Biomaterials that passively and actively target macrophages promote the regeneration of injured tissues

Biomedical Technology, Год журнала: 2024, Номер 8, С. 17 - 49

Опубликована: Окт. 1, 2024

Язык: Английский

---

PBMC-mediated modulation of macrophage polarization in RAW264.7 cells through STAT1/STAT6 signaling cascades

International Immunopharmacology, Год журнала: 2024, Номер 138, С. 112651 - 112651

Опубликована: Июль 9, 2024

Peripheral blood mononuclear cells (PBMC), sourced autologously, offer numerous advantages when procured: easier acquisition process, no in vitro amplification needed, decreased intervention and overall increased acceptability make PBMC an attractive candidate for cell therapy treatment. However, the exact mechanism by which treat diseases remains poorly understood. Immune imbalance is pathological basis of many diseases, with macrophages playing a crucial role this process. research on mechanisms regulating scarce. This study employed co-culture model RAW264.7 to explore macrophages. The results showed that co-culturing led expression inflammatory cytokines anti-inflammatory or culture supernatant. Additionally, pro-inflammatory, tissue matrix-degrading M1 decreased, while anti-inflammatory, matrix-synthesizing, regenerative M2 both monocytes within PBMC. Moreover, co-cultured exhibited significantly p-STAT1/STAT1 ratio, p-STAT6/STAT6 ratio increased. suggests may inhibit macrophage polarization blocking STAT1 signaling cascades promote through activation STAT6 cascades. Overall, sheds light it was found differentiated into presence finding provides experimental evidence use treating especially macrophage-depleting such as osteoarthritis.

Язык: Английский

---

Advances in common in vitro cellular models of pulmonary fibrosis

Immunology and Cell Biology, Год журнала: 2024, Номер 102(7), С. 557 - 569

Опубликована: Май 7, 2024

Abstract The development of in vitro models is essential for a comprehensive understanding and investigation pulmonary fibrosis (PF) at both cellular molecular levels. This study presents literature review an analysis various used scientific studies, specifically focusing on their applications elucidating the pathogenesis PF. Our highlights importance taking approach to studing PF, emphasizing necessity considering multiple cell types organs integrating diverse analytical perspectives. Notably, primary cells demonstrate remarkable growth characteristics gene expression profiles; however, limited availability, maintenance challenges, inability continuous propagation susceptibility phenotypic changes over time significantly limit utility investigation. By contrast, immortalized lines are easily accessible, cultured continuously propagated, although they may have some differences from cells. Furthermore, coculture offer more practical precise method explore complex interactions among cells, tissues organs. Consequently, when developing researchers should thoroughly assess advantages, limitations relevant mechanisms different ensure selection consistent with research objectives.

Язык: Английский

---

Repurposing existing drugs for the treatment ofCOVID-19/SARS-CoV-2: A review of pharmacological effects and mechanism of action

Heliyon, Год журнала: 2024, Номер 10(16), С. e35988 - e35988

Опубликована: Авг. 1, 2024

Язык: Английский

---