Hypermut 3: Identifying specific mutational patterns in a defined nucleotide context that allows multistate characters DOI Creative Commons
Zena Lapp, Hyejin Yoon, Brian Foley

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Окт. 29, 2024

Abstract Summary The detection of APOBEC3F- and APOBEC3G-induced mutations in virus sequences is useful for identifying hypermutated sequences. These are not representative viral evolution can therefore alter the results downstream sequence analyses if included. We previously published software Hypermut, which detects hypermutation events relative to a reference. Two versions this method available as webtool. Neither these methods consider multistate characters or gaps alignment. Here, we present an updated, user-friendly web command-line version Hypermut with functionality handle This tool allows straightforward integration into analysis pipelines. As previous tool, while main purpose identify G A events, any mutational pattern context be specified. Availability implementation 3 written Python 3. It at https://github.com/MolEvolEpid/hypermut3 webtool https://www.hiv.lanl.gov/content/sequence/HYPERMUT/hypermutv3.html . Contact [email protected] [email protected]

Язык: Английский

Environmental, socioeconomic, and sociocultural drivers of monkeypox transmission in the Democratic Republic of the Congo: a One Health perspective DOI Creative Commons
Guangyu Lu,

Zeyin Chong,

Enyu Xu

и другие.

Infectious Diseases of Poverty, Год журнала: 2025, Номер 14(1)

Опубликована: Фев. 7, 2025

Язык: Английский

Процитировано

2
Mpox Virus and its ocular surface manifestations DOI
Nikhil Chakravarty,

Darshi Hemani,

Ramya Paravastu

и другие.

The Ocular Surface, Год журнала: 2024, Номер 34, С. 108 - 121

Опубликована: Июль 5, 2024

Язык: Английский

Процитировано

10
Concurrent outbreaks of mpox in Africa—an update DOI

Camila González Beiras,

Emile Malembi,

Roser Escrig-Sarreta

и другие.

The Lancet, Год журнала: 2024, Номер unknown

Опубликована: Дек. 1, 2024

Язык: Английский

Процитировано

9
Establishment of an animal model for monkeypox virus infection in dormice DOI Creative Commons
Gaojie Song, Lingling Cheng, Jun Liu

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Фев. 3, 2025

This study aims to establish an animal model of monkeypox virus (MPXV) infection in dormice through intranasal inoculation. Male aged 4-5 months were selected as experimental subjects and administered different titers MPXV (103.5 PFU, 104.5 105.5 respectively) via nasal instillation. Within 14 days post-infection, clinical indicators such survival rate, body weight changes, respiratory status, mental state continuously monitored. Additionally, tissue samples from the lungs, liver, spleen, trachea each group collected on 5th 10th for titer detection, histopathological analysis was performed lung days. Dormice infected with exhibited typical symptoms appetite loss, continuous reduction, aggravated difficulties, accompanied by lethargy, chills, other manifestations similar human infection. Virological tests further confirmed distribution multiple vital organs dormice, including trachea, particularly significant pathological damage observed tissue. An successfully established inoculation a PFU MPXV, which can be used studying mechanism pharmacology MPXV.

Язык: Английский

Процитировано

1
Novel drug targets for monkeypox: From viral to host proteins DOI Creative Commons
Zhaozhong Zhu, Qin Sun,

Yilun Xu

и другие.

Infectious Medicine, Год журнала: 2025, Номер 4(1), С. 100165 - 100165

Опубликована: Янв. 13, 2025

The ongoing threat of the monkeypox virus (MPXV) underscores need for new antiviral treatments, yet drug targets and candidate therapies are limited. Calculating centrality, conservation, immunogenicity MPXV proteins in network to identify viral targets. Constructing MIP-human protein interaction identifying key human as potential through topology analysis. We constructed a comprehensive protein-protein (PPI) between humans, using data from P-HIPSTer database. This included 113 2 607 MPXV-interacting (MIPs). identified three (OPG054, OPG084, OPG190) drugs, well 95 critical MIPs (most interacting MIPs, MMIPs) within MPXV-human PPI network. Further analysis revealed 31 MMIPs broad-spectrum agents, supported by their involvement other interactions. Functional enrichment indicated roles infection immune-related pathways. In total, we 112 drugs targeting 371 MMIPs, with fostamatinib, trilostane, raloxifene being able inhibit both host proteins. work provides insights into interactions supports development targeted therapies.

Язык: Английский

Процитировано

0
Mpox: Global epidemic situation and countermeasures DOI Creative Commons

Wen-Shuang Hou,

Nan Wu,

Yanzhi Liu

и другие.

Virulence, Год журнала: 2025, Номер 16(1)

Опубликована: Фев. 8, 2025

Mpox, is a zoonotic disease caused by the monkeypox virus and primarily endemic to Africa. As countries gradually stop smallpox vaccination, resistance declining, increasing risk of infection with mpox other viruses. On 14 August 2024, World Health Organization announced that spread constituted public health emergency international concern. Mpox's transmission routes symptoms are complex pose new challenges global health. Several vaccines (such as ACAM2000, JYNNEOS, LC16m8, genetically engineered vaccines) antiviral drugs tecovirimat, brincidofovir, cidofovir, varicella immunoglobulin intravenous injection) have been developed marketed prevent control this disease. This review aims introduce epidemic situation, epidemiological characteristics, physiological pathological preventive measures for in detail, provide scientific basis prevention viruses worldwide.

Язык: Английский

Процитировано

0
Evidence of Mpox Clade IIb Infection in Primary Human Alveolar Epithelium DOI Creative Commons

Thanaphon Namporn,

Suwimon Manopwisedjaroen,

Montien Ngodngamthaweesuk

и другие.

Emerging Microbes & Infections, Год журнала: 2025, Номер unknown

Опубликована: Март 10, 2025

Monkeypox virus (Mpox) has been recognized for causing distinct skin lesions and is primarily transmitted through sexual contact. To date, the transmissibility pathogenesis of Mpox in distal human lung never completely explored. Here transmission pathways tropism on patient-derived air-liquid epithelium (ALE) model fabricated using isolated primary alveolar epithelial cells (hAECs) were investigated. hAECs cultured exposed to clade IIb from patient. DNA, proteins, elucidated polymerase chain reaction (PCR), Western blot high-content fluorescent imaging. Transmission electron microscopy (TEM) was employed systematically observe cellular distribution viral particles. Viral titers determined by TCID50 assay. Innate immune response inflammatory mediators measured Milliplex® multiplex ELISA analysis. Pathology at barrier integrity transepithelial electrical resistance (TEER) The study included mock-infected as control. significantly infected 42.82% total hAEC populations. prominent observed pathology a significant reduction TEER values, loss tight junction protein, presence tunneling nanotubes (TNTs) syncytium morphology. Four stages biogenesis clearly without activation IL-6, MIP1alpha, TNF-α, Galectin-9, although IL-1β subtly promoted. developed ALE versatile infection reflecting respiratory competence Mpox. Postinfection demonstrated damage inflammation, raising concerns about possible evasion virus.

Язык: Английский

Процитировано

0
Overview of monkeypox virus DOI Creative Commons
Kashish Wilson,

G Garima,

Rajat Goyal

и другие.

SCRIPTA MEDICA, Год журнала: 2025, Номер 56(1), С. 185 - 197

Опубликована: Янв. 1, 2025

Monkeypox virus (MPXV) is a close relative of the Variola (smallpox) virus, which was formerly prevalent throughout West and Central Africa. Nonetheless, instances have lately been verified outside Africa in several nonendemic nations. Considering COVID-19 pandemic, World Health Organization (WHO) defined recent monkeypox spread health emergency for public global significance on 23 July 2022. This worldwide community may be danger because increased number confirmed cases. The (MPXV), etiologic agent, isolated from diseased monkeys 1959, its pathogenicity humans first documented 1970s, primarily Western African endemic countries. But 2022, this disease shows extreme return at never-before-seen rate, raising concerns about communicative expansion non-endemic areas potential human-to-human transmission. Healthcare professionals, regulators general people around world must possess substantial expertise such relatively unknown viral diseases to mitigate situation. Here, thorough current pathogenesis overview, epidemiology clinical characteristics, along with therapy presented. review also covers future research fields, vaccine advancements preventive control methods reemerging that currently recognised as crisis health.

Язык: Английский

Процитировано

0
Bioinformatic identification of monkeypox virus phylogenetic gene trees that are representative of its whole-genome phylogenetic tree DOI

El-Miracle Idorenyin Akpan,

Diana Diaz, Malachy Ifeanyi Okeke

и другие.

Virus Genes, Год журнала: 2024, Номер 60(6), С. 635 - 641

Опубликована: Окт. 6, 2024

Язык: Английский

Процитировано

1
Exploring the genomic basis of Mpox virus-host transmission and pathogenesis DOI Creative Commons

Brayden Young,

Stephanie N. Seifert,

Crystal Lawson

и другие.

mSphere, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 14, 2024

Mpox disease, caused by the monkeypox virus (MPXV), was recently classified as a public health emergency of international concern due to its high lethality and pandemic potential. MPXV is zoonotic disease that emerged primarily spread small rodents. Historically, it considered mainly not likely sustain human-to-human transmission. However, worldwide outbreak Clade IIb from 2020 2022 ongoing I epidemics in Democratic Republic Congo surrounding areas are warning human-adapted MPXVs will continually arise. Understanding viral genetic determinants host range, pathogenesis, immune evasion imperative for developing control strategies predicting future Mpox. Here, we delve into genome detail genes involved this rodent-borne human-circulating virus. We compare gene content related Orthopoxviruses, which have narrow ranges, identify potential species-specific pathogenesis tropism. In addition, cover key virulence factor differences distinguish clade lineages. Finally, dissect how genomic reduction through various molecular mechanisms, contributing generation novel lineages with increased human adaptation. This review aims highlight defines species, clades, culminated being elevated national concern.

Язык: Английский

Процитировано

1