CRISPR/Cas9 therapeutics: progress and prospects

Signal Transduction and Targeted Therapy, Год журнала: 2023, Номер 8(1)

Опубликована: Янв. 16, 2023

Abstract Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) gene-editing technology is the ideal tool of future for treating diseases by permanently correcting deleterious base mutations or disrupting disease-causing genes with great precision and efficiency. A variety efficient Cas9 variants derivatives have been developed to cope complex genomic changes that occur during diseases. However, strategies effectively deliver CRISPR system diseased cells in vivo are currently lacking, nonviral vectors target recognition functions may be focus research. Pathological physiological resulting from disease onset expected serve as identifying factors targeted delivery targets gene editing. Diseases both varied complex, choice appropriate methods different important. Meanwhile, there still many potential challenges identified when targeting CRISPR/Cas9 treatment. This paper reviews current developments three aspects, namely, type, vector, characteristics. Additionally, this summarizes successful examples clinical trials finally describes possible problems associated applications.

Язык: Английский

---

A Review of the Most Common and Economically Important Diseases That Undermine the Cultivation of Tomato Crop in the Mediterranean Basin

Agronomy, Год журнала: 2021, Номер 11(11), С. 2188 - 2188

Опубликована: Окт. 29, 2021

Tomato (Solanum lycopersicum L.), family Solanaceae, has become in the past fifty years one of most important and extensively grown horticultural crops Mediterranean region throughout world. In 2019, more than 180 million tonnes tomato have been produced worldwide, out which around 42 countries. Due to its genetic properties, is afflicted by numerous plant diseases induced fungal, bacterial, phytoplasma, virus, viroid pathogens. Not only inheritance great importance management pathogens, but equally as are also present climate changes, recently revised phytopathological control measures, globalization seed industry. Thus, recognition symptoms knowledge distribution spread disease methods for early detection pathogens major prerequisites a successful disease. this review, we will describe main area that impact mostly yield provide current perspective measures necessary their management.

Язык: Английский

---

Off-target effects in CRISPR/Cas9 gene editing

Frontiers in Bioengineering and Biotechnology, Год журнала: 2023, Номер 11

Опубликована: Март 9, 2023

Gene editing stands for the methods to precisely make changes a specific nucleic acid sequence. With recent development of clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system, gene has become efficient, convenient and programmable, leading promising translational studies clinical trials both genetic non-genetic diseases. A major concern in applications CRISPR/Cas9 system is about its off-target effects, namely deposition unexpected, unwanted, or even adverse alterations genome. To date, many have been developed nominate detect sites CRISPR/Cas9, which laid basis successful upgrades derivatives with enhanced precision. In this review, we summarize these technological advancements discuss current challenges management effects future therapy.

Язык: Английский

---

CRISPR/Cas: a Nobel Prize award-winning precise genome editing technology for gene therapy and crop improvement

Journal of Zhejiang University SCIENCE B, Год журнала: 2021, Номер 22(4), С. 253 - 284

Опубликована: Апрель 1, 2021

Язык: Английский

---

Plant drought stress tolerance: understanding its physiological, biochemical and molecular mechanisms

Biotechnology & Biotechnological Equipment, Год журнала: 2021, Номер 35(1), С. 1912 - 1925

Опубликована: Янв. 1, 2021

Plants are vulnerable to many environmental constraints which include drought, salinity, extreme temperatures, heavy metals, etc., thereby posing damage the development and yield of major crops. Of these stresses, drought represents a severe threat plant productivity in agriculture. Drought stress reduces by affecting key metabolic pathways. The capability plants switch on or off series genes result alterations physiological morphological attributes, allowing escape, tolerate avoid stress. Different genes, transcription factors signal transduction pathways induced Advances genome editing technologies have revolutionized agriculture sector using more accurate engineering techniques for targeted crop traits. Clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) technology is robust efficient approach developing plant/crop varieties tolerant different climatic changes. use new like CRISPR facilitates creation species with improved tolerance. Thus this review aims elucidate basic responses mechanisms adaptation attainment

Язык: Английский

---

Advances of medical nanorobots for future cancer treatments

Journal of Hematology & Oncology, Год журнала: 2023, Номер 16(1)

Опубликована: Июль 14, 2023

Early detection and diagnosis of many cancers is very challenging. Late stage a cancer always leads to high mortality rates. It imperative develop novel more sensitive effective therapeutic methods for treatments. The development new treatments has become crucial aspect medical advancements. Nanobots, as one the most promising applications nanomedicines, are at forefront multidisciplinary research. With progress nanotechnology, nanobots enable assembly deployment functional molecular/nanosized machines increasingly being utilized in treatment. In recent years, various practical have transitioned from theory practice, vitro experiments vivo applications. this paper, we review analyze advancements treatments, with particular emphasis on their key fundamental features drug delivery, tumor sensing diagnosis, targeted therapy, minimally invasive surgery, other comprehensive At same time, discuss challenges potential research opportunities revolutionizing future, expected sophisticated capable performing multiple functions tasks, ultimately becoming true nanosubmarines bloodstream.

Язык: Английский

---

CRISPR/Cas-mediated plant genome editing: outstanding challenges a decade after implementation

Trends in Plant Science, Год журнала: 2023, Номер 28(10), С. 1144 - 1165

Опубликована: Июнь 16, 2023

The discovery of the CRISPR/Cas genome-editing system has revolutionized our understanding plant genome. been used for over a decade to modify genomes study specific genes and biosynthetic pathways as well speed up breeding in many species, including both model non-model crops. Although is very efficient genome editing, bottlenecks challenges slow down further improvement applications. In this review we discuss that can occur during tissue culture, transformation, regeneration, mutant detection. We also opportunities provided by new CRISPR platforms applications related gene regulation, abiotic biotic stress response improvement, de novo domestication plants.

Язык: Английский

---

Computational Tools and Resources for CRISPR/Cas Genome Editing

Genomics Proteomics & Bioinformatics, Год журнала: 2022, Номер 21(1), С. 108 - 126

Опубликована: Март 24, 2022

The past decade has witnessed a rapid evolution in identifying more versatile clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) nucleases and their functional variants, as well developing precise CRISPR/Cas-derived genome editors. programmable robust features of the editors provide an effective RNA-guided platform for fundamental life science research subsequent applications diverse scenarios, including biomedical innovation targeted crop improvement. One most essential principles is to guide alterations genomic sequences or genes intended manner without undesired off-target impacts, which strongly depends on efficiency specificity single RNA (sgRNA)-directed recognition DNA sequences. Recent advances empirical scoring algorithms machine learning models have facilitated sgRNA design prediction. In this review, we first briefly introduce different CRISPR/Cas tools that should be taken into consideration achieve specific purposes. Secondly, focus computer-assisted resources are widely used designing sgRNAs analyzing CRISPR/Cas-induced on- mutations. Thirdly, insights limitations available computational would help researchers field further optimization. Lastly, suggest simple but workflow choosing applying web-based editing.

Язык: Английский

---

Comprehensive review of CRISPR-based gene editing: mechanisms, challenges, and applications in cancer therapy

Molecular Cancer, Год журнала: 2024, Номер 23(1)

Опубликована: Янв. 9, 2024

Abstract The CRISPR system is a revolutionary genome editing tool that has the potential to revolutionize field of cancer research and therapy. ability precisely target edit specific genetic mutations drive growth spread tumors opened up new possibilities for development more effective personalized treatments. In this review, we will discuss different CRISPR-based strategies have been proposed therapy, including inactivating genes tumor growth, enhancing immune response cells, repairing cause cancer, delivering cancer-killing molecules directly cells. We also summarize current state preclinical studies clinical trials highlighting most promising results challenges still need be overcome. Safety delivery are important therapy become viable option. limitations overcome, such as off-target effects, safety, site. Finally, provide an overview opportunities in future directions development. change landscape research, review aims overcome realize potential.

Язык: Английский

---

Host-mediated gene engineering and microbiome-based technology optimization for sustainable agriculture and environment

Functional & Integrative Genomics, Год журнала: 2023, Номер 23(1)

Опубликована: Фев. 8, 2023

Язык: Английский

---