Опубликована: Янв. 1, 2024
We devise and experimentally demonstrate a method for measuring third order temporal coherence at zero delay of light within fluorescent sample by simultaneous second autocorrelation, without assumptions about the pulse shape.
Язык: Английский
Communications Biology, Год журнала: 2025, Номер 8(1)
Опубликована: Май 23, 2025
Abstract The medial prefrontal cortex (mPFC) is important for higher cognitive functions, including working memory, decision making, and emotional control. In vivo recordings of neuronal activity in the mPFC have been achieved via invasive electrical optical approaches. Here we apply low three-photon imaging mouse at unprecedented depth. Specifically, measure astrocytic Ca 2+ -transient parameters awake head-fixed mice up to a depth 1700 µm. Furthermore, longitudinally record dendritic spine density (0.41 ± 0.07 µm −1 ) deeper than 1 mm week. Using 1650 nm wavelength excite red fluorescent microglia, quantify their processes’ motility (58.9 2% turnover rate) previously unreachable depths (1100 µm). We establish enabling glial with subcellular resolution that will pave way novel discoveries this brain region.
Язык: Английский
Процитировано
0
The Journal of Comparative Neurology, Год журнала: 2024, Номер 532(4)
Опубликована: Апрель 1, 2024
Abstract Comprehensive understanding of interconnected networks within the brain requires access to high resolution information large field views and over time. Currently, methods that enable mapping structural changes entire in vivo are extremely limited. Third harmonic generation (THG) can resolve myelinated structures, blood vessels, cell bodies throughout without need for any exogenous labeling. Together with deep penetration long wavelengths, this enables brain‐mapping fractions small animals Here, we demonstrate THG microscopy allows non‐invasive label‐free an adult vertebrate, Danionella dracula , which is a miniature species cyprinid fish. We show capability multiple regions particular identification major commissural fiber bundles midbrain hindbrain. These features provide readily discernable landmarks navigation regional‐specific neuronal groups even single neurons during experiments. further how technique easily be coupled fluorescence used as comparative tool studies other similar body such zebrafish ( Danio rerio) tetras Trochilocharax ornatus ). This new evidence, building on previous studies, demonstrates size relative transparency, combined unique capabilities microscopy, vertebrate brain.
Язык: Английский
Процитировано
3
Frontiers in Molecular Neuroscience, Год журнала: 2022, Номер 15
Опубликована: Апрель 14, 2022
Rodents have been the dominant animal models in neurobiology and neurological disease research over past 60 years. The prevalent use of rats mice neuroscience has driven by several key attributes including their organ physiology being more similar to humans, availability a broad variety behavioral tests genetic tools, widely accessible reagents. However, despite many advances understanding that achieved using rodent models, there remain limitations questions can be addressed these other mammalian models. In particular, vivo imaging mammals at cell-resolution level remains technically difficult demands large investments time cost. simpler nervous systems non-mammalian allow for precise mapping circuits even whole brain with impressive subcellular resolution. types available spans vertebrates non-vertebrates, so an appropriate model most cell biological neurodegenerative likely exists. A push diversify used could help address current gaps knowledge, complement existing rodent-based bodies work, bring new insight into our human disease. Moreover, are inherent aspects such as lifespan tissue transparency make them specifically advantageous studies. Crispr/Cas9 gene editing decreased cost genome sequencing combined optical microscopy enhances utility specific questions. This review seeks synthesize knowledge established emerging organisms cellular-resolution techniques suggest approaches understand neurodegeneration neurobiological processes. We will summarize tools single scale lead increased consideration research.
Язык: Английский
Процитировано
12
Biomedical Optics Express, Год журнала: 2023, Номер 14(8), С. 4369 - 4369
Опубликована: Июль 20, 2023
Measurements of three-photon action cross-sections for fluorescein (dissolved in water, pH ∼11.5) are presented the excitation wavelength range from 1154 to 1500 nm ∼50 steps. The source is a femtosecond tunable non-collinear optical parametric amplifier, which has been spectrally filtered with 50 full width at half maximum band pass filters. Cube-law power dependance confirmed measurement wavelengths. spectrum found differ both one- and two-photon spectra. cross-section more than an order magnitude larger those 1450 (approximately three times one-photon peak), possibly indicates presence resonance enhancement.
Язык: Английский
Процитировано
7
Elsevier eBooks, Год журнала: 2024, Номер unknown, С. 159 - 193
Опубликована: Янв. 1, 2024
Язык: Английский
Процитировано
2
ACS Applied Engineering Materials, Год журнала: 2024, Номер 2(11), С. 2504 - 2520
Опубликована: Ноя. 9, 2024
Two-photon-excited fluorescence imaging (TPEFI) is a rapidly advancing technique for detecting analytes and visualizing biological conditions in real time. Compared to conventional one-photon excitation, TPEFI offers advantages, such as deeper tissue penetration, reduced photodamage, high sensitivity, superior temporal resolution, making it highly suitable vivo applications. The integration of biostimuli-responsive elements into two-photon fluorophores has enabled the development bioactivatable two-photon-excited small molecules that are effective near-infrared bioimaging monitoring diverse processes diseases. This review highlights recent progress (2019–2024) design application fluorescent probes specifically developed activity-based bioimaging. We provide succinct overview both chemically enzymatically activated by discussing their structural principles, bioresponsive characteristics, absorption emission properties, use vitro specific biomedical
Язык: Английский
Процитировано
2
Biophysical Reviews, Год журнала: 2023, Номер 15(4), С. 709 - 719
Опубликована: Июнь 26, 2023
Язык: Английский
Процитировано
4
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Авг. 28, 2024
Abstract The medial prefrontal cortex (mPFC) is important for higher cognitive functions, including working memory, decision making, and emotional control. In vivo recordings of neuronal activity in the mPFC have been achieved via invasive electrical optical approaches. Here we apply low three-photon imaging mouse at unprecedented depth. Specifically, measure astrocytic Ca 2+ -transient parameters awake head-fixed mice up to a depth 1700 µm. Furthermore, longitudinally record dendritic spine density (0.41 ±0.07 µm -1 ) deeper than 1 mm week. Using 1650 nm wavelength excite red fluorescent microglia, quantify their processes’ motility (58.9 ±2% turnover rate) previously unreachable depths (1100 µm). We establish enabling glial with subcellular resolution that will pave way novel discoveries this brain region.
Язык: Английский
Процитировано
1
Опубликована: Янв. 1, 2024
We study the effect of randomly distributed absorbing tissue on surface sample in multiphoton microscopy. Through numerical simulation, signal-to-background ratio and point spread function system are investigated.
Язык: Английский
Процитировано
0
Nature Methods, Год журнала: 2024, Номер 21(10), С. 1767 - 1769
Опубликована: Окт. 1, 2024
Язык: Английский
Процитировано
0