Astrocyte-derived exosomal miR-378a-5p mitigates cerebral ischemic neuroinflammation by modulating NLRP3-mediated pyroptosis

Frontiers in Immunology, Год журнала: 2024, Номер 15

Опубликована: Авг. 8, 2024

This study aimed to investigate the regulatory role of astrocyte-derived exosomes and their microRNAs (miRNAs) in modulating neuronal pyroptosis during cerebral ischemia.

Язык: Английский

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Effects of edaravone dexborneol on functional outcome and inflammatory response in patients with acute ischemic stroke

BMC Neurology, Год журнала: 2024, Номер 24(1)

Опубликована: Июнь 20, 2024

Abstract Background Edaravone dexborneol has been reported as an effective neuroprotective agent in the treatment of acute ischemic stroke (AIS). This study aimed at investigating impact edaravone on functional outcomes and systematic inflammatory response AIS patient. Methods All participants were recruited from AISRNA (registered 21/11/2019, NCT04175691 [ClinicalTrials.gov]) between January 2022 December 2022. The patients divided into two groups based whether they received (37.5 mg/12 hours, IV) within 48 h after onset. Inflammatory was determined by detecting levels cytokines (interleukin-2 [IL-2], IL-4, IL-5, IL-8, IL-6, IL-10, IL-12p70, IL-17, tumor necrosis factor-α [TNF-α], interferon-γ [IFN-γ], IFN-α, IL-1β) 14 days Results Eighty-five included study. Patients treated with showed a significantly higher proportion modified Rankin Scale score < 2 compared to those who did not receive this (70.7% versus 47.8%; P = 0.031). Furthermore, individuals receiving injection exhibited lower expression interleukin (IL)-1β, along IL-4 IL-10 during phase ( 0.05). These trends observed for IL-2, factor-α, IFN-α > Conclusions Treatment resulted favorable outcome 90 post-stroke onset when without intervention; it also suppressed proinflammatory factors while increasing anti-inflammatory levels. Trial registration ClinicalTrials.gov NCT04175691. Registered November 21, 2019, https://www.clinicaltrials.gov/ct2/show/NCT04175691 .

Язык: Английский

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Glial polarization in neurological diseases: Molecular mechanisms and therapeutic opportunities

Ageing Research Reviews, Год журнала: 2024, Номер 104, С. 102638 - 102638

Опубликована: Дек. 12, 2024

Язык: Английский

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Electroacupuncture alleviates cerebral ischemia injury by regulating PI3K/AKT/NF-κB signaling in microglia of ischemic stroke rats

Neuroreport, Год журнала: 2024, Номер 36(1), С. 22 - 30

Опубликована: Дек. 4, 2024

This study aimed to investigate the potential of electroacupuncture as an intervention for inducing 'Awakening and Opening Brain' in rats with stroke models induced by middle cerebral artery occlusion/reperfusion (MCAO/R). The efficacy alleviating ischemic injury was evaluated using Longa scores, triphenyl tetrazolium chloride staining, hematoxylin eosin staining. Non-targeted metabolomics analysis conducted identify differential metabolite changes before after treatment MCAO/R rats. Network pharmacology then performed correlate these metabolites stroke. PI3K/AKT/NF-κB signaling pathway identified a key target. In vivo experiments further validated mechanism which promotes M2 microglial polarization through inhibition demonstrated that reduces brain damage inhibits inflammation modulating promoting microglia from M1 M2.

Язык: Английский

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Curdione combined with borneol treats bacterial mixed HPV infection by regulating the crosstalk among immune cells

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Янв. 22, 2025

Background Human papillomavirus (HPV) infection is a worldwide reproductive system disease. Baofukang suppository, traditional herbal preparation that includes curdione and borneol, has been reported to treat bacterial vaginosis (BV) HPV in China. However, the therapeutic mechanism still unknown. This study aims explore molecular mechanisms of borneol treating infection. Methods We conducted retrospective cohort analysis medical records from single-center involving 205 patients, focusing on correlation between clearance co-infection with other pathogens, confirming efficacy suppository. Bioinformatics network pharmacology approaches were employed identify targets suppository for BV/HPV co-infections. qRT-PCR, Western blot, immunofluorescence staining, flow cytometry utilized validate along associated immune changes. Finally, confirmed vivo using an LPS/TC-1 cervical orthotopic injection model. Results Curdione selectively inhibit secretion interleukin-6 (IL-6) interleukin-1β (IL-1β) by macrophages. The reduction IL-6 IL-1β levels effectively inhibits expression CD274 (Programmed death ligand 1, PD-L1) infected epithelial cells inhibiting STAT3 phosphorylation, thereby suppressing their evasion capabilities. Furthermore, enhance tumor necrosis factor α (TNF-α) caspase 1 (CASP1) macrophages, as well interleukin 12 (IL-12) 23 (IL-23) dendritic (DCs). these inflammatory factors promotes migration differentiation T site infection, completing cells. Conclusion main components progression occurrence cancer modulating communication innate adaptive immunity, promoting recruitment recognition CD8 + eliminate HPV-infected

Язык: Английский

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Cerebellar Pathology in Forensic and Clinical Neuroscience

Ageing Research Reviews, Год журнала: 2025, Номер unknown, С. 102697 - 102697

Опубликована: Фев. 1, 2025

Язык: Английский

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Edaravone alleviates Pseudomonas aeruginosa associated-acute lung injury by inhibiting inflammation and promoting anti-microbial peptide production

International Immunopharmacology, Год журнала: 2025, Номер 154, С. 114511 - 114511

Опубликована: Март 31, 2025

Язык: Английский

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Edaravone dexborneol protected neurological function by targeting NRF2/ARE and NF-κB/AIM2 pathways in cerebral ischemia/reperfusion injury

Frontiers in Pharmacology, Год журнала: 2025, Номер 16

Опубликована: Апрель 25, 2025

Edaravone dexborneol (Eda-Dex), a promising neuroprotectant composed of edaravone and (+)-borneol, has been clinically applied in stroke treatment. However, the mechanism action Eda-Dex remains unclear. A rat model cerebral ischemia/reperfusion injury (CIRI) was created through middle artery occlusion. Neurological scoring, TTC staining, laser speckle imaging were used to assess neurological deficits, infarct size blood flow (CBF). Behavioral tests, including open field test, elevated plus maze, novel object recognition conducted animal behavior. Western blotting ELISA employed levels expression components NRF2/ARE NF-κB/AIM2 pathways specific cytokines. The oxidative stress markers analyzed via commercially available kits. HE Nissl immunohistochemistry pathological alterations brain. dramatically reduced deficit score size, increased CBF, attenuated anxiety-like behavior improved cognitive function CIRI rats. significantly relieved inflammatory response it upregulated NRF2, NQO1, HO-1, SLC7A11 downregulated NF-κB, AIM2, ASC caspase 1 infarcted Moreover, clearly damage, rescued neurons, activation microglia astrocytes. results this study confirm that exerts neuroprotective effects by synergistically inhibiting inflammation

Язык: Английский

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Dexborneol Amplifies Pregabalin’s Analgesic Effect in Mouse Models of Peripheral Nerve Injury and Incisional Pain

Antioxidants, Год журнала: 2024, Номер 13(7), С. 803 - 803

Опубликована: Июль 2, 2024

Pregabalin is a medication primarily used in the treatment of neuropathic pain and anxiety disorders, owing to its gabapentinoid properties. monotherapy faces limitations due variable efficacy dose-dependent adverse reactions. In this study, we conducted comprehensive investigation into potentiation pregabalin’s analgesic effects by dexborneol, neuroprotective bicyclic monoterpenoid compound. We performed animal experiments where models were induced using two methods: peripheral nerve injury, involving axotomy ligation tibial common peroneal nerves, incisional through longitudinal incision hind paw, while employing multifaceted methodology that integrates behavioral pharmacology, molecular biology, neuromorphology, lipidomics delve mechanisms behind potentiation. Dexborneol was found enhance promoting transportation central nervous system, disrupting self-amplifying vicious cycles via reduction HMGB1 ATP release, exerting significant anti-oxidative modulation lipid metabolism. This combination therapy not only boosted property but also notably decreased side effects. Moreover, therapeutic cocktail exceeded basic relief, effectively reducing neuroinflammation glial cell activation—key factors contributing persistent chronic pain. study paves way for more tolerable effective options, highlighting potential dexborneol as an adjuvant pregabalin therapy.

Язык: Английский

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M1 Microglia-Derived Exosomes Promote A1 Astrocyte Activation and Aggravate Ischemic Injury via circSTRN3/miR-331-5p/MAVS/NF-κB Pathway

Journal of Inflammation Research, Год журнала: 2024, Номер Volume 17, С. 9285 - 9305

Опубликована: Ноя. 1, 2024

After ischemic stroke (IS), microglia and astrocytes undergo polarization, transforming into a pro-inflammatory phenotype (M1 or A1). According to previous studies, exosomes might play an important role in the interplay between M1 A1 after IS.

Язык: Английский

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