In Vitro Modelling of a Typical Dietary Intake in Restrictive Anorexia Nervosa Results in Changes to Gut Microbial Community and Metabolites DOI Creative Commons

Litai Liu,

Carlos Poveda, Paul E. Jenkins

et al.

Applied Microbiology, Journal Year: 2024, Volume and Issue: 4(4), P. 1642 - 1660

Published: Dec. 10, 2024

Anorexia nervosa (AN) is a psychiatric illness with harmful physical consequences. Studies have observed differences in the faecal microbiota of patients AN compared to healthy controls. Diet has an impact on gut microbiota, facilitating altered community, such changes could gut–brain axis. In this study, three-stage model system that mimics luminal microbiology large intestine was conducted identify relationships between diet and microbiota. A microbial medium developed provide nutrients more appropriate restricting subtype (R-AN). The inoculated faeces samples were taken compare end products following fermentation control (HC) R-AN medium. Then, 16S amplicon sequencing along flow cytometry–fluorescence situ hybridisation used ascertain Gas chromatography (GC) assess metabolites. There reduced levels SCFA media led fewer total bacteria numbers, less bifidobacteria Rumincoccus proximally, but Clostridium Enterobacteriaceae. Nutrient-deficient resulted neurotransmitter-producing bacteria, butyrate-producing increased protein-utilising all which be maintaining factors AN. provides novel tool for exploring how extreme dietary therefore useful assessing targeted treatments.

Language: Английский

Gut microbiota-related neuroinflammation at the crossroad of food reward alterations: implications for eating disorders DOI Creative Commons
Sabrina J. P. Huwart, Nuria Morales‐Puerto, Amandine Everard

et al.

Gut, Journal Year: 2025, Volume and Issue: unknown, P. gutjnl - 333397

Published: Feb. 17, 2025

The link between gut microbiome and eating behaviours, especially palatable food intake, is a growing focus of scientific investigation. complex ecosystem microorganisms in the influences host metabolism, immune function neurobehavioural signalling. This review explores role neuroinflammation dysregulations food-induced reward signalling potential causal microbiota on these proinflammatory processes. Particular attention given to disorders (ED, specifically anorexia nervosa, binge disorder bulimia nervosa) links with microbiota, alterations neuroinflammation. Finally, we propose modulation as promising therapeutic strategy ED.

Language: Английский

Citations

2
TLR4 identified as a major depressive disorder gene signature mediating effects through multiple immune cells DOI Creative Commons
Jia Wei, Lei Tang, Sijian Wang

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 11, 2025

Language: Английский

Citations

0
Strengthening causal inference between gut microbiota and prostate cancer: Methodological considerations and recommendations DOI
Junxian Zhao, Junhao Chen,

Zegang Liu

et al.

Urologic Oncology Seminars and Original Investigations, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
Identification of Six Cerebrospinal Fluid Metabolites Causally Associated with Anorexia Nervosa Risk: A Mendelian Randomization Analysis DOI Open Access
Chao Dai, Xiu‐Wu Bian, Xiaohong Yao

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 3248 - 3248

Published: March 31, 2025

Anorexia nervosa (AN) is a severe psychiatric disorder characterized by substantial heritability and high mortality rate among disorders. While cerebrospinal fluid (CSF) metabolomics has emerged as novel approach to investigating central nervous system pathologies, its specific causal relationship with anorexia remains be fully elucidated. Using genome-wide association study (GWAS) summary statistics for human CSF metabolites AN information from publicly available datasets, we performed two-sample Mendelian randomization (MR) analysis using the inverse-variance weighted (IVW) method primary approach, complemented sensitivity analyses. Through comprehensive of 338 metabolites, identified six significant relationships risk. 1-stearoyl-2-linoleoyl-gpc (18:0/18:2) (OR = 1.09, 95% CI 1.00-1.18) alpha-tocopherol 1.36, 1.00-1.83) showed positive associations, increasing Conversely, sphingomyelin (d18:1/20:0, d16:1/22:0) 0.86, 0.77-0.95), 2,3-dihydroxy-2-methylbutyrate 0.92, 0.86-0.98), N-acetylhistidine oxalate (ethanedioate) 0.83, 0.73-0.94) had protective effects, reducing Sensitivity analyses no evidence horizontal pleiotropy or heterogeneity in MR results. An directionality test Steiger filtering confirmed absence reverse causality, thereby substantiating robustness our findings. These findings suggest that these could serve potential biomarkers early detection highlight therapeutic targets, potentially improving diagnosis intervention strategies this challenging disorder.

Language: Английский

Citations

0
Phosphatidylcholine and frailty: a Mendelian randomization study and immune mediation DOI Creative Commons

Qunhua Han,

Suisui Luo,

Shunmei Huang

et al.

Archives of Gerontology and Geriatrics, Journal Year: 2025, Volume and Issue: unknown, P. 105863 - 105863

Published: April 1, 2025

Language: Английский

Citations

0
In Vitro Modelling of a Typical Dietary Intake in Restrictive Anorexia Nervosa Results in Changes to Gut Microbial Community and Metabolites DOI Creative Commons

Litai Liu,

Carlos Poveda, Paul E. Jenkins

et al.

Applied Microbiology, Journal Year: 2024, Volume and Issue: 4(4), P. 1642 - 1660

Published: Dec. 10, 2024

Anorexia nervosa (AN) is a psychiatric illness with harmful physical consequences. Studies have observed differences in the faecal microbiota of patients AN compared to healthy controls. Diet has an impact on gut microbiota, facilitating altered community, such changes could gut–brain axis. In this study, three-stage model system that mimics luminal microbiology large intestine was conducted identify relationships between diet and microbiota. A microbial medium developed provide nutrients more appropriate restricting subtype (R-AN). The inoculated faeces samples were taken compare end products following fermentation control (HC) R-AN medium. Then, 16S amplicon sequencing along flow cytometry–fluorescence situ hybridisation used ascertain Gas chromatography (GC) assess metabolites. There reduced levels SCFA media led fewer total bacteria numbers, less bifidobacteria Rumincoccus proximally, but Clostridium Enterobacteriaceae. Nutrient-deficient resulted neurotransmitter-producing bacteria, butyrate-producing increased protein-utilising all which be maintaining factors AN. provides novel tool for exploring how extreme dietary therefore useful assessing targeted treatments.

Language: Английский

Citations

0