In Vitro Modelling of a Typical Dietary Intake in Restrictive Anorexia Nervosa Results in Changes to Gut Microbial Community and Metabolites DOI Creative Commons

Litai Liu,

Carlos Poveda, Paul E. Jenkins

и другие.

Applied Microbiology, Год журнала: 2024, Номер 4(4), С. 1642 - 1660

Опубликована: Дек. 10, 2024

Anorexia nervosa (AN) is a psychiatric illness with harmful physical consequences. Studies have observed differences in the faecal microbiota of patients AN compared to healthy controls. Diet has an impact on gut microbiota, facilitating altered community, such changes could gut–brain axis. In this study, three-stage model system that mimics luminal microbiology large intestine was conducted identify relationships between diet and microbiota. A microbial medium developed provide nutrients more appropriate restricting subtype (R-AN). The inoculated faeces samples were taken compare end products following fermentation control (HC) R-AN medium. Then, 16S amplicon sequencing along flow cytometry–fluorescence situ hybridisation used ascertain Gas chromatography (GC) assess metabolites. There reduced levels SCFA media led fewer total bacteria numbers, less bifidobacteria Rumincoccus proximally, but Clostridium Enterobacteriaceae. Nutrient-deficient resulted neurotransmitter-producing bacteria, butyrate-producing increased protein-utilising all which be maintaining factors AN. provides novel tool for exploring how extreme dietary therefore useful assessing targeted treatments.

Язык: Английский

Gut microbiota-related neuroinflammation at the crossroad of food reward alterations: implications for eating disorders DOI Creative Commons
Sabrina J. P. Huwart, Nuria Morales‐Puerto, Amandine Everard

и другие.

Gut, Год журнала: 2025, Номер unknown, С. gutjnl - 333397

Опубликована: Фев. 17, 2025

The link between gut microbiome and eating behaviours, especially palatable food intake, is a growing focus of scientific investigation. complex ecosystem microorganisms in the influences host metabolism, immune function neurobehavioural signalling. This review explores role neuroinflammation dysregulations food-induced reward signalling potential causal microbiota on these proinflammatory processes. Particular attention given to disorders (ED, specifically anorexia nervosa, binge disorder bulimia nervosa) links with microbiota, alterations neuroinflammation. Finally, we propose modulation as promising therapeutic strategy ED.

Язык: Английский

Процитировано

3
TLR4 identified as a major depressive disorder gene signature mediating effects through multiple immune cells DOI Creative Commons
Jia Wei, Lei Tang, Sijian Wang

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Апрель 11, 2025

Язык: Английский

Процитировано

1
Identification of Six Cerebrospinal Fluid Metabolites Causally Associated with Anorexia Nervosa Risk: A Mendelian Randomization Analysis DOI Open Access
Chao Dai, Xiu‐Wu Bian, Xiaohong Yao

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(7), С. 3248 - 3248

Опубликована: Март 31, 2025

Anorexia nervosa (AN) is a severe psychiatric disorder characterized by substantial heritability and high mortality rate among disorders. While cerebrospinal fluid (CSF) metabolomics has emerged as novel approach to investigating central nervous system pathologies, its specific causal relationship with anorexia remains be fully elucidated. Using genome-wide association study (GWAS) summary statistics for human CSF metabolites AN information from publicly available datasets, we performed two-sample Mendelian randomization (MR) analysis using the inverse-variance weighted (IVW) method primary approach, complemented sensitivity analyses. Through comprehensive of 338 metabolites, identified six significant relationships risk. 1-stearoyl-2-linoleoyl-gpc (18:0/18:2) (OR = 1.09, 95% CI 1.00-1.18) alpha-tocopherol 1.36, 1.00-1.83) showed positive associations, increasing Conversely, sphingomyelin (d18:1/20:0, d16:1/22:0) 0.86, 0.77-0.95), 2,3-dihydroxy-2-methylbutyrate 0.92, 0.86-0.98), N-acetylhistidine oxalate (ethanedioate) 0.83, 0.73-0.94) had protective effects, reducing Sensitivity analyses no evidence horizontal pleiotropy or heterogeneity in MR results. An directionality test Steiger filtering confirmed absence reverse causality, thereby substantiating robustness our findings. These findings suggest that these could serve potential biomarkers early detection highlight therapeutic targets, potentially improving diagnosis intervention strategies this challenging disorder.

Язык: Английский

Процитировано

0
Phosphatidylcholine and frailty: a Mendelian randomization study and immune mediation DOI Creative Commons

Qunhua Han,

Suisui Luo,

Shunmei Huang

и другие.

Archives of Gerontology and Geriatrics, Год журнала: 2025, Номер 135, С. 105863 - 105863

Опубликована: Апрель 17, 2025

Язык: Английский

Процитировано

0
Strengthening causal inference between gut microbiota and prostate cancer: Methodological considerations and recommendations DOI
Junxian Zhao, Junhao Chen,

Zegang Liu

и другие.

Urologic Oncology Seminars and Original Investigations, Год журнала: 2025, Номер 43(7), С. 438 - 439

Опубликована: Апрель 28, 2025

Язык: Английский

Процитировано

0
Causal Relationship Between Gut Microbiota, Blood Metabolites, and Intervertebral Disc Degeneration: A Two‐Step, Two‐Sample Bidirectional Mendelian Randomization Study DOI Creative Commons

Yifan Zheng,

Donglin Yang, Lu‐Yang Wang

и другие.

JOR Spine, Год журнала: 2025, Номер 8(2)

Опубликована: Май 29, 2025

ABSTRACT Background Some studies have shown that gut microbiota may be associated with intervertebral disc degeneration. However, the causal effects between and IVDD whether blood metabolites act as a mediator remain unclear. The objective of this study was to investigate relationship herniation, focus on potential mediating role metabolites. Methods Gut microbiota, metabolites, data were identified from large‐scale genome‐wide association (GWAS) summary data. Then we used Mendelian randomization analysis relationships degeneration, using inverse variance‐weighted method primary outcome measure. Subsequently, conducted sensitivity analyses ascertain robustness results by testing for heterogeneity horizontal pleiotropy. In addition, explored factor in pathway IVDD. Results We 6 taxa strongly incidence herniation. There 8 positive 13 negative genetic liability mediation revealed connections among genus Comamonas B, family Halomonadaceae, UBA6960, mediated ADP glycine ratio, 1,3‐dimethylurate levels, 3‐hydroxy‐2‐methylpyridine sulfate Histidine levels. Each these accounted 7.77%, 9.04%, 12.56%, 11.76%, respectively. Conclusions Our provides evidence supporting certain microbial This focuses specific which suggests they represent targets intervention.

Язык: Английский

Процитировано

0
In Vitro Modelling of a Typical Dietary Intake in Restrictive Anorexia Nervosa Results in Changes to Gut Microbial Community and Metabolites DOI Creative Commons

Litai Liu,

Carlos Poveda, Paul E. Jenkins

и другие.

Applied Microbiology, Год журнала: 2024, Номер 4(4), С. 1642 - 1660

Опубликована: Дек. 10, 2024

Anorexia nervosa (AN) is a psychiatric illness with harmful physical consequences. Studies have observed differences in the faecal microbiota of patients AN compared to healthy controls. Diet has an impact on gut microbiota, facilitating altered community, such changes could gut–brain axis. In this study, three-stage model system that mimics luminal microbiology large intestine was conducted identify relationships between diet and microbiota. A microbial medium developed provide nutrients more appropriate restricting subtype (R-AN). The inoculated faeces samples were taken compare end products following fermentation control (HC) R-AN medium. Then, 16S amplicon sequencing along flow cytometry–fluorescence situ hybridisation used ascertain Gas chromatography (GC) assess metabolites. There reduced levels SCFA media led fewer total bacteria numbers, less bifidobacteria Rumincoccus proximally, but Clostridium Enterobacteriaceae. Nutrient-deficient resulted neurotransmitter-producing bacteria, butyrate-producing increased protein-utilising all which be maintaining factors AN. provides novel tool for exploring how extreme dietary therefore useful assessing targeted treatments.

Язык: Английский

Процитировано

0