content. pretreatment alleviated central sensitization ameliorated astrocyte activation neuroinflammation. mitochondrial dysfunction vitro, reduced nuclear translocation NF‐κB production IL‐1β, which reversed ROS scavengers vitro or respiratory chain disruptors vivo. Our indicates that can inhibit neuroinflammation alleviating mitigate CM, thereby providing experimental basis for A . prevention treatment.

Language: Английский

Citations

0
Sirtuin1 in Spinal Cord Injury: Regulatory Mechanisms, Microenvironment Remodeling and Therapeutic Potential DOI Creative Commons
Jinze Li, Shengyu Cui, Yanqiu Li

et al.

CNS Neuroscience & Therapeutics, Journal Year: 2025, Volume and Issue: 31(2)

Published: Feb. 1, 2025

ABSTRACT Background Spinal cord injury (SCI) is a complex central nervous system disorder characterized by multifaceted pathological processes, including inflammation, oxidative stress, programmed cell death, autophagy, and mitochondrial dysfunction. Sirtuin 1 (Sirt1), critical NAD + ‐dependent deacetylase, has emerged as promising therapeutic target for SCI repair due to its potential protect neurons, regulate glial vascular cells, optimize the microenvironment. However, regulatory roles of Sirt1 in are challenging, effects vary depending on activation timing, expression levels, types. Methods A systematic literature review was conducted using PubMed, Scopus, Web Science identify studies investigating SCI. Relevant publications were analyzed synthesize current evidence Sirt1's mechanisms, effects, challenges repair. Results exerts broad across diverse processes types post‐SCI. It promotes neuronal survival axonal regeneration, modulates astrocytes microglia resolve supports oligodendrocyte‐mediated myelination, enhances endothelial function. Proper may mitigate secondary injury, whereas excessive or prolonged could impair inflammatory resolution disrupt cellular homeostasis. This highlights therapies, but include optimizing spatiotemporal addressing dual different Conclusion Targeting represents viable strategy repair, given regulation neuroprotection, immunomodulation, tissue remodeling. translating these findings into therapies requires resolving issues such type‐specific delivery, precise dosage control. provides theoretical foundation practical insights advancing Sirt1‐based treatments

Language: Английский

Citations

0
Spinal astrocytes involved in the pathogenesis and treatment of neuropathic pain DOI Creative Commons
Xiaohui Li, Yu‐Shan Huang,

Jinzhu Bai

et al.

Frontiers in Cellular Neuroscience, Journal Year: 2025, Volume and Issue: 19

Published: Feb. 21, 2025

Neuropathic pain is a common and severe type of chronic pain, its pathogenesis has not been fully defined. Increasing evidence shows that spinal astrocytes play indispensable roles in the occurrence development neuropathic pain. Most studies have suggested activated can crosstalk with other glial cells neurons through morphological functional changes, exacerbating However, reactive dual role. As defense mechanism, increasing neuroprotection stimulating neurogenesis. Studies demonstrated potentially beneficial role for astrocyte activation In addition, therapeutic mechanisms multiple drugs neuromodulatory techniques are thought to be related astrocytes. This review highlights recent advances significance astrocytes, emphasizing need better understanding their treatment

Language: Английский

Citations

0
Enhanced inhibition of neuronal ferroptosis and regulation of microglial polarization with multifunctional traditional Chinese medicine active ingredient-based selenium nanoparticles for treating spinal cord injury DOI Creative Commons

Luo-Qi Mai,

Jinggong Liu,

Huimei Wu

et al.

Materials Today Bio, Journal Year: 2025, Volume and Issue: unknown, P. 101758 - 101758

Published: April 1, 2025

Language: Английский

Citations

0
Research Progress on Treating Spinal Cord Injury by Modulating the Phenotype of Microglia DOI Creative Commons

Qinghe Yu,

Ziming Cai, Xiaofeng Liu

et al.

Journal of Integrative Neuroscience, Journal Year: 2024, Volume and Issue: 23(9)

Published: Sept. 19, 2024

Spinal cord injury (SCI) is a severe central nervous system disorder with no currently available effective treatment. Microglia are immune cells in the that play crucial roles SCI occurrence, development, and recovery stages. They exhibit dynamic polarization over time can switch between classical activation (M1) alternative (M2) phenotypes to respond environmental stimuli. The M1 phenotype involved initiating sustaining inflammatory responses, while M2 exerts anti-inflammatory effects promotes tissue repair damaged areas. Inhibiting promoting have become hotspots regulating neuroinflammation treating SCI. This article provides comprehensive review centered on modulating microglial for

Language: Английский

Citations

2
Targeting astrocytes polarization after spinal cord injury: a promising direction DOI Creative Commons

Helin Li,

Ying Liu, Yuxiang Sun

et al.

Frontiers in Cellular Neuroscience, Journal Year: 2024, Volume and Issue: 18

Published: Oct. 16, 2024

Spinal cord injury (SCI) is a serious neurological that causes severe trauma to motor and sensory functions. Although long considered incurable, recent research has brought new hope for functional recovery from SCI. After SCI, astrocytes are activated into many polarization states. Here we discuss the two most important classical phenotypes: ‘A1’ neurotoxic phenotype ‘A2’ neuroprotective phenotype, with A1 being impeding neurorecovery, A2 neuroprotective. This paper discusses changes in astrocyte responsiveness after SCI pros cons of their It also elucidates feasibility as therapeutic target neuroprotection. In future, multiple intervention strategies targeting expected gain wider clinical application, ultimately improving motor-sensory function quality life patients.

Language: Английский

Citations

2
Dopamine D2 Receptor Activation Blocks GluA2/ROS Positive Feedback Loop to Alienate Chronic-Migraine-Associated Pain Sensitization DOI Creative Commons
Wei Zhang, Xiaoyan Zhang, Ming Lei

et al.

Antioxidants, Journal Year: 2024, Volume and Issue: 13(6), P. 725 - 725

Published: June 14, 2024

Chronic migraine is a disabling disorder without effective therapeutic medicine. AMPA receptors have been proven to be essential pathological pain and headaches, but the related regulatory mechanisms in chronic not yet explored. In this study, we found that level of surface GluA2 was reduced rats. Tat-GluR23Y (a endocytosis inhibitor) calcium inward flow weakened synaptic structures, thus alleviating migraine-like sensitization. addition, inhibition influx alleviated mitochondrial overload ROS generation primary neurons. Furthermore, our results showed can induce allodynia rats, promoting previous dopamine D2 receptor identified as potential target treatment migraine, here activation suppressed chronic-migraine-related sensitization through blocking GluA2/ROS positive feedback loop vivo vitro. Additionally, ligustrazine, core component ligusticum chuanxiong, shown receptor, thereby production abnormal nociception CM This study provides valuable insight into migraine.

Language: Английский

Citations

0
Daphnoretin inhibited SCI-induced inflammation and activation of NF-κB pathway in spinal dorsal horn DOI Creative Commons

Jiazhang Wu,

Fengfei Lin, Bin Chen

et al.

Aging, Journal Year: 2024, Volume and Issue: 16(11), P. 9680 - 9691

Published: June 5, 2024

Objective: Spinal cord injury (SCI) is a devastating disease for which there no safe and effective treatment at present. Daphnoretin natural discoumarin compound isolated from Wikstroemia indica with various pharmacological activities. Our study aimed to investigate the role of in NF-κB pathway activation inflammatory response after SCI. Methods: A mouse SCI model was constructed, Basso Mouse Scale Score subscore were used evaluate effect on movement capacity mice. The glial cells BV2 observed by immunofluorescence. PCR ELISA detect expression factors, Western blot performed protein associated pathway. Results: inhibited loss ability mice SCI, it also factors TNF-α IL-1β vivo vitro. Conclusions: can inhibit induced demonstrates potential clinical application

Language: Английский

Citations

0