Rigor and reproducibility in human brain organoid research: Where we are and where we need to go

Stem Cell Reports, Год журнала: 2024, Номер 19(6), С. 796 - 816

Опубликована: Май 16, 2024

Human brain organoid models have emerged as a promising tool for studying human development and function. These preserve genetics recapitulate some aspects of development, while facilitating manipulation in an vitro setting. Despite their potential to transform biology medicine, concerns persist about fidelity. To fully harness potential, it is imperative establish reliable analytic methods, ensuring rigor reproducibility. Here, we review current analytical platforms used characterize forebrain cortical organoids, highlight challenges, propose recommendations future studies achieve greater precision uniformity across laboratories.

Язык: Английский

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Tissue clearing and its applications in human tissues: A review

View, Год журнала: 2024, Номер 5(2)

Опубликована: Янв. 15, 2024

Abstract In three‐dimensional (3D) space, an unbiased and systemic view of human specimens between structures functions is required. However, conventional histological sections from have made only limited progress in exploring intact information about 3D biological tissues. With significant advances optical physics chemical engineering, state‐of‐the‐art tissue‐clearing methods can revolutionize the subcellular level tissue analysis, thick tissues to organs. The present review summarizes principle clearing so that a trainee researcher implement effective protocols. Furthermore, this highlights existing specific applications, describes imaging strategies, presents various efficient computational approaches for processing visualizing large image data. Finally, potential future directions developing are also discussed.

Язык: Английский

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Tissue clearing and 3D imaging in developmental biology

Development, Год журнала: 2021, Номер 148(18)

Опубликована: Сен. 15, 2021

ABSTRACT Tissue clearing increases the transparency of late developmental stages and enables deep imaging in fixed organisms. Successful implementation these methodologies requires a good grasp sample processing, possibilities offered by image analysis. In this Primer, we highlight how tissue can revolutionize histological analysis processes advise on to implement effective protocols, strategies methods for biology.

Язык: Английский

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Exploring copper (II) porphyrin complexes and their derivatives for electrochemical analysis and biological assessment in the study of breast cancer (MCF-7) cell lines

Environmental Research, Год журнала: 2024, Номер 250, С. 118489 - 118489

Опубликована: Фев. 17, 2024

Язык: Английский

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Ventricular Arrhythmias in Ischemic Cardiomyopathy—New Avenues for Mechanism-Guided Treatment

Cells, Год журнала: 2021, Номер 10(10), С. 2629 - 2629

Опубликована: Окт. 1, 2021

Ischemic heart disease is the most common cause of lethal ventricular arrhythmias and sudden cardiac death (SCD). In patients who are at high risk after myocardial infarction, implantable cardioverter defibrillators effective treatment to reduce incidence SCD ablation therapy can be for with identifiable culprit lesions. Yet, these approaches not always successful come a considerable cost, while pharmacological management often poor ineffective, occasionally proarrhythmic. Advances in mechanistic insights technological innovation have led improved interventional that being evaluated clinically, yet advancement has remained behind. We review basis current provide perspective gaining new centre on complex tissue architecture arrhythmogenic infarct border zone surviving myocytes as source triggers central players re-entry circuits. Identification arrhythmia critical sites characterisation molecular signature unique open avenues targeted off-target effects hampered systemic pharmacotherapy. Such advances line precision medicine patient-tailored therapy.

Язык: Английский

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Optical tissue clearing associated with 3D imaging: application in preclinical and clinical studies

Histochemistry and Cell Biology, Год журнала: 2022, Номер 157(5), С. 497 - 511

Опубликована: Март 2, 2022

Abstract Understanding the inner morphology of intact tissues is one most competitive challenges in modern biology. Since beginning twentieth century, optical tissue clearing (OTC) has provided solutions for volumetric imaging, allowing microscopic visualization thick sections tissue, organoids, up to whole organs and organisms (for example, mouse or rat). Recently, also been introduced clinical settings achieve a more accurate diagnosis with support 3D imaging. This review aims give an overview recent developments OTC imaging illustrate their role field medical diagnosis, specific focus on applications. Graphical abstract

Язык: Английский

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Guidelines on antibody use in physiology research

AJP Renal Physiology, Год журнала: 2024, Номер 326(3), С. F511 - F533

Опубликована: Янв. 18, 2024

Antibodies are one of the most used reagents in scientific laboratories and critical components for a multitude experiments physiology research. Over past decade, concerns about many biological methods, including those that use antibodies, have arisen as several were unable to reproduce data obtained other laboratories. The lack reproducibility could be largely attributed inadequate reporting detailed no or limited verification by authors, production unvalidated antibodies. goal this guideline article is review best practices concerning commonly techniques involving immunoblotting, immunohistochemistry, flow cytometry. Awareness integration will increase rigor these elevate quality

Язык: Английский

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Revisiting Cardiac Biology in the Era of Single Cell and Spatial Omics

Circulation Research, Год журнала: 2024, Номер 134(12), С. 1681 - 1702

Опубликована: Июнь 6, 2024

Throughout our lifetime, each beat of the heart requires coordinated action multiple cardiac cell types. Understanding biology, its intricate microenvironments, and mechanisms that govern their function in health disease are crucial to designing novel therapeutical behavioral interventions. Recent advances single-cell spatial omics technologies have significantly propelled this understanding, offering insights into cellular diversity complex interactions tissue. This review provides a comprehensive overview landscape heart, bridging gap between suspension-based emerging situ approaches, focusing on experimental computational challenges, comparative analyses mouse human systems, rising contextualization cells within niches. As we explore at unprecedented resolution, integrating from both studies will pave way for diagnostic tools therapeutic interventions, ultimately improving outcomes patients with cardiovascular diseases.

Язык: Английский

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Multiscale Three‐Dimensional Evaluation and Analysis of Murine Lung Vasculature From Macro‐ to Micro‐Structural Level

Pulmonary Circulation, Год журнала: 2025, Номер 15(1)

Опубликована: Янв. 1, 2025

ABSTRACT The pulmonary vasculature plays a pivotal role in the development and progress of chronic lung diseases. Due to limitations conventional two‐dimensional histological methods, complexity detailed anatomy blood circulation might be overlooked. In this study, we demonstrate practical use optical serial block face imaging (SBFI), ex vivo microcomputed tomography (micro‐CT), nondestructive for visualization quantification circulation's 3D architecture from macro‐ micro‐structural levels murine samples. We that SBFI can provide rapid, cost‐effective, label‐free mouse macrostructures large vessels. Micro‐CT offers high‐resolution captures microvascular (pre)capillary structures, with microstructural quantification. Optical microscopy techniques such as projection (OPT) light sheet fluorescence microscopy, allows noninvasive, mesoscopic optically cleared lungs, still enabling microscopic reconstruction down precapillary level. By integrating SBFI, micro‐CT, framework understanding circulation, emphasis on vascular pruning rarefaction. Our study showcases applicability complementarity these organ‐level imaging, offering researchers flexibility selecting optimal approach based their specific requirements. conclusion, propose 3D‐directed approaches whole‐organ view health disease, advance our current knowledge diseases affecting vasculature.

Язык: Английский

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The Interaction Between Vasculogenic Mimicry and the Immune System: Mechanistic Insights and Dual Exploration in Cancer Therapy

Cell Proliferation, Год журнала: 2025, Номер unknown

Опубликована: Янв. 26, 2025

ABSTRACT Vasculogenic mimicry (VM) represents a novel form of angiogenesis discovered in numerous malignant tumours recent years. Unlike traditional angiogenesis, VM facilitates tumour blood supply independently endothelial cells by enabling to functional vascular networks. This phenomenon, where replace tubular structures, plays pivotal role growth and metastasis. Tumour progression is influenced variety factors, including immune components. The system serves as critical defence mechanism identifying eliminating abnormal entities, such cells. inevitably reminds us the intricate connection between VM. Indeed, years, some studies have shown that responses related play different regulatory roles formation Therefore, this review provides comprehensive discussion on mechanisms underlying formation, its interplay with system, potential leveraging immunotherapy target

Язык: Английский

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